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Showing 1 to 9 of 9 for “"photolyase"”.
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Ultrafast Dynamics of Flavin Cofactor in Photolyase/Cryptochrome Family
… of flavin cofactor in four redox states in photolyase/cryptochrome family proteins. In photolyase, the antenna molecule MTHF absorbs a photon and transfer the excitation energy to the catalytic cofactor FADH¯ to enhance the DNA-repair efficiency. With the femtosecond-resolved fluorescence …
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Photolyase: Its Damaged DNA Substrate and Amino Acid Radical Formation During Photorepair
… the most abundant primary lesion in DNA. Photolyase, a light-activated enzyme, catalytically repairs these lesions. Although many steps in the photolyase-mediated repair process have been mapped, details of the mechanism remain cryptic. Difference FT-IR spectroscopy was employed to obtain …
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Biochemical and Molecular Characterizations of a Novel DNA Repair Enzyme, CPD -Photolyase, and Late Promoters of Fowlpox Virus
To further exploit FPV as a vector, characterization of strong promoters were attempted using a lacZ gene based reporter assay. The transcriptional element of ORF P190 appeared to be the strongest. In contrast, the promoter associated with the ORF, P180, was the weakest. Interestingly, a novel …
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Discovery of an extracellular stress sensory protein in Beauveria bassiana and identification of photolyase encoding phr-1 sequences in five entomopathogenic fungi
… temperature were studied here. A region of the photolyase gene (phr-1), encoding cyclobutane pyrimidine dimer photolyase (CPD-PHR), an enzyme pivotal to DNA repair, was cloned, sequenced and identified for species of the genera Beauveria, Isaria, Lecanicillium, Metarhizium and Tolypocladium. The …
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Excited state charge redistribution and dynamics of flavins, flavorproteins, and their cofactors
… to the light activated DNA repair enzyme DNA photolyase have been studied previously by this technique. This work focuses on the catalytically active form, the two-electron reduced anion. To facilitate analysis of this experiment, the Stark spectra of a simple flavin derivative that is soluble …
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Cloning of "Animal Cryptochrome" cDNA from the Model Organism <i>CHLAMYDOMONAS REINHARDTII</i> for Functional Analysis of Its Protein Product
… even higher sequence similarity with the 6-4 DNA photolyase of <i>Arabidopsis</i>. DNA photolyases are involved in the repair of UV light-induced DNA damage using the energy of blue light. In order to determine, if the “animal cryptochrome” gene of <i>C. reinhardtii</i> actually encodes a 6-4 DNA …
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Blue light-dependent development of the filamentous fungus Aspergillus nidulans
… bezeichnetes Gen, das für ein Cryptochrom- bzw. Photolyase-artiges Protein kodieren könnte. Eingehende experimentelle Untersuchungen zeigten, dass CryA von A. nidulans ein einzigartiges Protein der Cryptochrom/Photolyase-ähnlichen Proteinfamilie darstellt, da es aufgrund seiner dualen …
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UVB vermittelte DNA-Schäden und Immunsuppression in humanen dendritischen Zellen
… CPD konnte durch die Behandlung mit Photosomen (Photolyase von Anacystis nidulans verpackt in Liposomen) und photoreaktivierendem Licht wieder repariert werden. Die Reparatur der CPD führte jedoch nicht zu einer Wiederherstellung der allostimulatorischen Funktion der hDZ. <br>In einem weiteren …
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INNOVATIVE APPROACHES TO PLANT PROTECTION AND PRODUCE SAFETY THROUGH HIGH-PRECISION PLANT MANAGEMENT SYSTEMS
… upregulation in UV-damage endonuclease and photolyase related genes, compared to the others: 222W Low dose (UVC treatment using a 222 nm lamp with dose 0.056 kJ/m2). Beyond fungal diseases, viral infections remain a major constraint to the productivity of solanaceous crops. In tomato …