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Showing 1 to 20 of 79 for “"phosphoprotein"”.
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Analysis of the functions of phosphoprotein phosphatase 1 catalytic subunits during cell division
… of phosphatases in the eukaryotic cell is the phosphoprotein phosphatase 1 (PP1) family of phosphatases. These phosphatases are holoenzymes composed of one of three catalytic subunits -α, β, and γ - in complex with one or two regulatory subunits. There is some evidence indicating that the three …
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Bedeutung des "vasodilator-stimulated phosphoprotein" (VASP) für die Entwicklung des Hörvermögens von Säugetieren - eine experimentelle Studie an Mäusen -
Das „vasodilator-stimulated phosphorprotein“ (VASP), ein Mitglied der Ena/VASP Proteinfamilie, gilt als ein entscheidender Faktor bei der Regulation von Aktindynamik. Es ist an Zellbeweglichkeit und –adhäsion, unter anderem in Filopodien und dem Wachstumskegel auswachsender Neurone beteiligt. …
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Phosphoproteins of the Cell Nucleus: Metabolism and Characterization
… in cell nuclei. The studies focus first on phosphoprotein metabolism as it occurs in the intact nucleus under varying conditions, and then on the chemistry and enzymology of a phosphoprotein fraction which has been extracted and purified from isolated nuclei. The metabolism of …
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Protein Phosphorylation in Archaea
… was initiated to characterize the most prominent phosphoproteins in S. solfataricus. Cell extracts were incubated with [γ-³²P] ATP, MgCl₂, and MnCl₂, and the proteins in the extracts were separated by SDS-PAGE. Autoradiography of the gels revealed four prominent phosphoproteins with apparent …
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Purification and Activity of the PP60SRC Protein Kinase
… from the action of a single virally coded phosphoprotein with a molecular weight of 60,000 daltons (pp60<sup>src</sup>). The expression of pp60<sup>src</sup> results in many significant changes in the cell's phenotype which include disruption of the cytoskeleton and the deregulation of cell …
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A Species Comparative Study of the Soluble Phosphoproteins of Dentin.
The soluble phosphoproteins of the dentin have been isolated from the rabbit, cow, hog, and human. The phosphoproteins were purified by means of gel filtration and anion-exchange chromatography, and polyacrylamide gel electrophoresis. They all showed high levels of aspartic acid, serine, and …
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Investigation of Vesicular Stomatitis Virus Interaction with Host Cell Proteins and Associated mRNA Structures
… glycoprotein mRNA, and between hnRNP A1 and VSV phosphoprotein mRNA. Additionally, a number of RNA-RNA and RNA-protein binding motifs were identified in VSV nucleoprotein, phosphoprotein and glycoprotein mRNA.
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Study on polypeptides of cyanobacterial photosystem 2
… for small polypeptides of 8, 10 and 12kDa. A phosphoprotein of 14kDa was shown to be related to the psb H gene product and a phosphoprotein of approximately 32kDa, possibly Dl, was also obtained.A further 58kDa phosphoprotein which may be related to the calmodulin site or to the protein kinase …
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Application of Different Bioanalytical Workflows for Proteomics of Prostate Cancer
… in prostate cancer </p> <p>Our study in phosphoprotein signatures on a large scale in prostate cancer focused on the LNCaP human prostate cancer cell line, and on human prostate cancer tissue. </p> <p>For the LNCaP prostate cancer cell line, we applied a combination of analytical …
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Expanding the toolbox for precision medicine with silicon photonic microring resonators and microfluidic technologies
… summarized as the implementation of protein and phosphoprotein detection in whole cell lysates and tissue homogenates. Protein detection in cell lysate was achieved by modifying the signaling amplification schemes developed by previous lab members and altering the chemical strategy for covalent …
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Gammabestrahlung führt zur CD95-abhängiger Invasivität in apoptoseresistenten Glioblastomzellen
