Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"phospholipid membrane"”.
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Synthesis and Characterization of Miniaturized Fluorescence Sensors for Aqueous and Cellular Measurements
… dye, lucigenin, was immobilized into the phospholipid membrane of the lipobeads to enable chloride ion detection. The fluorescence intensity of lucigenin decreases with increasing chloride ion concentration due to dynamic quenching. To stabilize the lipobeads we co-immobilized …
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Miniaturized Fluorescence Biosensor for Studying Neuronal Events
… Zn2+ analysis. In the first approach, liposomes (phospholipid vesicles) were used as miniaturized containers for fluorescent sensing reagents. Liposome-based fluorescence sensing technology offers several advantages over commonly used fluorescence sensing techniques including high spatial …
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Blood coagulation reactions on nanoscale membrane surfaces
… coagulation requires the assembly of several membrane-bound protein complexes composed of regulatory and catalytic subunits. The biomembranes involved in these reactions not only provide a platform for these procoagulant proteins, but can also affect their function. Increased exposure of …
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Molecular dynamics studies of membranes and membrane proteins
Properties of phospholipid membrane bilayers and of proteins associating and functioning in the membrane bilayer or at the membrane-water interface are studied by combining molecular dynamics simulations, free energy perturbation theory and continuum electrostatics calculations. Three systems were …
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Synthetic Ion Channels From Lipophilic Guanosine Derivatives
… pores not only represent models of natural transmembrane ion channels, but also demonstrate their potential applications in the areas of drug delivery, biosensors, antimicrobial agents and other molecular devices. In this thesis, lipophilic guanosine derivatives that combine both "molecular …
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Identification and characterization of components of rab-6-mediated trafficking in caenorhabditus elegans
Membrane trafficking in neurons is an important mechanism used to regulate signaling. Controlling the abundance of AMPA-type receptors at the synapse is important for synaptic plasticity and influences processes involved in learning, memory formation, and motor control (Greger and Esteban, 2007; …
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Development of fluorescence lifetime imaging microscopy techniques for analysis of single extracellular vesicles
… (EVs) make up a variety of μm and sub-μm phospholipid membrane-bound particles released by cells. EVs have recently become an intense topic of interest as more is learned about their many purposes, such as their function in cell-to-cell communication in diseases such as cancer. EVs are …
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Fluorescence Resonance Energy Transfer (FRET) Based Sensors for Bioanalysis
… donor molecules truly permeate through the cell membrane or only adsorb to the cell surface. This method was found to be partially successful since the donor red tail fluorescence overlapped with the sensitized acceptor fluorescence and led to false reading of FRET. We found that is easier to …
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Structural and functional complexity in synthetic cells from amphiphilic DNA nanostars
… delimited from its surroundings *via* the phospholipid cell membrane. Sub-compartments within the cell, known as organelles, may also be membrane-bound or membrane-less. The combination of these structures makes a biological cell internally complex and enables intricate cellular …
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COMPUTATIONAL MODELING OF BACTERIAL OUTER MEMBRANES AND DEVELOPMENT OF HIGH-THROUGHPUT SCREENING PLATFORM FOR ANTIBIOTICS
… readily due to their unique highly charged outer membrane. Structurally, the Gram-negative bacteria is highly asymmetric bilayer that comprises of an inner leaflet of phospholipids and an outer leaflet of lipopolysaccharides. Embedded in the bilayer are outer membrane proteins (OMPs) that form …
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A Bottom-Up Approach to Building an Artificial Cell by Self-Assembly
… glycolipid has been incorporated into the phospholipid membrane of giant unilamellar vesicles at biologically relevant concentrations. The synthetic glycolipid shows concentration-dependent phase behaviour in binary mixtures with DOPC and in ternary mixtures with DOPC and cholesterol. At …
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Structural and kinetic studies of surfactant and phospholipid monolayers at the air/liquid interface using sum-frequency generation spectroscopy
… used to investigate a variety of surfactant and phospholipid systems at different interfaces. Firstly, the effects of temperature and ionic strength on the adsorption of cationic and non-ionic surfactants were investigated. The binary surfactant system used here is a simplified hair conditioner …
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Contribution of different types of phospholipids in blood coagulation
… interaction of blood clotting proteins with membranes of activated platelets is essential for maintaining a balanced hemostasis. Hemophilia and thrombosis are the two extremes of an imbalanced hemostasis where patients with hemophilia suffer from severe bleeding and those with thrombosis from …
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Water-Soluble Deep-Cavity Cavitands: Synthesis, Molecular Recognition, and Interactions with Phospholipid Membranes
… to learn how these molecules interact with phospholipid membranes. Six water-soluble cavitands were tested for their ability to permeabilize liposomal POPC membranes. One host showed very high potency in permeabilizing membranes, while three other hosts showed moderate activity. Host binding …
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Design and Synthesis of a Macrocyclic Phospholipid
The membrane-spanning phospholipids of the domain Archaea are postulated to provide membrane stability; this thesis reports the design and synthesis of a synthetic membrane-spanning macrocyclic lipid to test this hypothesis. Protected glutaric anhydride reacted with 10-undecyn-1-ol to produce a …