Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 122 for “"photobleaching"”.
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Two -Photon Standing Wave Fluorescence Photobleaching With Application to the Study of Chromatin Motion
… fluorescence recovery after patterned photobleaching (2P-c-FRAPP) method is applied to the study of diffusive motion of chromatin in live cells and isolated nuclei. The chromatin is labeled using the DNA minor groove binding dye Hoechst 33342 or GFP labeled histone H2B. The diffusion is …
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Dynamics of DNA-repair factors and chromosomes studied by laser-UVA-microirradiation and laser-photobleaching
… regions were selectively marked by laser-photobleaching and followed by live cell microscopy. To this end, a live cell imaging system was established at a confocal laser-scanning microscope, which allows the observation of living cells for several days. Chromatin movements visualized by …
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The role of copper in aquatic photochemistry of dissolved organic matter
… to investigate the fundamental mechanism for the photobleaching and photooxidation of DOM from diverse sources in this thesis. In Chapter 2, the effect of nanomolar concentrations of Cu on 4-carboxybenzophenone (CBBP) and Suwannee River natural organic matter (SRNOM)-photosensitized degradation of …
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Using photo-instability to quantify fluorophores and achieve super-resolution imaging
… in biological imaging applications. Fluorophore photobleaching, blinking, binding, etc., is exploited to quantify and localize single fluorophores, even when their fluorescent images overlap those of nearby fluorophores. In the first technique, we image single, membrane-bound receptors labeled …
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Stimulated Emission Depletion (STED) Microscopy and Pacific Orange Dye Optimization For H9C2 Cox-1 Imaging Via Indirect Immunocytochemistry
… qualities of component materials, such as photobleaching of standard fluorophores. Current research aims to create smaller, cheaper STED models capable of using improved dyes that withstand photobleaching.</p> <p>The purpose of this study is to describe a simple method for standard …
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In Vivo Investigation of Protein Interactions in Caenorhabditis Elegans by Forster Resonance Energy Transfer Microscopy
… protein expression levels were observed, and photobleaching of CFP during the measurements had to be accounted for. The CFP photobleaching kinetics were also measured to quantify the FRET efficiency. At high signal-to-noise, different FRET microscopy methods were reliable. The measurements …
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The Role of Fluorescence and Human Factors in Quantitative Transdermal Blood and Tissue Analysis Using NIR Raman Spectroscopy
… in vivo human fingertip skin autofluorescence photobleaching under 200 mW 830 nm NIR irradiation is observed and it is characterized that: i) the majority of the photobleached fluorescence originates from static tissue not blood, ii) the bleaching (1/e point) occurs in 101-102 sec timescale, …
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Ultra-rapid 2-D and 3-D laser microprinting of proteins
… high-speed photochemistry based on multi-photon photobleaching of fluorescein, and we build the fastest 2-D and 3-D laser microprinter for proteins to date. First, we show that multiphoton photobleaching of a deoxygenated solution of biotin-4-fluorescein onto a PEG monolayer with acrylate …
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Dual-Colour STED-microscopy on the Nanoscale
… infinite resolution was limited due to photobleaching of the fluorescent species. In this thesis, dark-state relaxation (D-Rex) has been traced in a comprehensive study on one- and two-photon excitation to crucially reduce photobleaching in general thus leading to a major signal increase …
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Marsh-Exported Dissolved Organic Matter Fate in Estuaries
… while CDOM quality remained the same throughout. Photobleaching resulted in a net loss of CDOM and humic-like FDOM and a decrease in CDOM molecular weight, while microbial degradation resulted in a net loss, or no change, of CDOM absorption coefficients and a net increase in humic-like FDOM. …
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Optimization of photonic crystal enhanced fluorescence by excitation laser angle scanning
… crystal enhanced fluorescence in the context of photobleaching. We show that the photobleaching rate of dye molecules on the photonic crystal surface is accelerated by 30x compared to an ordinary glass surface, but substantial signal gain is still evident, even after extended periods of …
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Applications of Pump-Probe and Two-Photon Techniques in Time-Resolved Fluorescence Microscopy and Spectroscopy
… systems. Good spatial resolution, reduced photobleaching, and efficient rejection of excitation wavelength from emitted fluorescence are the main characteristics of this technique. As part of this work, extension of two-photon microscopy to time-resolved polarization imaging of probe …
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Fluorescence imaging microscopy studies on single molecule diffusion and photophysical dynamics
… time and high spot density, in combination with photobleaching) on the recovered diffusion coefficients were carefully studied. The performance of the method was demonstrated in two series of experiments. In one series, the diffusion of a fluorescent lipid probe in artificial lipid bilayer …
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Characterisation of Rhodopsin Retinitis Pigmentosa mutants located in Intradiscal Loop 1
… their UV-visible absorption spectral properties, photobleaching properties, active light-activated state stability kinetics and pigment thermal stability. Pharmacological chaperone rescue with 9-cis-retinal was found to enhance expression levels and folding in two of the three mutant pigments. …
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MiniFAST: A Fast and Sensitive Microscope for in vivo Neural Imaging
… which can eliminate autofluorescence and photobleaching that occurs in fluorescent indicators. Despite their potential, miniaturized microscopes are not in use with these indicators likely due to their inability to image at high speeds to capture the fast dynamics of GEVIs and inability to …
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Three-Dimensional Fluorescence Microscopy by Optical Scanning Holography
… 3D image acquisition time, resolution limits and photobleaching. The analysis shows that an optimized OSH-based fluorescence microscope can offer improved image acquisition time with equal lateral resolution, but with degraded longitudinal resolution when compared to confocal scanning optical …
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Design, synthesis and characterization of fluorescent hyperbranched polyglycerols and organic nanoparticles with covalently bound anti-fading agents
The photobleaching of fluorophores is a major limitation in fluorescence microscopy. We sought to create a brighter and more stable fluorophore by covalently attaching fluorescein and anti-fading agent (AFA) moieties to hyperbranched polyglycerols (HPG). The use of HPGs as a scaffold to link …
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Towards a carbon nanotube antibody sensor
… because of extraordinary sensitivity, lack of photobleaching, and optical activity in a near-infrared window. A SWNT sensor could provide label-free measurements of antibody concentration in a continuous fashion, which may aid selection of production strains. Protein A itself, dextran, poly …
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Investigating the assembly of axonal domains for saltatory conduction
… to the PS, I used Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Loss In Photobleaching (FLIP) on live DRG neuronal cultures. I found that Nfasc186 is delivered by bidirectional lateral diffusion from soma and distal axon to the PS of PNS DRG neurons, the same pathway …
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