Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"photocrosslinking"”.
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Study of Glycan-Mediated Interactions by Photocrosslinking GlcNAc and GalNAc Analogs
… results I have on GalNDAz incorporation and photocrosslinking, which indicate that GalNDAz may be incorporated into cellular glycans. Chapter 1 introduces the background of this thesis, including basics of glycans and lectins, metabolic glycan engineering, photocrosslinking groups and …
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Polyelectrolyte multilayers incorporating photocrosslinking polymers for controlling 2- and 3-dimensional structure
Polyelectrolyte multilayer (PEM) assembly is a technology for building thin films with a number of useful and novel functionalities. PEMs interact favorably with proteins and other biomolecules making them potentially valuable as biomaterials. Many systems of polymers have been studied for use in …
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Development of New Photocrosslinking Approaches to Discover Binding Partners of O-GlcNAc-Modified Proteins
… reported a method to incorporate the diazirine photocrosslinking group onto O-GlcNAc residues in cellular proteins. Photocrosslinking O-GlcNAc, O-GlcNDAz, yields covalent crosslinking between O-GlcNAc-ylated proteins and their binding partners, which further analysis can confirm these …
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Application of Genetically-Encoded Photoactivatable Crosslinkers to Map Ligand-Binding Sites on G Protein-Coupled Receptors
… in the human genome. A general cellbased photocrosslinking approach was developed to investigate the binding interfaces necessary for the formation of GPCR signaling complexes. Amber codon suppression was extended to facilitate the incorporation of photoactivatable unnatural amino acids, …
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Design and synthesis of probes for detection of protein-protein interaction and RNA localization
… probes were synthesized and tested for photocrosslinking activity to investigate the efficiency of photocrosslinking in these systems. Three series of small molecule probes were synthesized for the selection of ribozymes from a random sequence pool. Solid-phase immobilized fluorescein …
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Defining cellular microenvironments using multiphoton lithography
… using multiphoton excitation is applied to the photocrosslinking of proteins, providing the means to organize bioactive species into well-defined 3D microenvironments. Further, conditions have been identified that enable microfabrication to be performed in the presence of cells -- allowing cell …
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Synthesis And Application Of Atp Analogs For Phosphorylation-Dependent Kinase-Substrate Crosslinking
… To this end we developed -phosphate modified ATP photocrosslinking analogs ATP-ArN3 and ATP-BP, that crosslink substrate and kinase in a phosphorylation dependent manner. We have successfully demonstrated that ATP-ArN3 and ATP-BP can be used with natural kinase and substrates using cell lysates in …
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A potential route to hydrogel multifunctionalization utilizing encapsulation of acrylate-conjugated streptavidin
… acrylate hydrogels. Such gels were made by photocrosslinking solutions of APN and streptavidin conjugated at various molar ratios, along with a gelling polymer under an ultraviolet (UV) lamp. Acryl group conjugation was confirmed through high performance liquid chromatography (HPLC) and mass …
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Photochemical Crosslinking Reactions in Polymers
… coatings, and the micro-patterning of surfaces. Photocrosslinking using additives is one of the safest and most robust methods as it allows precise control of the reaction in space and time.This thesis explores the photocrosslinking of polymers and relates it to structure and mechanism: the …
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Enzymology And Medicinal Chemistry Of N5-Carboxyaminoimidazole Ribonucleotide Synthetase : A Novel Antibacterial Target
… inhibitors, we developed a diazirine-based photocrosslinking agent to identify the binding site of these inhibitors. These studies revealed that the isatin core of class II inhibitors is capable of undergoing photochemical conversion to isatoic anhydride. Once formed, the anhydride is …
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Advancing Elastomers to Additive Manufacturing Through Tailored Photochemistry and Latex Design
… throughout the colloid. Incorporation of photocrosslinking chemistry into the continuous, aqueous phase of latex combined photocurability with the rheological advantages of latex and yielded a high molecular weight precursor suitable for VP. Continuous-phase photocrosslinking generated a …
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Synthese und Charakterisierung von Polymerbürsten-Oberflächen mit Gradienten und Strukturierung (Synthesis and characterization of patterned and gradient polymer brush surfaces)
… of the morphology of mixed polymer brushes via photocrosslinking by UV irradiation. Irradiated areas lose the ability to switch. Exposure to selective and non-selective solvent allows visualization or erasing of the written information, respectively. It is demonstrated that developed approaches …
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Biodegradable in situ forming photocrosslinked implants for sustained protein delivery to the posterior segment of the eye
… of formulation components, injection volumes and photocrosslinking duration, it was possible to modify release profiles significantly.<br/><br/>The implants studied were also capable of degrading via hydrolysis in biodegradation studies, showed high biocompatibility when tested against a retinal …
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The reaction kinetics and three-dimensional architecture of a catalytic RNA
… value of the model was.tested and confirmed by photocrosslinking experiments.
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Structure and Properties of Nano- and Meso-scale Materials Prepared by Designed Self-Aasembly of Nanoparticles
… process of gold nanorods by post-assembly photocrosslinking of polymer ligands. By functionalizing gold nanorods with a hydrophobic polymer containing pendant double bonds, we were able to compartmentalize a hydrophobic photoinitiator. We could then suppress the association ability of the …
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Development of cell-laden hydrogels with high mechanical strength for tissue engineering applications
… 6.9MPa. By using a cell-compatible two-step photocrosslinking process, it was also possible to encapsulate cells with high viability. Further research into the materials as tissue scaffolds showed that the DN hydrogels weakened when they were prepared at cell-compatible conditions, and …
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Composite hydrogel-nanofibre vessels for vascular graft applications
… fibres were fabricated by electrospinning and photocrosslinking techniques. The photocrosslinked PVA-SbQ fibre presented water-insoluble properties. Preliminary endothelial cell culture result showed evidence that the PVA-SbQ fibres could potentially act as a cell lining substrate. Finally, a …
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Chemical Tools for Exploring IFITM3 S-Palmitoylation and Mechanism
… new opportunities for live-cell imaging and photocrosslinking studies with specific interacting proteins in Chapter 4. My thesis studies have revealed additional insight into IFITM3 regulation by S-palmitoylation and established new tools to explore the antiviral mechanism of IFITMs.</p>
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Biomimetic Extracellular Matrices for In Vitro Cancer Models and Tissue Engineering Platforms
… alignment of CNC-MA was preserved by cooling and photocrosslinking of the extruded hydrogel sheets. The degree of structural anisotropy was controlled by varying the cooling temperature of the stage onto which the gel was extruded and the volumetric flow rate of the ink. The extruded hydrogel …
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Elucidating Cause-Effect Relationships between Extracellular Matrix Signaling and Mesenchymal Stem Cell Differentiation
… Scl2-2, and Scl2-3 were functionalized with photocrosslinking sites to enable incorporation into a 3D hydrogel matrix. characterization studies confirmed that the functionalization of the Scl2 proteins did not disrupt triple helix conformation, integrin binding, or cell adhesion. Also, …
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