… in dieser Studie nicht detektiert werden. Das „phosphoprotein enriched in diabetes/phosphoprotein enriched in astrocytes-15-kDalton“ (PED/PEA-15) wurde als möglicher Kandidat untersucht. Jedoch konnte nach dem Ausschalten der PED/PEA-15-Expression keine Veränderung in der Inhibierung von GSK3 …
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AUTOANTIGENS IN PARKINSON'S DISEASE
… α-synuclein and the stress inducible phosphoprotein 1(STIP1) in PD. α-synuclein has been widely implicated in PD pathogenesis while STIP1’s role in PD remains to be determined. Here, we performed a detailed characterization of autoantibodies and antigen-specific T cells using an …
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Structural characterization of the C-terminal domain of human nucleophosmin, NPM1
<P>NPM1 IS A MULTIFUNCTIONAL PHOSPHOPROTEIN WHICH SHUTTLES BETWEEN NUCLEUS AND CYTOPLASM. WHEN NPM1 INTERACTS WITH P53, A MUTATION IN ITS C-TERMINUS LEADS TO SEQUESTRATION OF P53 INTO CYTOPLASM, PREVENTING IT FROM GOING INTO THE NUCLEUS. WE HAVE CARRIED OUT STRUCTURAL STUDIES ON THE 53 RESIDUE …
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Interaktion des Proteins ActA von Listeria monocytogenes mit dem Wirtszellprotein LaXp180
… die direkt mit ActA interagieren, sind das Phosphoprotein VASP und der Arp2/3-Komplex. VASP bindet an den zentralen prolinreichen Bereich von ActA und beschleunigt durch die Rekrutierung von Profilin den Prozeß der Aktinpolymerisation. Der Arp2/3-Komplex interagiert mit dem N-terminalen …
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Characterization of the phosphorylation of thylakoid membrane proteins from cyanobacterium synechococcus sp. PCC 6301 in light state 1 and light state 2
… of intact cells in state 1 and state 2. Phosphoproteins were identified by gel electrophoresis of both thylakoid membrane and phycobilisome fractions isolated from cells labelled with 32p orthophosphate. The results showed very close phosphoprotein patterns for either thylakoid membrane …
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Characterization of Tuba, a novel Ena/VASP ligand, and function of Ena/VASP proteins in mouse development
… Enabled), VASP (Vasodilator Stimulated Phosphoprotein), and EVL (Ena-VASP-Like). This thesis work focused on understanding the vertebrate EnaNASP protein family by discovering pathways that regulate EnaNASP function and by defining the role of EnaNASP proteins in vertebrate development. …
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The effect of dietary zinc level upon the efficiency of vitellogenin synthesis by male quail
… Japanese quail were sacrificed and vitellogenin (phosphoprotein) production was assessed in these birds by analyzing plasma for protein-bound phosphorus concentrations (PBP). Plasma PBP concentrations of estrogen-injected male quails increased from undetectable values for control birds to …
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Development of Methods for the Study of Phosphoproteins
Characterization of phosphoproteins-including detection, identification of phosphoproteins and identification of phosphorylation sites-is mostly done with radiolabeling and proteomic techniques. Three main topics related to phosphoprotein characterization are included in this dissertation. First, …
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Insulin Receptor Substrate-1 Serine Phosphorylation by a Novel Phosphatidylinositol-3'-Kinase-Associated Serine Kinase Regulates Insulin and Interferon Receptor Signaling
… with a wortmannin insensitive 76 kDa serine phosphoprotein (pp76) distinct from the p85 subunit of PI 3-kinase. pp76 is phosphorylated by an okadaic acid sensitive, PI 3-kinase associated serine kinase (PAS kinase) with biochemical properties that distinguish it from the intrinsic serine …
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DEVELOPMENT AND OPTIMIZATION OF ON-PROBE AFFINTY CAPTURE (OPAC) MALDI MASS SPECTROMETRY FOR THE FRACTIONATION AND ANALYSIS OF COMPLEX PROTEIN MIXTURES
… sample. Taking a slightly different approach, a phosphoprotein binding OPAC probe was prepared using commercially available poly(methyl methacrylate) (PMMA) film. The hydrolyzed PPMA films were reacted with CuCl<sub>2</sub> solution to incorporate metal ions on the surface by electrostatic …
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