Global ETD Search
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Showing 1 to 9 of 9 for “"phosphotriesterase"”.
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Development of a phosphotriesterase enzyme assay for determination of organophosphate pesticides
Phosphotriesterase catalyzes the hydrolysis of a wide range of organophosphate insecticides such as paraoxon and parathion and nerve toxins such as soman and sarin. This project resulted in the development of novel phosphotriesterase enzyme assays to measure paraoxon and other representatives of …
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Methods and applications in computational protein design
… on a subset of degradation-prone amino acids in phosphotriesterase, an enzyme that hydrolyzes toxic organophosphates including pesticides and chemical warfare agents. Second, we introduce BroMAP/A*, an exhaustive branch-and- bound search technique with enumeration. We compare performance of …
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Exploring the emergence of pesticide degradation in the α/β hydrolase superfamily
… screening and displays weak promiscuous phosphotriesterase (PTE) activity. P91’s PTE activity is remarkable because, in contrast to other currently known PTEs, it utilises a fundamentally different, metal-independent mechanism. At the same time, it has the same fold and a very similar …
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Soil phosphatases: factors affecting enzyme activity in Arctic tussock tundra and Virginia mineral soils
… 10 fold greater than either phosphodiesterase or phosphotriesterase activity in the same soil. Three hundred to 700 µg p-nitrophenol ·g⁻¹ h⁻¹ were released by Arctic soils incubated at 0°C; levels increased as temperature increased. K<sub>m<sub>app</sub></sub> (v: v/S regression analysis) ranged …
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Characterising fitness landscapes of protein evolution by next-generation sequencing
… InDels and substitutions in three systems: GFP, phosphotriesterase (PTE) and the kinase MKK1 docking domain. Towards this goal, I established two experimental assays (GFP, PTE) for deep mutational scanning and a new software toolkit, InDelScanner, for interpreting resulting data that contain …
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Enzyme and Directed Evolution Technologies For Nerve Agent Neutralisation
… characterised are <i>Pseudomonas diminuta</i> phosphotriesterase (PTE) and PON1, a mammalian member of the Serum paraoxonase (PONs) family. These enzymes have excellent catalytic properties towards some OPs, but relatively poor activities against others. It has been possible to alter PTE …
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High-throughput protein engineering tools for efficient exploration and quantitative mapping of protein fitness landscapes
… measurements of protein variant’s fitness. Using phosphotriesterase as an example, I describe the repurposing of previously developed polyacrylamide bead display strategy for enzyme screening and the development of individual platform modules: on-bead DNA library synthesis, emulsion-based in vitro …