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Showing 1 to 12 of 12 for “"phosphoenolpyruvate carboxylase"”.
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Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System
… acid (TCA) cycle, specifically the enzymes phosphoenolpyruvate carboxykinase, pyruvate carboxylase and phosphoenolpyruvate carboxylase. All produce oxaloacetate (OAA) for the cell. OAA production is critical for cell carbon synthesis in the methanogenic archaea. Therefore OAA-generating …
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Investigation of the mechanism of action of pep carboxylase
Phosphoenolpyruvate carboxylase (orthophosphate: oxaloacetic acid carboxylyase, E.C.4.1.2.31) was isolated from 4-day germinated peanut cotyledons and purified about 2400-fold. A large-scale purification procedure has been developed in order to obtain large quantities of the highly-purified enzyme. …
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Application of metabolic and biochemical engineering techniques to enhance metabolic flux throughput and improve succinate production in Escherichia coli
… to the system and overexpression of the phosphoenolpyruvate carboxylase (PEPC) enzyme system coupled with pathway manipulations and use of different carbon sources. Incorporation of the glcU gene from Staphylococcus xylosus encoding a novel glucose uptake protein resulted in enhancement …
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Structure-Function Studies on Two Phosphoenolpyruvate Carboxylases
Phosphoenolpyruvate carboxykinase (PEPCK) and phosphoenolpyruvate carboxylase (Pepc) are two important CO₂-fixation enzymes which share a similar reaction mechanism. Both operate through a lid-gated active site and have a hypothesized enol-pyruvate intermediate in their catalytic pathway. While …
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Metabolic network design and engineering in Escherichia coli
… PEP in order to improve succinate synthesis. Phosphoenolpyruvate carboxylase and pyruvate carboxylase were coexpressed to drive the carbon flux toward succinate. Competing pathways of succinate synthesis, the lactate and acetate pathways, were also inactivated to increase more carbon flux …
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Developmental and Expression Evolution in C3 and C4 Atriplex and their Hybrids
… and bundle sheath. Mesophyll cells contain phosphoenolpyruvate carboxylase, the primary carbon fixation enzyme, whereas bundle sheath contains Rubisco and houses photosynthetic carbon reduction. I used C3 x C4 Atriplex F1 hybrids and allele-specific expression analysis to investigate the …
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Characterizing the transcriptional regulation of crassulacean acid metabolism in Kalanchoe
… governing the transcriptional control of the phosphoenolpyruvate carboxylase gene (Ppc1) in Kalanchoe. Despite this enzyme's importance in catalyzing the primary nocturnal fixation of CO2 in CAM species, the complex regulatory mechanisms underlying its expression are not well-studied. We …
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Characterisation of protein phosphorylation in Arabidopsis thaliana and Chlamydomonas reinhardtii based on affinity chromatography and mass spectrometry
… phosphorylation site of the metabolic key enzyme phosphoenolpyruvate carboxylase in A. thaliana could be confirmed. While the detection of phosphorylation and the determination of phosphorylation sites are very important to get a first impression whether or not a protein can be influenced by …
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Metabolic flux analysis of Escherichia coli MG1655 under octanoic acid (C8) stress
… by ~ 44%; the malic enzyme pathway by ~ 80%; the phosphoenolpyruvate carboxylase pathway ~ 60%; the CO2 production rate by 18%; and pyruvate dehydrogenase pathway by ~50%. Meanwhile, a few pathways were activated under C8 stress: the pyruvate dehydrogenase flux (`PoxB') became active; the malate …
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PHYSIOLOGICAL STUDIES USING TISSUE CULTURES OF PLATYCERIUM CORONARIUM (KOENIG) DESV
… of chloroplasts and ribulose bisphosphate carboxylase (RuBPC) activity. Under prolonged CO2 enriched conditions, photoautotrophic callus of P. coronarium showed an increase in biomass and phosphoenolpyruvate carboxylase (PEPC) activity but a decrease in total chlorophyll content, …
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Insights into substrate utilization and transcriptional regulation in methanosarcina
… of pyruvate to acetyl-CoA and lacked pyruvate carboxylase activity, the enzyme responsible for synthesis of oxaloacetate in the cells. Biochemical and transcriptomic experiments revealed that in order to make the central metabolite oxaloacetate this mutant was using an alternative reaction …