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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"photolabile"”.
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Characterization of Binding of Azidopyrene, a Hydrophobic, Photolabile Probe, to Escherichia Coli and Salmonella Typhimurium
The association of azidopyrene, a hydrophobic, photolabile molecule, with whole cells of Escherichia coli and Salmonella typhimurium was studied. An assay is described to quantitate irreversible light-dependent binding to cells. This binding has little or no effect on proline uptake, ATP levels, or …
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Conjugated Estrogen Receptor Probes: An Evaluation of Fluorophores, Photolabile Groups, Estrogenic Ligands, and the Nature of the Attaching Chain
A good correlation exists between the level of estrogen receptor in breast tumors, and their responsiveness to hormone therapy. However, the correlation is not perfect; about 40% of women with measurable tumor receptor levels do not respond to hormone treatment. The work described herein was …
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Synthese und Photochemie von photoaktivierbaren Biomolekülen
… Bei den caged Verbindungen handelt es sich um photolabile inaktive Derivate von biologisch aktiven Molekülen, aus denen durch ultraviolettes Licht mit Hilfe einer photochemischen Reaktion die natürliche, biologisch aktive Substanz schnell freigesetzt werden kann. Im Rahmen der vorliegenden …
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Synthesis and biological evaluation of potential DNA cross-linking agents based on pyrrolizidine alkaloids
… The synthesis of a potential water soluble photolabile protecting group is also described. In addition, the biological evaluation of the synthesized pyrrolizidine progenitors was investigated.
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Synthesis, Characterization and In-vivo Testing of Photoactivatable Insulin Depots for Continuously Variable and Minimally Invasive Insulin Delivery
… was created by linking insulin covalently to photolabile caging moieties. Transcutaneous irradiation breaks the bond to release insulin from the depot into the systemic circulation. Chapter 3 describes the first successful testing on our PAD technology in diabetic animal models. In Chapters 2 …
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Functional polymer layers with protected amines
… and develop a polymer system based on suitable photolabile units and in addition having anchoring groups which attach on specific substrates like gold. In this terpolymer concept was aimed for which consists of three components with particular functions in suitable molar ratios, which allow the …
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Potential prebiotic roles of (amino-)acylation in the synthesis and function of RNA
… of the nucleobase exocyclic amines and a photolabile solid-phase linker group that allowed partial on-column deprotection. The synthesis of the 2'/3'-O-acetyl and 2'/3'-O-TBDMS phosphoramidites, in addition to preparation of a photolabile solid-phase support, are described. With the …
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Nanoparticle-chaperoned urinary 'synthetic biomarkers' for profiling proteases in cancer
… peptide fragments are pre-designed with internal photolabile triggers that un-cage isobaric peptide mass tags optimized for multiplexed LC MS/MS quantification. Results demonstrate that such peptide 'synthetic biomarker' panels uncover unique proteolytic signatures that can be correlated with …
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Caged phosphopeptides and phosphoproteins : agents in unraveling complex biological pathways
… and the roles of phosphoproteins include photolabile caged analogs of bioactive species. Caged compounds are ideal chemical probes for studying cellular signaling because they afford researchers spatial and temporal control over the release of targeted effector molecules. Ligands or …
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Caged phosphopeptides and phosphoproteins : probes to dissect the role of phosphorylation in complex signaling pathways
… control is accomplished by the photolysis of a photolabile precursor, a caged phosphopeptide. The synthesis and application of NI-Fmoc-protected 1-(2-nitrophenyl) ethyl (NPE) caged phosphothreonine, serine, and tyrosine building blocks facilitate the straightforward assembly of any caged …
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Matrix Isolation of Aryl(trifluoromethyl)carbenes, Heteroaryl(trifluoromethyl)carbenes and Phenylbis(trifluoromethyl)carbenes
… - 5 -pyridyl(trifluoromethyl)carbene, was more photolabile and could be converted to otherintermediates with comparatively short term irradiation. All the studied pyridylcarbenes havetriplet ground states. Although they were photostable, phenyl(trifluoromethyl)biscarbenesattracted our attention …
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Development, Validation, and Application of Methods for High Time-Response Measurement of Gaseous Atmospheric Chlorinated Species
… at sunrise and assessed the contribution of photolabile Cl precursors to radical formation. Total gaseous chlorine (TClg) measurements can illuminate unknown sources of Cl to the atmosphere. Techniques for measuring TClg have been limited to offline analysis of extracted filters and do not …
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Effects of glycosylation on the structure and fibrillization of prion protein fragments
… of this peptide was developed using a synthetic photolabile linker and a "fibril inhibitory unit" positioned at the N-terminus of the peptide. This stabilized the fragment as a soluble monomeric species until photoactivation, which triggered the peptide self-assembly.
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Transition metal complexes of pentadentate ligands
… derivatives of Co(X-SALEN) which happen to be photolabile. The geometry around the cobalt atom in R-Co-(SALDIPN) was concluded to be pseudo-octahedral. Reasons for certain anomalies (i.e., magnetic moments, etc.) were discussed in detail.
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The photocatalytic degradation of phenolic compounds
… however, is itself photolabile in daylight giving 3-bromo-2,5-dihydroxybenzonitrile in aqueous solution. The analogous reaction does not occur for 3,5-dibromo-4-hydroxybenzonitrile, the difference in behaviour being attributed to differences in the electronic spectra …
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Development of Stimulus-Responsive Ligands for the Modulation of Copper and Iron Coordination
… (PC-HAPI) is masked with a photolabile trans-cinnamic acid protecting group that releases umbelliferone, a UV-absorbing, antioxidant coumarin along with a chelating agent upon UV irradiation. In addition to the antioxidant effects of the coumarin, the released chelator (HAPI) …
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New chemical and biosynthetic methodologies for the study of lanthipeptides
… Chapter 2 will discuss a method to introduce a photolabile linker between lanthipeptide leader and core regions. Posttranslational modification of the lanthipeptide by its cognate synthetase in vitro, followed by UV-light mediated removal of the leader peptide, yielded the mature lanthipeptide. …
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Engineering a Photo-Control Mechanism for RNA Interference
… which involves the covalent attachment of photolabile compounds to the effector NA species that blocks bioactivity until exposed to near UV-light. This work demonstrates that fully-2’-fluorinated nucleic acids (FNAs) can elicit RNAi and that these effectors are resistant to sugar-specific …
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Novel Photochemical Methodologies for Diversity Oriented Synthesis and Screening of Combinatorial Libraries
… on the photorelease of externally sensitized photolabile tags. The encoding tags can be released into solution only when a binding event occurs between the ligand and the receptor, equipped with an electron transfer sensitizer. The released tags are analyzed in solution revealing the identity …
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The formation and characterisation of micro- and nanostructured surfaces through combinations of top-down and bottom-up fabrication methodologies
… synthetic hairpin oligonucleotides carrying a photolabile group, on a silicon surface. Once immobilised on a surface, the oligonucleotides are patterned using photolithography to leave exposed single strands, which, through the specific assembly properties of DNA, are used to direct the …
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