Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 14 of 14 for “"phosphatidylinositol 3-phosphate"”.
-
Phosphatidylinositol 3-phosphate binding properties and autoinhibition mechanism of Phafin2
… Both the Phafin2 PH and FYVE domains bind phosphatidylinositol 3-phosphate [PtdIns(3)P], a phosphoinositide mainly found in endosomal and lysosomal membranes. Phafin2 acts as a PtdIns(3)P effector for endosomal cargo trafficking, macropinocytosis, apoptosis, and autophagy. The PtdIns(3)P …
-
Structural basis for interactions of the Phytophthora sojae RxLR effector Avh5 with phosphatidylinositol 3-phosphate and for host cell entry
… resident in the host plasma membrane such as phosphatidylinositol 3-phosphate (PtdIns(3)P). Recent reports differ regarding the regions on RxLR effector proteins involved in PIP recognition. To clarify these differences, I have structurally and functionally characterized the P. sojae effector, …
-
Illuminating Organellar and Molecular Organization of the Endomembrane System
… for different membrane compartments. I show that phosphatidylinositol 3-phosphate is presnt on late endosomes while phosphatidylinositol 4-phosphate is presnt on some endolysosomes and lysosomes. This yields clues about how phosphoinositides regulate membrane sorting and biogenesis of different …
-
Binding properties of adaptor proteins Tollip and Tom1
… respectively, whereas Tollip interacts with phosphatidylinositol-3-phosphate (PtdIns(3)P)-containing endosomal membranes. Tom1 and Tollip interaction and association with endosomes is proposed to be involved in the lysosomal degradation of polyubiquitinated cargo. Through cellular, …
-
Domain-based Bioinformatics Analysis and Molecular Insights for the Autoregulatory Mechanism of Phafin2
… autoinhibit the PH domain binding to membrane phosphatidylinositol 3-phosphate (PtdIns3P). Firstly, the study investigated the domain-based evolutionary pattern of PH, FYVE, and polyD motif of Phafin2 among its orthologs and Phafin2- like proteins. Using different bioinformatics tools and …
-
Molecular mechanisms of exosome secretion
… secretion. We found that the conversion of phosphatidylinositol-3-phosphate (PI(3)P) to phosphatidylinositol-4-phosphate (PI(4)P) catalyzed by Myotubularin 1 (MTM1) and phosphatidylinositol 4-kinase type IIα (PI4KIIα) on the surface of MVEs mediates the recruitment of the exocyst complex. …
-
How Oomycete and Fungal Effectors Enter Host Cells and Promote Infection
… RXLR and RXLR-like motifs are able to bind phosphatidylinositol-3-phosphate (PtdIns- 3-P) to mediate entry into host cells. The use of novel inhibitory mechanisms has shown effector entry can be blocked either by sequestering PtdIns-3-P on the outer leaflet of plant and animal cells or by …
-
Activation of lipid kinase VPS34 by Rab GTPases
VPS34 is a lipid kinase that uses phosphatidylinositol (PI) as a substrate to produce the signalling lipid phosphatidylinositol-3-phosphate (PI(3)P). In the cell, VPS34 has to team up with other proteins in order to increase its enzymatic activity. It forms primarily two hetero-tetrameric …
-
Regulation of Traffic into and out of the Yeast Endosome by the VPS9P Cue Domain and the VPS5P Domain
… with Vps26p, Vps29p and Vps35p and the lipid phosphatidylinositol-3-phosphate to recycle the receptor. The significance of these protein-protein interactions and the lipid-protein interaction in Vps5p function is examined. The second question addressed is the regulation of traffic into the …
-
Investigations into the Nature of the Endosomal System in Plasmodium falciparum
… localized the early endosome phosphoinositide, phosphatidylinositol 3-phosphate, to the food vacuole membrane, and in conjunction with our studies on PfFCP, this has raised doubts about the food vacuole as a lysosome equivalent in the parasite. The combination of both early and late endosome …
-
Characterisation of Novel Rab5 Effector Proteins in the Endocytic Pathway
… within a spatially restricted domain enriched in phosphatidylinositol-3 phosphate PI(3)P a complex network of effectors, including PI3-Kinase (PI3-K), the FYVE-finger proteins EEA1 and Rabenosyn-5 that functionally cooperate in membrane transport. Moreover, Rab5 regulates endocytosis from the …
-
Cryo-EM structural exploration of human VPS34 complexes and their activation by RAB GTPases and NRBF2
… kinase that catalyses the conversion of lipid phosphatidylinositol (PI) into phosphatidylinositol-3-phosphate (PI3P) on membranes. The reaction product, PI3P, is a potent signalling lipid involved promoting autophagy, phagocytosis, and endocytic sorting in mammalian cells. In addition to its …
-
Novel lipid regulation of vacuolar fusion in saccharomyces cerevisiae
… vacuoles accumulate the glycerophospholipid phosphatidylinositol 3-phosphate (PI3P). PI3P is a positive regulatory of fusion that functions through the recruitment of multiple components of the vacuolar fusion machinery including the soluble SNARE Vam7. Vam7 interacts with PI3P through its PX …