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Showing 1 to 20 of 53 for “"phosphoenolpyruvate"”.
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Structural and functional studies of phosphoenolpyruvate carboxykinase
ATP-dependent phosphoenolpyruvate carboxykinase (E. C. 4.1.1.49; PCK) is an enzyme that catalyses the reversible conversion of oxaloacetate and ATP into phosphoenolpyruvate, ADP and CO2. PCK is made up of about 500 to 600 amino acid residues and is divided into two roughly equal domains. Upon …
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The purification and characterization of phosphoenolpyruvate carboxykinase
Phosphoenolpyruvate carboxykinase has been isolated from pig liver mitochondria and purified 208-fold from the initial mitochondrial accectone powder extract in good yield (48%). The enzyme appears homogeneous in sedimentation velocity experiments and has a sedimentation coefficient (s₂₀⁰,w) of …
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Structure-Function Studies on Two Phosphoenolpyruvate Carboxylases
Phosphoenolpyruvate carboxykinase (PEPCK) and phosphoenolpyruvate carboxylase (Pepc) are two important CO₂-fixation enzymes which share a similar reaction mechanism. Both operate through a lid-gated active site and have a hypothesized enol-pyruvate intermediate in their catalytic pathway. While …
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Proteins and instability elements involved in phosphoenolpyruvate carboxykinase mRNA turnover
Phosphoerco/pyruvate carboxykinase catalyzes a rate-limiting step in hepatic and renal gluconeogenesis. However, this activity is not regulated by allosteric mechanisms or by covalent modifications. Instead, it is regulated by mechanisms that affect the level of the PEPCK m RNA and subsequently …
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Non-Canonical Roles of Cytosolic Phosphoenolpyruvate Carboxykinase in Small Intestine Metabolism
Phosphoenolpyruvate carboxykinase (PEPCK) is considered by many to be the quintessential regulatory enzyme of gluconeogenesis. This common perspective of PEPCK solely functioning in gluconeogenesis is too narrow and does not do justice to the complexity and number of pathways in which this enzyme …
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Propionate induces gluconeogenesis in dairy cattle through direct activation of the bovine cytosolic phosphoenolpyruvate carboxykinase gene promoter
<p>Phosphoenolpyruvate carboxykinase (PCK, GTP; EC4.1.1.32) catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), a critical reaction for gluconeogenesis. The two isoforms of PCK, namely cytosolic PCK (PCK1), and the mitochondrial PCK (PCK2) are abundantly expressed in liver …
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Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System
… acid (TCA) cycle, specifically the enzymes phosphoenolpyruvate carboxykinase, pyruvate carboxylase and phosphoenolpyruvate carboxylase. All produce oxaloacetate (OAA) for the cell. OAA production is critical for cell carbon synthesis in the methanogenic archaea. Therefore OAA-generating …
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Investigation of the mechanism of action of pep carboxylase
Phosphoenolpyruvate carboxylase (orthophosphate: oxaloacetic acid carboxylyase, E.C.4.1.2.31) was isolated from 4-day germinated peanut cotyledons and purified about 2400-fold. A large-scale purification procedure has been developed in order to obtain large quantities of the highly-purified enzyme. …
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Investigations into the biosynthesis and mode of action of the phosphonate antibiotic dehydrophos
… library of S. luridus for the presence of the phosphoenolpyruvate mutase gene, which is required for biosynthesis of most phosphonates. Integration of one such fosmid clone into the chromosome of Streptomyces lividans led to heterologous production of dehydrophos. Deletion analysis of this …
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Structure and function of enzyme I of the PTS
The phosphoenolpyruvate: sugar phosphotransferase system (PTS) is responsible for the uptake and concomitant phosphorylation of many sugars in several species of bacteria. The first step of the PTS involves the phosphorylation of histidine containing phosphocarrier protein (HPr) by enzyme I (E.C. …
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Chemotactic Sensory Transduction in Bacillus Subtilis: A New Enzyme and a New Pathway
Chemotaxis towards substrates of the phosphoenolpyruvate-dependent-phosphotransferase system (PTS) in E. coli is accomplished using a chemoreceptor-independent signaling circuit in which phosphoryl flux through the PTS is monitored and used directly to modulate the activity of the chemotactic …
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The effect of magnesium deficiency on the gluconeogenic enzymes with emphasis on the effects of fasting and anorexia
… fructose 1, 6-bisphosphatase (FDPase), and phosphoenolpyruvate carboxykinase (PEPCK). In Experiments II and III rats were meal-fed diets adequate or deficient in magnesium; in addition, a group of rats was pair-fed to the magnesium-deficient group to test for the effects of anorexia. After …
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Application of metabolic and biochemical engineering techniques to enhance metabolic flux throughput and improve succinate production in Escherichia coli
… to the system and overexpression of the phosphoenolpyruvate carboxylase (PEPC) enzyme system coupled with pathway manipulations and use of different carbon sources. Incorporation of the glcU gene from Staphylococcus xylosus encoding a novel glucose uptake protein resulted in enhancement …
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The effects of sodium cations on rabbit muscle enolase : evidence for the binding of 2PGA to apo-enolase with Na⁺
… of 2-phospho-D-glycerate (2PGA) to form phosphoenolpyruvate (PEP) in the glycolytic pathway. Studies in the inactivation of rabbit muscle enolase by NaClO$\sb4$ show that both Mg$\sp{2+}$ and 2PGA protect the enzyme from the inactivation. The protection provided by Mg$\sp{2+}$ and 2PGA is …
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Biochemistry and regulation of CheA in Bacillus subtilis chemotaxis
… sugars is mediated solely through a separate phosphoenolpyruvate-dependent phosphotransferase system (PTS), involved in sugar transport. In B. subtilis, we have determined that taxis toward PTS carbohydrates is mediated both by MCPs and the PTS. Finally, a restriction map of the distal end of …
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Characterization of botryococcus sp. and identification of carbohydrates metabolic related enzymes toward lipid production
… A glycolytic enzyme ‘Enolase ’which can generate phosphoenolpyruvate (PEP) and then convert it into pyruvate was identified in this study. Enolase which helps in high lipid metabolism was found in the cytoplasm and was used to construct the alternative pathway. Enolase was found to export fixed …
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Chemical and biochemical applications of vibrational spectroscopy
… analysis was first performed on the substrate, phosphoenolpyruvate (PEP) in a buffer solution, using data from Fourier-transform infrared (FTIR) spectroscopy with an attenuated total reflectance (ATR) accessory. This method was then used to monitor PEP binding to rabbit muscle pyruvate kinase …
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Carbon dioxide fixation and glycogenesis in the flatworm Hymenolepis diminuta
… and the dicarboxylic acid shuttle. An active phosphoenolpyruvate carboxykinase, similar to the one present in avian liver, was found to be present in H. diminuta. This activity was stimulated by starvation and was abundant enough to account for the in vivo incubation results. A low level of …
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