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Showing 1 to 18 of 18 for “"picornavirus"”.
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Sensing of Picornavirus Infections
… virus (EMCV) and coxsackievirus B3 (CVB3), picornaviruses of the cardiovirus and the enterovirus genus respectively, are sensed by both MDA-5 and RIG-I. Sensing of these viruses in macrophages leads to the expression of type I IFN early after infection with IFNβ expression reduced in the …
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A Proteomic Approach to Elucidating the Function of Picornavirus 2A Protease
… complex, apoptosis, and innate immune responses. Picornaviruses are the most dominant human disease-causing viruses and present an excellent clinical target for research studies into their molecular mechanisms. Picornaviruses have an RNA genome that is translated as a single polyprotein, which is …
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Polyadenylation of ribosomal RNA in response to picornavirus infection in honey bees (Apis mellifera)
At least eighteen different picornaviruses can infect honey bees (Apis mellifera). The combination of the virulence of these pathogens as well as their transmissibility through Varroa destructor, an ectoparasitic mite, can cause severe declines in honey bee populations. Polyadenylated (polyA) rRNA …
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Site-Directed Mutagenesis of Southern Bean Mosaic Virus (Cowpea Strain): in Search of a Viral Protease
… The elements within polyproteins of the picornavirus superfamily members have a conserved order: Vpg (viral protein, genome-linked)-protease-replicase. Amino acid sequence homologies indicate that the 105 kDa protein of SBMV contains a replicase very similar to those identified in …
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Adenosine triphosphate is an allosteric inhibitor of coxsackievirus B3 3Dpol
Picornaviruses pose a significant threat to human and animal health, but at present there are no drugs to prevent or treat picornaviral infections. However, intensive study of the picornaviral lifecycle has revealed several promising pharmacological targets, including the RNA-dependent RNA …
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Pharmacology of novel approaches designed to target picornaviral infections
… at least as prevalent as EVs. Currently, no antipicornavirus drugs are used clinically and the large number of viruses precludes vaccination. New drugs are therefore required. Picornavirus infection exploits viral and host proteins, lipids and cellular systems, all potentially targetable by …
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Factors That Influence Mammalian Enteric Virus Infection
… virus co-infection and how bacteria influence picornavirus infection. Recent studies determined several modes of RNA virus transmission exist outside of canonical pathways, including en bloc transmission of multiple viruses into a single cell via bacteria or host-derived membrane vesicles. …
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Studio dell’attività antivirale e/o anti-infiammatoria di nuove molecole di sintesi e/o di farmaci noti
… as well as to evaluate the cytotoxic and anti-picornavirus activities of a class of novel synthetic quinoxaline (Part I). Part of the second and all the third year was spent at the NIAID/NIH, Hamiltion MT, USA, in the laboratory of persistent viral diseases under the supervision of dr. Bruce …
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Assay and Purification of Coxsackievirus B3
… the purification for Coxsackie virus B3 (CB3), a Picornavirus provide the highest degree of purity with the greatest yield. The procedure. Quantitative methods for the assay and purification of CB3 were developed. CB3 was propagated in buffalo green monkey (BGM) cells in tissue culture. The virus …
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Role of Hypoxia-inducible Factor in Kidney Cancer
… ccRCC cells. We also demonstrate that the picornavirus EMCV (Encephalomyocarditis virus) efficiently destroys ccRCC tumour cells in an NF-kappaB- and HIFalpha-dependent manner both in vitro and in a mouse xenograft model. This work provides pre-clinical evidence for the potential use of …
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The effect of Coxsackie virus A9 infection on nuclear and nucleolar proteins
… To investigate if this is a general feature of picornaviruses infection, coxsackie virus A9 (CAV-9) was studied. This is a typical member of the large and most medically-important picornavirus genera Enterovirus, which is genetically divergent from Parechovirus. Using an EGFP-PSPC-1 fusion, we …
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Evasion of RIG-I/MDA5 and TLR3-mediated innate immunity by hepatitis A virus
… Hepatitis A virus (HAV), a hepatotropic picornavirus, is capable of blocking IRF3 activation and type I IFN expression in cell culture, but the exact mechanism(s) remains undefined. Our studies revealed that HAV disrupts RIG-I/MDA5-mediated induction of type I IFN through proteolysis of …
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Coronavirus Replicase Proteins: Multifunctional Mediators of Replication and Innate Immunity Evasion
… which is cleaved by the papain-like (PLP) and picornavirus 3C-like (3CLpro) proteases. The nsps modify host membranes to produce double membrane vesicles (DMVs) upon which the replicase-transcriptase assembles and synthesizes viral RNA. nsp3, nsp4, and nsp6 are integral membrane proteins …
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The roles of papain-like protease-related proteins in viral replication and host immunity
… recombination event, in which the chimeric picornavirus-enterovirus G expresses the PLP gene, homologous to torovirus (ToV) PLPs. Like other nidoviral PLPs, the recombinant ToV-PLP was proved to be a highly active deubiquinase/deISGylase and potent innate immune antagonist. After PLP …
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Characterisation of two aphid picorna-like viruses
… is serologically distantly related to two insect picornaviruses, cricket paralysis virus (CrPV) and Drosophila C virus. No nucleic acid homology was detected between ALPV cDNA and CrPV by dot-blot hybridization. ALPV is serologically unrelated to seven other insect picornalike viruses. RhPV is …
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Poliovirus Replication: Interactions with Cytoplasmic Membranes and with Compounds Which Affect Virus Biosynthesis
<p>Picornavirus multiplication is intimately associated with intracellular membranes and affects their production and distribution within the cell. We have shown that the density distribution of cytoplasmic membranes separated by isopycnic centrifugation in discontinuous sucrose gradients is …
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Investigating the functional roles of the viral frameshift protein 2B* in EMCV infection
… in EMCV-induced cell lysis relative to other picornaviruses.
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Senecavirus A: Epidemiology, Pathogenesis and Infection Dynamics
<p>Senecavirus A (SVA) is a positive sense single-stranded virus that belongs to the genus <em>Senecavirus</em> within the family <em>Picornaviridae</em>. Senecavirus has been present in US swine population since 1988. However, there have been only a few reports of SVA associated with disease in …