Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"phosphinothricin"”.
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Genetic and Biochemical Analysis of Phosphinothricin Tripeptide Biosynthesis in Streptomyces Viridochromogenes
Our investigations led to the discovery of new biosynthetic intermediates and revealed inaccuracies in the previously published pathway. Specifically, this thesis work established that step III of PTT biosynthesis is the reduction of phosphonoacetaldehyde to hydroxyethylphosphonate by PhpC, an …
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Investigation of phosphonate biosynthesis: I. Structure of dehydrophos II. Mechanism of hydroxyethylphosphonate dioxygenase
… cleavage reaction as part of the early steps of phosphinothricin biosynthesis. Characterization of HEPD has shown it to be a non-heme iron dependent dioxygenase that is dependent on only ferrous iron and molecular oxygen for activity. Studies with substrate isotopologues and substrate analogues …
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Mechanistic investigations of enzymes in phosphonate metabolism
… HEP) during the biosynthesis of the herbicide phosphinothricin. Mechanistic studies were undertaken to elucidate the mechanism of catalysis by HEPD. These studies demonstrated that an unusual iron(IV)-oxo intermediate is at the heart of the catalytic cycle of HEPD. Also reported in this …
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Biochemische und zellbiologische Charakterisierung des COP Vesikel vermittelten Proteintransports in Pflanzen
… Proteins alpha-Amylase, des "bulk-flow" Markers Phosphinothricin-Acetyltransferase (PAT) und des ER residenten Proteins Calreticulin konnte weiterhin gezeigt werden, daß der ER Export nicht auf einem selektiven Mechanismus basiert, sondern durch "bulk-flow" erfolgt.Diese Ergebnisse lassen darauf …
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Effect of Temperature and Chemical Additives on the Efficacy of the Herbicides Glufosinate and Glyphosate in Weed Management of Liberty-Link and Roundup-Ready Soybeans
… because of the incorporation of a gene encoding phosphinothricin acetyl-transferase (pat), which is able to detoxify glufosinate. Roundup-Ready ® soybeans are transformed with an altered, non-sensitive form of 5-enolpyruvylshikimate- 3-phosphate synthase (EPSPS), which confers glyphosate …
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Investigations into the use of maize streak virus as a gene vector
… The cellular levels of the bar gene product, phosphinothricin acetyl transferase (PAT), were significantly enhanced in lines where the gene was amplified by linkage to an MSV replicon in comparison with lines where the same gene was not amplified. Northern blot analysis also showed that higher …
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Studies on improvement of Chinese wheat cultivars for resistance against an endemic virus using transgenesis and virus-wheat interactions at the molecular and ultrastructural levels
… selectable marker. Plants were regenerated under phosphinothricin (PPT) selection. The transgenic nature of the regenerated wheat plants was demonstrated by Southern hybridization analysis. Expression of viral CP gene was confirmed from most CP-transgenic wheat by RT-PCR testing, while only one …
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Investigation of the biosynthesis of the phosphonate antibiotics bialaphos and dehydrophos
… such compounds, PTT, contains unusual amino acid phosphinothricin attached to two alanine residues. The peptide bond formation and methylation on phosphorus in PTT are key steps in PTT biosynthesis, however, the exact timing of these transformations was not known. To provide insights into these …
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Transformation einer argentinischen Sommerweizenvarietät (Triticum aestivum L.) durch Transfer eines Seneszenz-verzögernden Gens zur Verbesserung der Ertragsleistung
… und ex vitro Kultur. Die Verwendung von Phosphinothricin (PPT) als Selektionsmittel während der ex vitro Kultur minimierte die Anzahl falscher Positiver unter den überlebenden Pflanzen (Cotransformanten 97,4%). Allerdings ließen sich durch unabhängige Segregation keine Reporter- bzw. …