Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 84 for “"phosphatidylserine"”.
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Characterization of Phosphatidylserine Expression in Bovine Sperm
… of this work was to explore the expression of phosphatidylserine (PS) on bovine sperm and investigate what factors may impact it, as previous research demonstrated that phosphatidylserine (PS) plays a role in murine fertilization. Despite conventionally being an apoptotic marker, it is present …
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Targeting phosphatidylserine exposure in pro-coagulant platelets.
… They expose the anionic phospholipid, phosphatidylserine (PS), in their outer leaflet enabling coagulation complex assembly on the negatively charged surface. Assembly of the tenase and prothrombinase complexes enhances their activity, increasing thrombin generation. PS is normally …
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Understanding the mechanisms of phosphatidylserine exposure in sickle cells
… blood cells’ (RBCs) membrane permeability, high phosphatidylserine (PS) exposure, altered RBC rheology and fragility. The high PS exposure is considered to cause some of the hallmark complications of the disease such as vascular occlusion, anaemia and inflammation. This study investigates the …
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Targeting Plasma Membrane Phosphatidylserine Content to Inhibit Oncogenic Kras Function
… lipid-binding specificity for select species of phosphatidylserine (PtdSer). We therefore investigated whether reducing PM PtdSer content is sufficient to abrogate KRAS oncogenesis. Oxysterol-related binding proteins ORP5 and ORP8 exchange PtdSer synthesized in the ER for …
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Structural effects of neutral lipids on the divalent cation-induced interactions of phosphatidylserine - containing bilayers
As Ca2+ and phosphatidylserine (PS) are known to induce the adhesion of bilayer vesicles and form collapsed multibilayer structures in vitro, it was the aim of this study to examine how that interaction and the resultant structures might be modified by neutral lipid species. X-ray diffraction data …
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Formation of membrane domains during the activation of protein kinase C
… (the MARCKS peptide) and its three activators (phosphatidylserine, Ca$\sp{2+}$ and DAG) in large unilamellar vesicles were investigated. The formation of phosphatidylserine domains could be induced by either Ca$\sp{2+},$ the MARCKS peptide, or protein kinase C. However, only Ca$\sp{2+}$ could …
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Investigating the effects of TREM2, APOE and PILRα variants on microglial functions and neuronal loss
… of isolated synapses (synaptosomes) with exposed phosphatidylserine, and increased uptake of phosphatidylserine-beads. Phosphatidylserine is a ligand of TREM2 and an "eat-me" signal exposed on the surface of apoptotic cells and stressed-but- viable neurons. Addition of BV-2 microglia, expressing …
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Mechanisms by Which Apoptotic Membranes Become Susceptible to Secretory Phospholipase A2
… increased merocyanine 540 intercalation; phosphatidylserine flip-flop, detected by annexin binding; and alterations in laurdan fluorescence properties. These observations implied a relationship among sPLA2 susceptibility, lipid spacing, and phosphatidylserine exposure. To clarify this …
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Loss of Ptdss1 in tumor cells improves anti-PD-1 therapy
<p>PTDSS1 (Phosphatidylserine synthase 1) encodes an enzyme that facilitates production of phosphatidylserine (PS), which mediates a global immunosuppressive signal. Here, based on in vivo CRISPR screen, we identified PTDSS1 as a target to improve anti-PD-1 therapy. Depletion of PTDSS1 in tumor …
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Role of CD47 In Red Cell Apoptosis (Eryptosis)
… new CD47 binding proteins that may mediate the phosphatidylserine exposure pathway (eryptosis is the process of red cell death which involves the switch of phosphatidylserine from the inner leaf of the membrane to the outer leaf), investigate the functions of the red cell CD47 complexes …
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Apoptotic cells promote circulating tumor cell survival and metastasis
… Apoptotic cells externalize the phospholipid phosphatidylserine to the outer leaflet of the plasma membrane, which we found increased the activity of the coagulation initiator Tissue factor, thereby triggering the formation of platelet clots that protect proximal CTCs. Inhibiting the ability …
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Discovery of novel antibacterial and antitubercular compounds using chemical genetics and computational approach targeted at phospholipid biosynthesis
… Genetic and biochemical studies carried out on phosphatidylserine synthase (Pss) and phosphatidylserine decarboxylase (Psd) demonstrated the essentiality of these membrane proteins in S. coelicolor. Alteration of pss expression affects the overall growth and morphology of S. coelicolor (i.e. …
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The relationship between docohexanoic acid (DHA) and L-serine, providing an insight into the biochemistry of meningioma
… tissue revealed unusually high levels of the phosphatidylserine enriched with docosahexaenoic acid (DHA). In this study, the metabolism surrounding lipid biosynthesis was examined to establish if such alterations in lipid profiles were related to an altered state of metabolism. From the …
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NMR investigations of blood coagulation: Conformational changes of membrane bilayers and proteins in blood coagulation
… and dynamics data of Ca2+-induced clusters of phosphatidylserine in membranes, important in blood clotting, is investigated using isotopically 13C, 15N-labeled phosphatidylserine in lipid bilayers. Finally, backbone resonances of the soluble, extracellular domain of tissue factor (absent the …
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Increased Apoptosis in the Host By Guanidine Hydrochloride Treatment of Infectious Pancreatic Necrosis Virus
… type of cell death, characterized early by phosphatidylserine flipping to the outer leaflet of the membrane, followed by cell shrinkage and cell blebbing. DNA isolates from apoptotic cells typically present a laddering effect on agarose gels. Recent publications indicate the early protein …
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Effect of phospholipase C papain on feathering defect in 11% homogenized UHT processed cream
… the proportions of phosphatidylinositol and phosphatidylserine in feathering cream were slightly less than in stable cream. Feathering cream had more caseins associated with milk fat globule than stable cream and more protein bands were observed in feathering cream than in stable cream. …
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Arenavirus Attenuation Mechanisms
… are covered with an indicator of apoptosis, phosphatidylserine. We further demonstrate that phosphatidylserine targeting antibodies are ineffective at protecting a small animal model against the disease or death due to virulent Junín virus infection.
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Apoptosis Pathways: Presence and significance in Ejaculated Human Spermatozoa
… caspase activity, flow cytometric evaluation of phosphatidylserine translocation, immunoblots, and immunocytochemistry were used in this investigation.</p> <p>Of the pro-apoptosic treatments, only staurosporine resulted in elevated caspase activity in patients and donors. Differences in caspase …
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