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Showing 1 to 20 of 33 for “"phosphopeptide"”.
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The Development of Functionalized Metal Complexes as Selective Phosphopeptide Molecular Recognition Agents
… metal complexes into functional and selective phosphopeptide recognition agents is described. Scaffold functionalization was conducted to assess whether binding affinity for phosphate monoesters could be modulated. A protocol for the facile synthesis of symmetric and asymmetric …
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White spot lesion regression using casein phosphopeptide amorphous calcium phosphate complexes alone or combined with microabrasion.
… (WSLs) can be improved by treatment with casein phosphopeptides (CPP) and amorphous calcium phosphate (ACP). The aim of this study was to test the null hypothesis that the treatment of WSLs with CPP-ACP complexes produces no difference in the outcome of WSL regression when compared to controls …
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Caged phosphopeptides and phosphoproteins : probes to dissect the role of phosphorylation in complex signaling pathways
… of a physiologically-relevant concentration of a phosphopeptide with temporal and spatial control is accomplished by the photolysis of a photolabile precursor, a caged phosphopeptide. The synthesis and application of NI-Fmoc-protected 1-(2-nitrophenyl) ethyl (NPE) caged phosphothreonine, serine, …
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Novel methods in computational analysis and design of protein-protein interactions : applications to phosphoregulated interactions
… application to the study of the interactions of phosphopeptides with phosphopeptide-binding domain interactions. A novel protein-protein interaction type, the action-at-a-distance interaction, is described in the complex of the TEM1 P-lactamase with the 3-lactamase inhibitor protein (BLIP). New …
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Assessing the Clinical Relevance of BRCA1 BRCT Domain Variants of Uncertain Significance
… VUSs to prioritize for functional analyses like phosphopeptide binding assays. All BRCA1 BRCT missense variants were collected from the ClinVar database and were analyzed using 50 different in silico tools. Molecular Feature Selection Tool (MFeaST) ranked tools based on their ability to …
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Analysis of The RING Domain And BRCT Repeats of BRCA1
… activity of BRCA1. The BRCT repeats have phosphopeptide binding activity, which is necessary for BRCA1's interaction with DNA repair proteins BACH1, ABRAXAS, and CtIP. To test the importance of these two domains we generated cell lines and mouse models with point mutations that either …
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Amphiphilic Cell-Penetrating Peptides Containing Natural and Unnatural Amino Acids as Drug Delivery Tools and Antimicrobial Agents
… the cellular uptake of fluorescence-labeled phosphopeptide (F´-GpYEEI), stavudine, lamivudine, emtricitabine, and siRNA in the presence of peptides with flow cytometry. The cellular uptake studies showed that [DipR]<sub>5</sub> significantly improved the uptake of F´-GpYEEI, by approximately …
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Hybrid Cyclic-Linear Cell-Penetrating Peptides Containing Alternative Positive and Hydrophobic Residues as Molecular Transporters
… <p>The cellular uptake of a fluorescence-labeled phosphopeptide (F′-GpYEEI) and anti-HIV drugs (lamivudine (F′-3TC), emtricitabine (F′-FTC), Stavudine (F′-d4T)), where F′ is carboxyfluorescein, were measured in the presence of the peptides in CCRF-CEM and SK-OV-3 cells. Among all peptides, …
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Effectiveness of Resin Infiltration and Mi Paste Cpp-Acp in Masking White Spot Lesions
… modalities, resin infiltration (RI) and Casein Phosphopeptide Amorphous Calcium Phosphate (CPP-ACP), at improving the light reflectivity and thus the appearance of WSLs using spectrophotometric analysis. Sixty extracted human third molars were partially demineralized to create artificial WSLs …
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The isolation and characterisation of MHC-presented peptides from CML-derived cell-lines, with a focus on post-translational modification
… myeloid leukaemia is a classic example. As phosphopeptides are known to be presented by both the MHC class-I and class- II pathways, against which specific CD4+ and CD8+ T cell responses may be generated, study of MHC-presented phosphopeptides may reveal unique cancer antigens with direct …
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Caged phosphopeptides and phosphoproteins : agents in unraveling complex biological pathways
… system. The syntheses and applications of caged phosphopeptides and phosphoproteins have been developed and detailed within this thesis. Two methods to synthesize 1-(2-nitrophenyl)ethyl caged phosphopeptides were developed. These peptides demonstrated good quantum yields of uncaging as compared …
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Regulation of Phosphorylation of PPARγ by CDK5 in Obesity
… non-obese), or from cell lines, as assessed by phosphopeptide analysis or immunoblot. I have tried multiple approaches to validate that CDK5 phosphorylates Ser273 of PPAR? but our data questions the clinical potential of targeting this modification.
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Two Redundant Ubiquitin-dependent Pathways of BRCA1 Localization to DNA Damage Sites
… manner, and coordinates DSB repair. The phosphopeptide-binding activity of the BRCA1 C-terminal (BRCT) domains is essential for ubiquitin-dependent recruitment and it is through these domains that BRCA1 indirectly interacts with RAP80, a ubiquitin-interacting protein. RAP80 targets BRCA1 …
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Metal-protein interactions for proteomics applications
… Affinity Capture with Ti(IV) (PolyMAC-Ti) for phosphopeptide enrichment in solution phase as opposed to commonly used solid phase extraction methods. In chapter 2 we discuss further extension of PolyMAC technology to PolyMAC-Zr and the use of complementary metal ion enrichment to study Syk …
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Spatial and temporal coordination of genome segregation with activation of the Mitotic Exit Network
… present evidence that Mob1 is a novel class of phosphopeptide binding domains. Thus, Cdc15-dependent phosphorylation of the scaffold Nud1 creates a docking site for the Mob1 coactivator, thereby resulting in the recruitment of Dbf2 to SPBs and firing of the MEN.
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Development of Non-Atp Competitive, Plk1-Selective Inhibitors
… mimetics for determinants from the endogenous phosphopeptide sequence, LLCSpTPNGL (CDC25c), known to specifically bind to the PBD of PLK1. Using this approach, FLIPs (Fragment Ligated Inhibitory Peptides) were generated and tested in a fluorescence polarization (FP) assay to determine binding …
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Computational methodologies and resources for discovery of phosphorylation regulation and function in cellular networks
… these clustering solutions and discovered that phosphopeptide co-clustering robustness can recapitulate known protein interaction networks, and extend them. Both of these tools take advantage of a new linear motif discovery algorithm, which we additionally used to find a putative regulatory …
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Global characterization of oncogenic kinase signaling
… Coupling the kinase enrichment with phosphopeptide enrichment enabled the identification of 799 kinase-derived phosphosites, with ~10% localized within the activation loop. Secondly, the global impact of constitutively active Src on the expressed kinome in MCF-10A cells was …
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Characterization of cellular stress systems using biological mass spectrometry
… not their exact identity. A combination of a phosphopeptide enrichment step and a MS-based approach was utilized to identify three phosphorylation sites on hYVH1, an atypical dual specificity phosphatase that functions as a cell survival factor. With the help of phosphomimetic and …
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Development of Tyrosine Kinase Peptide Biosensors and Methods for Detection
… and antenna for sensitization of Tb3+. The phosphopeptide displayed several phosphorylation-dependent photophysical changes resulting in enhanced Tb3+ luminescence. The use of Tb3+ and this sensor produced robust detection of Syk kinase activity and inhibition in vitro. An algorithm that …
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