Global ETD Search
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Showing 1 to 20 of 51 for “"phosphotyrosine"”.
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Molecular Cloning of Avian Sarcoma Virus CT10 and Characterization of its Protein Product
… discussed. Biochemical data demonstrated that phosphotyrosine levels on at least three cellular proteins were greatly elevated in CTI0-infected cells, and that a tyrosine kinase activity was immunoprecipitated in association with p47<sup>gag-crk</sup>. A specific antiserum that recognizes the …
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Bridging the gap between protein-tyrosine phosphorylation networks, metabolism and physiology in liver-specific PTP1b deletion mice
… was used to quantitatively discover protein phosphotyrosine network changes in L-PTP lb-/- mice relative to control mice under both normal and high-fat diet conditions. A phosphosite set enrichment analysis was developed to identify numerous pathways exhibiting PTPlb- and diet-dependent …
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Identification of a Low Molecular Weight Protein Tyrosine Phosphatase and Its Potential Physiological Substrates in Synechocystis sp. PCC 6803
… phosphate (p-NPP), beta-napthyl phosphate and phosphotyrosine but not toward alpha-napthyl phosphate, phosphoserine, or phosphothreonine. Kinetic analysis indicated that the Km (0.6 mM) and Vmax (3.2 mmole/min/mg) values for SynPTP toward pNPP are similar to those of other known bacterial low …
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Protein/Peptide Recognition Modules in Cellular Signaling and HIV Pathogenesis
… domains: SH2 (Src homology 2) domains, PTB (phosphotyrosine binding) domains, and SH3 (Src homology 3) domains. The SH2 domains recognize tyrosylphosphorylated peptides using a binding mode that is conserved among the SH2 family members. In particular, the structure of the SH-PTP2 SH2 domain …
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Vergleich der Signaltransduktion von Interleukin-6 und Interleukin-10 in primären humanen Makrophagen
… previously shown to recruit SOCS3 to one of its phosphotyrosine residues (Y759), sequence comparison of phosphotyrosine motifs suggests that the SH2-domain of SOCS3 does not interact with phosphorylated tyrosine motifs of the IL-10R. Taken together, different sensitivities of IL-10 and IL-6 …
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Growth regulation in the protozoan parasite Trypanosoma brucei: Evidence of tyrosine-specific protein kinase activity
… trypanosome protein revealed the presence of phosphotyrosine. An immunoblot of trypanosome proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and incubated with an anti-phosphotyrosine monoclonal antibody revealed the presence of a tyrosine phosphoprotein. An …
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Frazzled And Abelson Interact To Regulate The Actin Cytoskeleton In Drosophila
… filopodia, and is associated with an increase in phosphotyrosine levels as well as alterations in localization of proteins modulating actin dynamics and also known to interact with Abelson. Loss of Abl by RNA interference causes S2 cells to form peripheral microspikes as well as a decrease in …
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The role of integrin mediated adhesion and signaling during Xenopus retinal development
… beta1 integrin, talin, vinculin, paxillin and phosphotyrosine in retinal glial cell line XR1 and in the developing Xenopus retina. These proteins are colocalized at focal adhesions located at the termini of F-actin filaments in XR1 cells. These proteins display spatial and temporal expression …
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Quantitative approaches to probe the acetylproteome
… that short-term HDAC inhibition alters phosphotyrosine-signaling networks. To better understand this behavior, a suite of biochemical and computational methods were developed. Bromodomains were engineered to explore binding preferences using degenerate peptide arrays as well as develop …
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Mechanism of TNF-α cytotoxicity in a leukemia virus transformation model
… was determined by a radio-labelled anti-phosphotyrosine antibody in an antigen capture assay. TNF-α had little effect on p120 phosphotyrosine levels of TNF-α insensitive CL.7 and 3R.1 clones. The, TNF-α sensitive, 6R.1 clone, however, was found to induce its p120 specific phosphotyrosine …
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Exploring the Regulation of Mitotic PP2A-Rts1 Activity in Saccharomyces cerevisiae
… PP2A phosphatase activator (PTPA), PP2A gains phosphotyrosine specificity. My work indicates that Rrd1 and Rrd2 (the budding yeast homologs of PTPA) genetically interact with PP2ARts1 and the absence of these proteins cause a Swe1-dependent delay in mitosis. In parallel, utilizing a candidate …
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Mechanistic Investigation of Genotype-Phenotype Correlations in PIK3R1-Related Diseases
… p85$\alpha$ mutations impaired phosphotyrosine peptide-stimulated PI3K activity when heterodimerised with either of the Class IA catalytic subunit isoforms. Two cancer-associated mutations assessed using the same assay demonstrated differential effects on PI3K function, causing …
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DNA catalysts as phosphatases and as phosphoserine lyases
… exhibited multiple-turnover activity with phosphotyrosine-containing free peptides and were active even in the presence of externally added cell lysate or bovine serum albumin (BSA). Furthermore, the best DNA phosphatase functioned with a larger protein substrate. This established the …
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The intramolecular domain interactions and phosphatase activation mechanisms of Shp2
… via intramolecular interaction between C-SH2 and phosphotyrosine 580 (pY580). The phosphotyrosine 542 (pY542) has an adverse position despite a favored surrounding amino acid sequence such that it can only bind to C-SH2 in the absence of pY580. In contrast, pY542 dominates the binding of C-SH2 in …
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Funktion der Protein-Tyrosin-Phosphatase SHP2 bei der Interleukin 6 vermittelten Akutphase - Genregulation und T-Zell-Migration
… Interestingly, one SHP2-recruiting phosphotyrosine motif in an single chain of the gp130 dimer is sufficient to mediate SHP2 association to the gp130 receptor subunit and its tyrosine phosphosylation as well as to attenuate IL-6-dependent gene induction. Furthermore, it was shown …
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Hemodynamic regulation of blood-brain barrier phenotype: A cooperative effort linking adherens and tight junction complexes
… border localization of ZO-1 and reduction in phosphotyrosine (pTyr)-occludin levels induced by high shear.
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Sulfatides mediate Disabled-2 membrane localization and stability during platelet aggregation
… Dab2 inhibits platelet aggregation through its phosphotyrosine-binding (PTB) domain by competing with fibrinogen for ï ¡IIï ¢3 integrin binding on the activated platelet surface.3 Sulfatides are also found on the platelet surface,4 interacting with adhesive and coagulation proteins5-7 and, thus, …
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Membrane binding properties of Disabled-2
… aggregation. During endocytosis, the Dab2 phosphotyrosine-binding (PTB) domain mediates protein binding to phosphatidylinositol 4,5-bisphosphate (PIP2) at the inner leaflet of the plasma membrane and helps co-localization with clathrin coats. Dab2, released from platelet alpha granules, …
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Untersuchungen zum crosstalk zwischen Interleukin-6 und Platelet-derived growth factor (PDGF) sowie zur Signaltransduktion von PDGF-Rezeptoren
… preferences for a binding of SOCS3 to the phosphotyrosine motives pY1009 and pY1021. The amino acid sequence V-L-pY-T-A-V-Q-P of the phosphotyrosine motive pY1009 matches the consensus sequence h-X-pY-h/S/T-XL/V-h-h predicted by Hörtner et al. for the binding of the SH2 domain of SOCS3. …
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Metal-protein interactions for proteomics applications
… discuss a study to couple PolyMAC-Ti with anti-phosphotyrosine antibody to investigate Syk and Lyn dependent phosphorylation in B-cells. In chapter 4, we introduce a method to capture and fractionate proteins based on copper-protein interactions. Its unique application in enriching and …
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