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Showing 1 to 20 of 68 for “"phosphorylase"”.
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Mechanistic studies of rabbit muscle glycogen phosphorylase
Glycogen phosphorylase catalyzes the reversible phosphorolysis of glycogen. The enzyme contains a molecule of pyridoxal phosphate (PLP), of which the phosphate moiety is essential for catalysis. To investigate the role of the cofactor phosphate moiety two phosphonate analogues of PLP, …
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Regulation of glycogen phosphorylase genes in Dictyostelium discoideum
… Glycogen degradation is catalyzed by glycogen phosphorylase, and two developmentally regulated glycogen phosphorylase activities have been discovered in Dictyostelium. Glycogen phosphorylase 1 (gp-1) activity is predominant early in development, and is dependent upon 5’ AMP as a positive …
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Analyzing the functions of human polynucleotide phosphorylase (hPNPaseold-35)
… conserved enzyme is polynucleotide phosphorylase (PNPase). The human homologue of this fascinating enzymatic protein (hPNPaseold-35) was cloned a decade ago in the context of terminal differentiation and senescence through a novel ‘overlapping pathway screening’ approach. Since then, …
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Molecular mechanism of glycogen phosphorylase gene regulation during Dictyostelium development
… and especially gene regulation. Glycogen phosphorylase is the enzyme that initiates one of the key biochemical pathways, glycogen degradation, during Dictyostelium discoideum development. Two forms of glycogen phosphorylase, gpl and gp2, exist in D. discoideum with gp1 being active in …
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Identification of Phosphorylase Kinase Alpha Subunit Binding Partners in Skeletal Muscle
<p>IDENTIFICATION OF PHOSPHORYLASE KINASE ALPHA SUBUNIT BINDING PARTNERS IN SKELETAL MUSCLE Soleil Archila August 2004 67 Pages Directed by: Nancy A. Rice, Sigrid Jacobshagen, and Claire A. Rinehart Department of Biology Western Kentucky University Phosphorylase kinase (PhK) integrates neural, …
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The relationship between two forms of glycogen phosphorylase in Dictyostelium discoideum
… responsible for glycogen degradation, glycogen phosphorylase, exists in active and inactive forms. The activity of the ’a' form (the active form) is independent of 5′adenosine monophosphate (5′AMP) while the activity of the ’b' form (the inactive form) is 5′AMP dependent, The two forms are …
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The relationship between the two forms of glycogen phosphorylase in Dictyostelium discoideum
… regulated forms of the enzyme glycogen phosphorylase. The inactive ’b’ form requires 5'AMP for activity and is present in early development, whereas the active ’a’ form is 5'AMP independent and is present in late development. Polyclonal antibodies raised to purified forms of the enzyme …
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Computer-aided design and synthesis of 5,7-dihydroxyflavone derivatives as glycogen phosphorylase inhibitors
… flavonoids are known inhibitors of glycogen phosphorylase, which is a validated target for controlling hyperglycemia in type 2 diabetes. By exploiting computational methods such as molecular docking with Glide and post-docking binding free energy calculations using advanced QM/MM-PBSA …
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Baculovirus-directed expression of the phosphorylase kinase catalytic subunit: pseudosubstrate and calmodulin regulation
Phosphorylase kinase (EC 2.7.1.38) is a key enzyme involved in the regulation of the glycogenolysis pathway. It catalyzes the Ca²⁺-dependent phosphorylation and activation of the enzyme glycogen phosphorylase to make the active form glycogen phosphorylase. Phosphorylase kinase is composed of 4 …
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Transcriptional Regulation of the Glycogen Phosphorylase-2 Gene in <I>Dictyostelium discoideum</i>
The expression of the <I>glycogen phosphorylase- 2</I> gene (<I>gp2</I>) is initiated during early development and regulated by the extracellular morphogens cAMP and Differentiation Inducing Factor (DIF-1) [1-3]. Glycogen phosphorylase- 2 catalyzes the breakdown of glycogen reserves in developing …
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Footprint Analysis of the Transcriptional Control of Glycogen Phosphorylase 2 in Dictyostelium Discoideum
Glycogen phosphorylase 2 (gp-2) is a key enzyme during the development of Dictyostelium discoideum. The gp-2 enzyme breaks down glycogen into glucose monomers that are subsequently used to synthesize the terminal end products of cellular differentiation. This gene is an ideal candidate for studying …
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An Investigation of Factors Affecting the Pyridoxine Requirement of Postpubertal Gilts (Metabolism, Phosphorylase, Muscle)
Various nutritional factors were investigated with respect to their effect on the dietary B6 requirement of swine. Factors investigated included the extent and significance of B6 storage in muscle tissue, the effect of a riboflavin deficiency on B6 metabolism and thus on B6 status, and finally the …
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Molecular analysis of glycogen phosphorylase-1 gene expression during the development of dictyostelium discoideum
… regulated forms of the enzyme glycogen phosphorylase, which are encoded by two distinct, but related genes (Rutherford, et. aI., 1991). A complementary DNA (cDNA) encoding glycogen phosphorylase-}, gp-l, was isolated from a λgtll expression library made from amoebae stage mRNA. The 5' …
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Molecular characterisation of XvVTC2, a gene coding for a GDP-L-galactose phosphorylase from Xerophyta viscosa
Climate change is predicted to have a negative impact on world food security in the next 40 years. Resurrection plants can withstand highly variable and harsh climatic conditions. This makes them ideal candidates to elucidate possible mechanisms which can be used to adapt crop plants to tolerate …
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A study of the regulation of glycogen metabolism in Dictyostelium discoideum
… possible cAMP dependent regulation of glycogen phosphorylase and glycogen synthase was examined. In other systems, cAMP can regulate at the level of the gene (procaryotes, CAP protein) or at the level of covalent, reversible modification of the enzyme activity (eucaryotes, cAMP-dependent protein …
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(alpha)-Glucan-Phosphorylasen aus Weizen (Triticum aestivum L.) Isolierung, Charakterisierung und Analyse der Expression
Pflanzliche a-Glucan-Phosphorylasen (EC 2.4.1.1) können Stärke sowohl synthetisieren als auch abbauen. In Blättern und Körnern des Weizen var. Star können drei Phosphorylaseformen (P1, P2 und P3) mittels nicht-denaturierender Elektrophorese im Glycogen-haltigen Polyacrylamidgel unterschieden …
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