Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"photo-cross-linking"”.
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Photo-cross-linking and identification of nuclear proteins that bind to DNA containing a site-specific adduct of cis-[Pt(NH₃)(N-6-aminohexyl)-4-benzophenonamide)CI₂]
… These challenges were addressed by using a photo-active cisplatin analogue, cis-[Pt(NH3)(N-(6-aminohexyl)-4-benzophenonamide)C12] (PtBP6). Site-specifically modified DNA probes were synthesized and used to identify proteins photo-cross-linked by the benzophenonemodified platinum complex. A …
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Towards the development of a visible light-enabled histidine-selective protein modification strategy using diazo sulfonium-based probes
… such as affinity/activity-based probes or photoaffinity labelling (PAL) are invaluable tools for the interrogation and study of biological processes in cellulo. Among those, PAL has proven to be the most powerful method, supplemented by the temporal control of its photoactivation step. …
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Mechanism and regulation of ribosomal RNA transcription
… using electrophoretic mobility shift and photo-cross-linking assays. Furthermore, we identify an interaction between a pol I specific subunit and a subunit of the fundamental pol I transcription factor. An interaction that may be important for the recruitment of pol I to the rRNA promoter. …
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New approaches for the synthesis of unusual amino acids
… so are promising targets for anti-viral drugs. Photoactivatable amino acids have been used to identify these interactions. Activation by ultraviolet light induces covalent cross-linking (known as photo cross-linking) of the interacting proteins which can be detected with high specificity by …
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Load bearing regenerative repairs for the cartilaginous soft tissue of the intervertebral disc
… co-continuous morphology. After photo-cross-linking, the elastomer phase contributed the majority of the initial stiffness to the co-continuous scaffolds. Hydrolysis of the elastomer phase, controlled the decrease in the co-continuous scaffold modulus from fast (2 – 3 weeks) to …
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Preparation and Biocompatibility of Electrospun Zwitterionic Poly(Sulfobetaine Methacrylate) for Wound Dressing Applications
… would readily dissolve in water, whereas if crosslinker is added, the formation of hydrogel would prevent electrospinning. A three step polymerization-electrospinning-photo-cross-linking process was developed in this work to fabricate the cross-linked electrospun PSBMA fibrous membrane. Such …
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Functional Triblock Terpolymers for Multicompartment Micelle and Janus Particle Synthesis
… terpolymer bulk structures via selective cross-linking of the middle block. Until now such soft JPs were prepared mainly from polystyrene-block-polybutadiene-block-poly(methyl methacrylate) and polystyrene-block-polybutadiene-block-poly(tert-butyl methacrylate). However, these polymers do …
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Application of Genetically-Encoded Photoactivatable Crosslinkers to Map Ligand-Binding Sites on G Protein-Coupled Receptors
… in the human genome. A general cellbased photocrosslinking approach was developed to investigate the binding interfaces necessary for the formation of GPCR signaling complexes. Amber codon suppression was extended to facilitate the incorporation of photoactivatable unnatural amino acids, …
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Approaches to Study Small Molecule Inhibitors and Their Targets
… called Stable Isotope Labeled Inhibitors for Crosslinking (SILIC), structure-activity relationship data is used to design inhibitor analogs that incorporate a photo-crosslinking group along with either natural or heavy stable isotopes. An equimolar mixture of these inhibitor analogs is …