Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"phosphorylated proteins"”.
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Exploring Zirconia as a Column Packing Material
… can be used for the separation and analysis of phosphorylated proteins. Adenosine triphosphate, Adenosine diphosphate and Adenosine monophosphate were used as prototypes for phosphorylated proteins. Separation, absorption, fluorescence and SEM studies were performed to determine the adsorption …
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Characterization of IphP from Nostoc commune UTEX 584 and a Dual Specificity Protein Phosphatase from Anabaena PCC 7120
… of histidine and aspartate residues of proteins, while "eukaryotic phosphorylation" was characterized by modification of serine, threonine, or tyrosine residues. Increasing numbers of reports have emerged challenging the traditional view of "prokaryotic" and "eukaryotic" phosphorlyation. …
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Characterisation of protein phosphorylation in Arabidopsis thaliana and Chlamydomonas reinhardtii based on affinity chromatography and mass spectrometry
… of life. However, the low abundance of phosphorylated proteins hinders investigations following this direction. To circumvent this problem a new method for the enrichment of phosphorylated proteins out of complex protein mixtures was developed. This method relies on the affinity of the …
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Identification and characterization of diatom kinases catalyzing the phosphorylation of biomineral forming proteins
… (silica). Long-chain polyamines and highly phosphorylated proteins, silaffins and silacidins, are believed to play an important role in biosilica formation. The phosphate moieties on silaffins and silacidins play a significant role in biomineral formation, yet no kinase has been identified …
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Mechanistic Characterization of RTK Signaling Networks Using Phosphoproteomic Approaches
… over the past few decades to allow analysis of phosphorylated proteins with increased sensitivity and accuracy. Using these methods in combination with a systematic mutational strategy that evaluates the network effects of loss of phosphorylatable tyrosines that the protein of interest seemingly …
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Effects of Polyphenols on Vasomodulatory Factors and Associated Cell Signalling
… to assess their effect on a broad range of phosphorylated proteins using an antibody microarray. Quercetin (50 μM) significantly decreased eNOS phosphorylation (p < 0.05), while extracts of pomegranate and persimmon significantly increased levels of phosphorylated (p-) Akt, p-eNOS and cell …
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The Mycobacterium tuberculosis protein O-phosphorylation landscape
… a larger repertoire of both STPKs and O-phosphoproteins than most bacteria, suggesting a more prevalent role of STPKs in Mtb. Many studies have identified individual STPK functions and substrates, but a systems-level understanding and the full scope of O-phosphorylation in bacteria in general …
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B Cell Activation: The Regulation by T Cells and the Induction of the Protein Tyrosine Kinase SYK
… is required for the accumulation of tyrosine phosphorylated proteins and for the induction of in vitro phosphotransferase activity of at least one kinase. The protein tyrosine kinase syk is one of the major endogenous substrates of this kinase activity. Moreover, we demonstrated that the in …
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The role of adaptor proteins Crk and CrkL in lens development
… elongation have not been identified. Adaptor proteins Crk and CrkL are SH2- and SH3-containing proteins that transduce signals from upstream tyrosine phosphorylated proteins to downstream effectors, including Ras, Rac1 and Rap1, which are important for cell proliferation, adhesion and …
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Elucidating the role of the EGFR-family members in cell motility through the use of novel engineered bivalent ligands
… the relationship between the measured signals of phosphorylated proteins and the cellular migration phenotypes, seeking novel potential therapeutic targets. Overall, this work illustrates the complexity of the EGFR signaling pathway and the need for new paradigms to target its signaling pathway to …
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The role of the BARD1 BRCT domains in the DNA damage response
… its C-terminus which are important for binding phosphorylated proteins involved in DNA repair. Mutations in the BARD1 BRCT domains have been discovered in a few breast and ovarian cancers which suggested that BARD1 is important in the DNA damage response (DDR) independent of its function in …
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Proteomic studies of the pheromone biosynthetic pathway in the bark beetle, Ips pini, and molecular modeling of an insect-specific aldehyde oxidative decarbonylase, CYP4G2
… staining were used to examine the expression of proteins and phosphorylated proteins involved in the pheromone producing midgut tissue. The results show a lack of a linear relationship between the high transcript levels previously reported and an increase in protein expression. In addition, …
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Acetaldehyde and Epithelial Junctions: Role of Pp2A and Occludin Phosphorylation
… in the phosphorylation of tight junction proteins. It also reacts with proteins, lipids, and DNA to cause cellular malfunction.</p> <p>This project explored the roles of PP2A (a protein phosphatase) in acetaldehyde-mediated tight junction disruption, wound healing, and morphological …
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The role of protein phosphorylation in the switching between modes of synaptic vesicle release
… vesicle exocytosis, but did not reveal which proteins might be involved in directly modulating the properties of the fusion pore involved in exocytosis. Investigations were carried out to see if dynamins or myosin 2 could regulate the fusion pores of synaptic vesicles since these proteins have …
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Tumor cell-intrinsic signals promoting tolerance and adaptation to oncogenic kinase inhibition
… mass spectrometry (MS) of endogenous tyrosine-phosphorylated proteins, which enables phosphosite-specific quantification of TK signaling networks, I report several cell line-specific vulnerabilities not predicted to exist at the genetic level, and the consensus observation that sustained …
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Phosphoproteins of the Cell Nucleus: Metabolism and Characterization
… with the chemistry and metabolism of the phosphorylated proteins which are found localized in cell nuclei. The studies focus first on phosphoprotein metabolism as it occurs in the intact nucleus under varying conditions, and then on the chemistry and enzymology of a phosphoprotein fraction …
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Understanding grapevine responses to abscisic acid
… isobaric-label method. Each identified common proteins, but also additional proteins not found with the other method. Overall, several thousand proteins were identified and several hundred were quantified. In addition, hundreds of phosphoproteins were identified. Tens of proteins were found to …
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Multi-omic Studies of Neutrophils Reveal Programmed Deactivation Phenotypes After Inflammatory Stimulus
… Within the enriched pathways associated with phosphorylated proteins, phagocytosis and degranulation were prevalent, while mRNA processing emerged as the consensus term among dephosphorylated proteins. These findings were corroborated at the kinase level, where active kinases were …
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The Relevance of the SIRP Protein Family to Signal Transduction and Cell Adhesion
… SIRPs are a recently discovered family of glycoproteins comprising more than 30 members belonging to the immunoglobulin superfamily. The two different structural subtypes, termed SIRP α and SIRP β, are distinguished by the presence or absence of a cytoplasmic domain, respectively. SIRP α1, the …