Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 108 for “"phosphoinositide"”.
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Identification and characterization of phosphoinositide-binding Legionella effector proteins
… is their ability to recognize and bind host phosphoinositides (PIPs) (Steiner, Weber, & Hilbi, 2018), which are a series of phospholipids that play critical roles in coordinating cell signaling and membrane trafficking events in eukaryotic cells (Di Paolo & De Camilli, 2006). L. pneumophila …
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Investigating the isoform-specific role of phosphoinositide 3- kinase p110delta
Phosphoinositide 3-kinases (PI3Ks) are a family of enzymes that regulate a diverse array of biological functions in every cell type by generating lipid second messengers. The PI3K family is comprised of multiple isoforms that are divided into three main classes; class I, II and III. All class I …
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Regulation of LFA-1 on T cells by phosphoinositide signalling
… of T cells. LFA-1 activation is mediated by phosphoinositide 3-kinase (PI3K) and the downstream phosphoinositide PtdIns(3,4,5)P3 (PIP3). To investigate how PI3K regulates LFA-1, I optimised CRISPR/Cas9-mediated mutagenesis in T cells, and designed a retroviral library of CRISPR/single guide …
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Carbachol- and ACPD-Induced Phosphoinositide Responses in the Developing Rat Neocortex
Signal transduction via the phosphoinositide (PI) second messenger system has key roles in the development and plasticity of the neocortex. The present study localized PI responses to individual cortical layers in slices of developing rat somatosensory cortex. The acetylcholine agonist carbachol …
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Localisation and function of phosphoinositide-specific phospholipase C in Sacchromyces cerevisiae
Phosphoinositide-specific phospholipase C enzymes (PLCs) cleave the plasma membrane phospholipid PtdIns(4,5)P\(_2\) to generate two messengers, inositol (1,4,5) trisphosphate [Ins(1,4,5)P\(_3\)] and diacylglycerol (DAG). Ins(1,4,5)P\(_3\) is an important second messenger in animal cells. It …
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A screen for phosphatidylinositol 3,4-bisphosphate and other 3-phosphoinositide effector proteins
Class I phosphoinositide (PI) 3-kinases exert profound effects on cell growth, division, motility and metabolism via their primary lipid product phosphatidylinositol 3,4,5- trisphosphate (PtdIns(3,4,5)P3) and a metabolite of this, phosphatidylinositol 3,4- bisphosphate (PtdIns(3,4)P2). Many …
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Redefining the specificity of phosphoinositide-binding by human PH domain-containing proteins
… (PH) domain, a lipid-binding domain, with phosphoinositides (phosphatidylinositol phosphates or PIPs), an important class of regulatory phospholipids. PH domains are presumed to bind PIPs, but specific interaction with and regulation by PIPs for most PH domain-containing protein remains …
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Trypanosoma Cruzi Phosphoinositide -Specific Phospholipase C (tcpi-Plc) and Its Role in Differentiation
A newly discovered Trypanosoma cruzi phosphoinositide phospholipase C (TcPI-PLC) is lipid modified in its N-terminus, and located in the plasma membrane of amastigotes. The N-terminal 20 amino acids of TcPI-PLC contain primary structural information sufficient to target the heterologous Aequorea …
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An Investigation into the Function of CDS2 in Phosphoinositide Synthesis in Murine Macrophages
Phosphoinositides (PIPn) are a branch of membrane phospholipids involved in regulating critical cellular functions, such as Phosphoinositide 3-kinase (PI3K) and Phospholipase C (PLC) signalling pathways, the definition of sub-cellular membrane identity and the sorting of proteins and lipids within …
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Interactome Analysis Identifies Novel Targets of Phosphoinositide 3-kinase (PI3K) that Mediate Astrocyte Neuroprotection
… Here, a chemical genetics screen revealed phosphoinositide 3-kinase (PI3K) as a central player transducing ACM-mediated neuroprotection. To identify additional proteins contributing to the protective activity, endogenous neuronal PI3K was immunoprecipitated from astrocytes exposed to ACM or …
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A Role for the Phosphoinositide Lipid Kinase PI4KIIIbeta in Breast Oncogenesis and Akt Activation
The lipid kinase phosphatidylinositol 4-kinase III β (PI4KIIIβ) phosphorylates phosphatidylinositol (PtdIns) to generate PI(4)P in the Golgi. PI4KIIIβ is likely involved in the development of breast cancer as it has been reported genetically amplified in a subset of human breast tumours and is a …
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A Phosphoinositide-Specific Phospholipase C of Trypanosoma Cruzi: Unique Characteristics of a Delta-Type Homologue
The phosphoinositide-specific phospholipase C gene (TcPI-PLC  ) of the protozoan parasite Trypanosoma cruzi was cloned, sequenced, expressed in E. coli, and the protein product (TcPI-PLC) was shown to have enzymatic characteristics similar to those of mammalian delta-type PI-PLCs. The …
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The Effect of Manipulation of the Phosphoinositide 3-kinase Pathway on Axon Regeneration in vivo
… has demonstrated that manipulation of the phosphoinositide 3-kinase (PI3K) pathway by transgenic knockout of phosphatase and tensin homolog (PTEN) promotes survival and regeneration of murine RGC axons in an optic nerve crush (ONC) model. However, translation issues are yet to be addressed. …
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A dissection of class I phosphoinositide 3-kinase signalling in mouse embryonic fibroblasts and prostate organoids
Class I PI3Ks are a family (α, β, δ and γ) of ubiquitous lipid kinases that can be activated by cell surface receptors to 3-phosphorylate PI(4,5)P2 (phosphatidylinositol(4,5)-bisphosphate) and generate the signalling lipid PI(3,4,5)P3. The PI(3,4,5)P3 signal then activates a diverse collection of …
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The Role of ER-Golgi Membrane Contact Sites and of FAPP1 in Phosphoinositide Homeostasis at the Golgi Complex
Initially considered to be a precursor for highly phosphorylated phosphatidylinositol species, phosphatidylinositol 4-phosphate (PI4P) turned out to be “active” in its own right as a pivotal regulator of multiple cellular functions including membrane trafficking, sphingolipid metabolism, autophagy, …
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The role of PTEN as a PI(3,4)P2 lipid phosphatase in Class I phosphoinositide 3-kinase signalling
… PTEN as a PI(3,4)P2 lipid phosphatase in Class I phosphoinositide 3-kinase signalling Abstract Class I phosphoinositide 3-kinases (Class I PI3Ks) are essential players involved in the signalling events in the cell and are critical promoters of cellular growth, survival and metabolism. Once …
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Structural basis of membrane targeting and regulation of the innate immunity adaptor TIRAP by its phosphoinositide-binding motif
… and other adaptor proteins; and a N-terminal phosphoinositide-binding motif (PBM) that mediates the membrane recruitment of TIRAP. Upon ligand activation, TLRs are recruited to the phosphoinositide (PIP)-enriched region in the membrane, where TIRAP recruits other adaptors to the membrane to …
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