Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"phosphoproteome"”.
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Quantitative mass spectrometry-based approaches for characterizing the immunopeptidome and tyrosine phosphoproteome in cancer
Significant advancements in proteome-based analyses stem from innovations in the field of mass spectrometry (MS), an analytical method which allows for the sequencing, identification, and quantification of peptides and proteins in complex biological mixtures. MS enables a molecular and systems-wide …
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The identification of the mTOR-regulated phosphoproteome and a mediator of feedback inhibition to P13K-Akt
… this deficit, we defined the mTOR-regulated phosphoproteome by quantitative mass spectrometry and characterized the primary sequence motif specificity of mTOR using positional scanning peptide libraries. We found that the phosphorylation response to insulin is largely mTOR-dependent and that …
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In-depth bioinformatics analysis of the phosphoproteome of triple negative breast cancer treated with a tumor selective NQO1 bioactivatable drug
The main focus of this study is to elucidate changes in the proteome of triple negative breast cancer cells in response to a novel bioactivated anti-cancer agent IB-DNQ (isobutyl-deoxyniboquinone). NQO1 or NADPH:quinone oxidoreductase-1 is a detoxifying enzyme overexpressed in many solid tumors and …
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Translational phosphoproteomics methods to identify biomarkers and novel therapeutic targets
… interventions. Additionally, analysis of phosphoproteome in response to drug candidates can help identify biomarkers for therapeutic response as well as lend direct insights into potential adaptive resistance mechanisms. However, quantification of phosphoproteome, especially the …
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The Mycobacterium tuberculosis protein O-phosphorylation landscape
… phosphoproteomics. We found that the Mtb phosphoproteome is 5x larger than was previously reported. By identifying hypo-phosphorylated proteins in the LoF mutants and hyper-phosphorylated protein in the GoF mutants, we comprehensively identified over 1,400 STPK-substrates in the bacterium, …
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Unravelling the roles of the Mck1 kinase in metabolic reprogramming and the stress response
… 5), integrative analysis of the proteome and phosphoproteome was conducted with the aim to identify the targets of Mck1 that are implicated in metabolic reprogramming and the stress response. Many known and potential targets were revealed. Subsequent genetic and phenotypic assays indicated …
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Phosphoproteomics analysis of Alzheimer's disease
… I use mass spectrometry to characterize the phosphoproteome of post-mortem brain tissue from AD patients and multiple mouse models of AD. Using computational analysis, I identified several signaling pathways that are dysregulated before neurodegeneration occurs. Many of these signaling …
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Serine/threonine phosphorylation in mycobacterium tuberculosis : identification of protein kinase B (PknB) substrates
… of the signal transduction pathways and phosphoproteome in Mycobacterium tuberculosis.
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Proteomic Analysis Delineates the Signaling Networks of Plasmodium falciparum
… kinase PfPK7 as a model. Our comparison of the phosphoproteome profiles between the wild-type 3D7 and PfPK7- parasites identified 146 proteins with 239 phosphorylation sites exhibiting decreased phosphorylation in the absence of PfPK7 at the developmental stages where nuclear division and …
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Clarification of the xanthan biosynthesis mechanism in Xanthomonas campestris pv. campestris B100 by mass spectrometry-based proteome analysis
… In addition to the proteome analysis, a phosphoproteome analysis was conducted to further explore the "methionine effect." The method was adapted to accommodate increased culture volumes, and a phenol solution called TRIzol was used to extract sufficient protein for phosphopeptide …
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Multi-omic Studies of Neutrophils Reveal Programmed Deactivation Phenotypes After Inflammatory Stimulus
… employed in this investigation include proteome, phosphoproteome, kinome, and transcriptome profiling. Data interpretation was facilitated through clustering and pathway enrichment analysis. This multi-omic approach was complemented by a multi-parametric assay of neutrophil functions. Analysis of …
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Investigating the dynamic nature of biomolecular condensates that support rotavirus replication
… comparative analysis of host proteome and phosphoproteome following infection with either the low LLPS propensity variant or the wild-type RVA indicates that viroplasms may facilitate immune evasion and modulate various host cellular processes. Therefore, my results suggest additional roles …
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Molecular Characterisation of the Brassinosteroid, Phytosulfokine and cGMP-dependent Responses in Arabidopsis thaliana
… we note that this can affect changes in the phosphoproteome in Arabidopsis and conclude therefore that the cGMP plays a role in kinase-dependent downstream signalling. The obtained results suggest that the receptor molecules investigated here belong to a novel class of GCs that contains both …
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Enabling tools for biological analysis : technologies for the study of protein dynamics, detection and interaction
… platform we were able to further probe the phosphoproteome for proteins that have multiple post-translational modifications. The widespread use of protein, DNA and small molecule microarrays has been limited in clinical and diagnostic settings due to the cost of microarray readers. …
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The role of JAK1 and JAK3 in CD8<sup>+</sup> effector T cells
… sites, with less than 1.2% of the CTL phosphoproteome significantly regulated by tofacitinib treatment following 4hrs tofacitinib treatment. Proteins with downregulated phosphorylation sites were enriched in functions related to the Jak-STAT signalling, regulation of gene expression, …
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Investigation of glycolysis in Bacillus subtilis
… Mehrfach- Kinasemutanten konstruiert und deren Phosphoproteome untersucht. Jedoch zeigten diese Mutanten keine Unterschiede im Phosphoproteom, verglichen mit dem Wildtyp. Deshalb wurde angenommen, dass unbekannte Kinasen oder andere Mechanismen der Phosphorylierung existieren. In dieser Arbeit …
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The C. trachomatis effector protein TepP hijacks host cell signaling pathways to promote bacterial survival during infection.
… cell cytoskeleton, modification of the host cell phosphoproteome, bacterial replication, and interferon-dependent gene activation. We have additionally determined that TepP interacts with the Crk family of host cell adaptor proteins, and the class 1 phosphoinositol-3-kinase (PI3K). Cell lines …
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Regulatory mechanisms of MYO6 motor domain activity and tail interactions
… of interactions. First, I examined the MYO6 phosphoproteome under steady state and stimulated conditions using mass spectrometry. After identifying multiple sites of interest, the S267 site is characterised *in vitro* and *in vivo*. This reveals that S267 phosphorylation increases the motor …
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The evolution of protein kinase specificity
… in a taxonomically broad range of species using phosphoproteome data. I then related the evolution of substrate motif sequences to that of their cognate effector kinases where possible. The results strongly suggest that many of the motifs emerged in a universal eukaryotic ancestor. I finish by …
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Identifying Common Therapeutic Targets in Merlin-deficient Brain Tumours
… body of knowledge regarding the proteome and phosphoproteome of meningioma and schwannoma. We generated a comprehensive set of data that highlighted several potential therapeutic targets and dysregulated pathways which will be further investigated.
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