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Showing 1 to 19 of 19 for “"phosphoenolpyruvate carboxylase"”.
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Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System
… acid (TCA) cycle, specifically the enzymes phosphoenolpyruvate carboxykinase, pyruvate carboxylase and phosphoenolpyruvate carboxylase. All produce oxaloacetate (OAA) for the cell. OAA production is critical for cell carbon synthesis in the methanogenic archaea. Therefore OAA-generating …
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Investigation of the mechanism of action of pep carboxylase
Phosphoenolpyruvate carboxylase (orthophosphate: oxaloacetic acid carboxylyase, E.C.4.1.2.31) was isolated from 4-day germinated peanut cotyledons and purified about 2400-fold. A large-scale purification procedure has been developed in order to obtain large quantities of the highly-purified enzyme. …
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Phosphoenolpyruvat-Carboxylasen in Solanum tuberosum : Charakterisierung und Expression
… carboxylation reaction catalysed by the enzyme phosphoenolpyruvate-carboxylase (PEPC). This thesis presents research into increasing this enzyme’s activity in S.tuberosum. Two clones carrying the stppc1 or the stppc2 gene were isolated from a S.tuberosum genomic phage bank by plaque and Southern …
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Die epigenetische Regulation des C 4 -Syndroms : belichtungsabhängige Chromatinveränderungen am Promotor der Phosphoenolpyruvat Carboxylase aus Mais (Zea mays L.)
… in the mesophyll cytoplasm and is controlled by phosphoenolpyruvate carboxylase (PEPC). The enzyme catalyses the formation of oxaloacetate from bicarbonate and phosphoenolpyruvate (PEP). In maize, the reaction product is rapidly converted to malate and transported into the bundle sheath where it …
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Expression rekombinanter pflanzlicher Phosphoenolpyruvat-Carboxylasen zur Beeinflussung des Kohlenstoffmetabolismus von Solanum tuberosum
Phosphoenolpyruvate carboxylase (PEPC) is a ubiquitous cytosolic enzyme in plants. Besides several housekeeping functions in plant metabolism, PEPC plays a cardinal role in the initial fixation of CO2 in C4 and CAM plants. The goal of this work was to regenerate transgenic C3-plants (Solanum …
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Application of metabolic and biochemical engineering techniques to enhance metabolic flux throughput and improve succinate production in Escherichia coli
… to the system and overexpression of the phosphoenolpyruvate carboxylase (PEPC) enzyme system coupled with pathway manipulations and use of different carbon sources. Incorporation of the glcU gene from Staphylococcus xylosus encoding a novel glucose uptake protein resulted in enhancement …
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Structure-Function Studies on Two Phosphoenolpyruvate Carboxylases
Phosphoenolpyruvate carboxykinase (PEPCK) and phosphoenolpyruvate carboxylase (Pepc) are two important CO₂-fixation enzymes which share a similar reaction mechanism. Both operate through a lid-gated active site and have a hypothesized enol-pyruvate intermediate in their catalytic pathway. While …
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Metabolic network design and engineering in Escherichia coli
… PEP in order to improve succinate synthesis. Phosphoenolpyruvate carboxylase and pyruvate carboxylase were coexpressed to drive the carbon flux toward succinate. Competing pathways of succinate synthesis, the lactate and acetate pathways, were also inactivated to increase more carbon flux …
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Studies to influence the carbon metabolism of the C 3 plant Nicotiana tabacum by expressing heterologous enzymes involved in C 4 -like CO 2 fixation
… pathway ("C4-cycle"). These enzymes include phosphoenolpyruvate carboxylase (PC), NADP-malic enzyme (ME), pyruvate, orthophosphate dikinase (DK), phosphoenolpyruvate synthase (PS), phosphoenolpyruvate carboxykinase (CK) and phosphoenolpyruvate translocator (PT). By individual gene …
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Developmental and Expression Evolution in C3 and C4 Atriplex and their Hybrids
… and bundle sheath. Mesophyll cells contain phosphoenolpyruvate carboxylase, the primary carbon fixation enzyme, whereas bundle sheath contains Rubisco and houses photosynthetic carbon reduction. I used C3 x C4 Atriplex F1 hybrids and allele-specific expression analysis to investigate the …
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Characterizing the transcriptional regulation of crassulacean acid metabolism in Kalanchoe
… governing the transcriptional control of the phosphoenolpyruvate carboxylase gene (Ppc1) in Kalanchoe. Despite this enzyme's importance in catalyzing the primary nocturnal fixation of CO2 in CAM species, the complex regulatory mechanisms underlying its expression are not well-studied. We …
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Metabolic engineering aimed at the production of keto acids from glycerol : an industrial by-product
… activities of various enzymes including pyruvate carboxylase (PC) and phosphoenolpyruvate carboxylase (PEPC) that were analyzed by blue-native polyacrylamide gel electrophoresis (BN-PAGE) and high performance liquid chromatography (HPLC). The high-energy phosphoenolpyruvate (PEP) is then converted …
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Beitrag der Histonacetylierung zur Signalintegration auf dem C 4-spezifischen Phosphoenolpyruvat-carboxylase Gen in Mais (Zea mays L.)
The expression of the C4-specific phosphoenolpyruvate carboxylase gene (C4-Pepc) in maize (Zea mays L.) is regulated by multiple internal and external stimuli. C4-Pepc gene expression is restricted to mesophyll cells within foliar leaves but its activity is further induced by light and modulated by …
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Characterisation of protein phosphorylation in Arabidopsis thaliana and Chlamydomonas reinhardtii based on affinity chromatography and mass spectrometry
… phosphorylation site of the metabolic key enzyme phosphoenolpyruvate carboxylase in A. thaliana could be confirmed. While the detection of phosphorylation and the determination of phosphorylation sites are very important to get a first impression whether or not a protein can be influenced by …
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Die C 4 -spezifische Phosphoenolpyruvatcarboxylase in Zea mays L.: Modellsystem zur Signaintegration auf Chromatinebene in Pflanzen
… activity of the C4-specific isoform of phosphoenolpyruvate carboxylase (C4-Pepc) and have to be integrated into a single promoter response. Therefore, C4-Pepc is a perfect candidate to investigate signal integration on the chromatin level. Previous studies in our group already revealed a …
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Metabolic flux analysis of Escherichia coli MG1655 under octanoic acid (C8) stress
… by ~ 44%; the malic enzyme pathway by ~ 80%; the phosphoenolpyruvate carboxylase pathway ~ 60%; the CO2 production rate by 18%; and pyruvate dehydrogenase pathway by ~50%. Meanwhile, a few pathways were activated under C8 stress: the pyruvate dehydrogenase flux (`PoxB') became active; the malate …
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PHYSIOLOGICAL STUDIES USING TISSUE CULTURES OF PLATYCERIUM CORONARIUM (KOENIG) DESV
… of chloroplasts and ribulose bisphosphate carboxylase (RuBPC) activity. Under prolonged CO2 enriched conditions, photoautotrophic callus of P. coronarium showed an increase in biomass and phosphoenolpyruvate carboxylase (PEPC) activity but a decrease in total chlorophyll content, …
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Insights into substrate utilization and transcriptional regulation in methanosarcina
… of pyruvate to acetyl-CoA and lacked pyruvate carboxylase activity, the enzyme responsible for synthesis of oxaloacetate in the cells. Biochemical and transcriptomic experiments revealed that in order to make the central metabolite oxaloacetate this mutant was using an alternative reaction …