Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"phosphatidic acid (PA)"”.
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Interactions of Inorganic Mercury and Inorganic Cadmium with Biomimetic and Complex Biological Membranes and their Influence on Membrane Packing and Size
… and anionic lipids respectively. Hg2+ also imparts rigidity by acting as a catalyst in the vinyl ether hydrolysis of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) plasmalogens. Cd2+-induced rigidity of anionic membranes results in a stabilization of the gel phase and a suppression …
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D2R Dopamine Receptor Mediates Changes in Dual Specificity Phosphatase Expression in a Small Cell Lung Cancer Cell Line
<p>Bromocriptine, a D2 dopamine receptor (D2R) agonist, is used clinically as a treatment for pituitary tumors of a lactotroph origin. Many questions remain unanswered about the mechanism of this effect. The antiproliferative effect has not been demonstrated in DMS 53 cell line, a Small Cell Lung …
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The role of the Dgk1 and Pah1 in lipid interconversion on saccharomyces cerevisiae vacuole physiology, homeostasis, protein sorting and fusion
… and regulatory lipids. The deletion of the phosphatidic acid (PA) phosphatase gene Pah1, which converts PA in diacylglycerol kinase (DAG), was previously shown to produce a fragmented vacuole phenotype, greatly inhibit fusion and result in the vacuolar protein sorting defect. As such, the …
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Nanoscale Organization of The Small Gtpase Rac1
… by a C-terminal membrane anchor that encompasses a geranylgeranyl-cysteine-methyl-ester, palmitoyl, and a polybasic domain (PBD) of contiguous lysine and arginine residues. Using high-resolution imaging combined with spatial mapping analysis, I found that Rac1 forms nanoclusters on the PM. …
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Biochemical and physiological studies of Arabidopsis thaliana Diacylglycerol Kinase 7 (AtDGK7)
… the substrate diacylglycerol (DAG) to generate phosphatidic acid (PA) . Both molecules, DAG and PA, are involved in signal transduction pathways. In the model plant Arabidopsis thaliana, seven candidate genes (named AtDGK1 to AtDGK7) code for putative DGK isoforms. Here I report the molecular …
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Regulation of RhoA Activation and Actin Reorganization by Diacylglycerol Kinase
Rho GTPases are critical regulators of actin cytoskeletal dynamics. The three most well characterized Rho GTPases, Rac1, RhoA and Cdc42 share a common inhibitor, RhoGDI. It is only recently becoming clear how upstream signals cause the selective release of individual Rho GTPases from RhoGDI. For …
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The role of phospholipase D1 in trafficking and processing of amyloid precursor protein
… protein (APP) with major implications for pathological and behavioral manifestations associated with Alzheimer's disease (AD). One such lipid is phosphatidic acid (PA), which mediates membrane trafficking and is produced by a variety of enzymes, including phospholipase D (PLD). We …
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Phospholipase D Signaling in T Cells
… T lymphocytes induces the activation of phospholipase D (PLD) signaling. Phospholipase D (PLD) is a phosphodiesterase that catalyzes the conversion of phosphatidyl choline (PC) to phosphatidic acid (PA). PA is an important lipid second messenger and is known to mediate a variety of cellular …
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Beteiligung der Phospholipase D an der Regulation der Synthese von Phosphatidylinositol-4,5-bisphosphat durch Arf- und Rho-GTPasen
The reaction product of phospholipase D (PLD), phosphatidic acid (PA), was found to stimulate phosphatidylinositol-4-phosphate-5-kinase (PIP-5-kinase) activity in vitro. In the present study, we have examined wether PLD affects the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) by …
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Identifying the Role of Phospholipase D1 and Phosphatidic Acid in Exocytosis
… can assist these processes. One protein in particular, phospholipase D1 (PLD1), and its product, the lipid phosphatidic acid (PA), are involved in multiple exocytic processes. However, PLD1 and PA’s role in this process has remained unclear. In this work, PLD1 and the production of PA were …
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The Ligand and Membrane-binding Behaviour of the Phosphatidylinositol Transfer Proteins (PITPa & PITPb)
… to be lipid transfer proteins based on their capacity to transfer either phosphatidylinositol (PI) or phosphatidylcholine (PC) between membrane compartments in vitro. In Drosophila, the PITP domain is found to be part of a multi-domain protein named retinal degeneration B (RdgBα). The PITP …
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The Regulation of Lipid Metabolism and Mitochondrial Quality Control in Health and Disease
… years. As such, it is critical to understand the pathogenesis of disease where aging is the main risk factor. While not widely known, age is in fact a large risk factor in development of obesity and metabolic syndrome. More widely known and discussed are the neurodegenerative diseases that occur …
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The Role of Arabidopsis thaliana Annexins 1, 2 and 4 in Extracellular ATP Signalling
… oxygen species (ROS), nitric oxide (NO) and phosphatidic acid (PA). The identification of $\it{Arabidopsis}$ $\it{thaliana}$ AtDORN1 (Does Not Respond to Nucleotides) as the first higher plant purinoreceptor has confirmed eATP in plant signalling systems. AtDORN1 is a plasma membrane receptor …
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An Investigation into the Function of CDS2 in Phosphoinositide Synthesis in Murine Macrophages
… as Phosphoinositide 3-kinase (PI3K) and Phospholipase C (PLC) signalling pathways, the definition of sub-cellular membrane identity and the sorting of proteins and lipids within intracellular compartments. GPCR-stimulated PLC generates the second messengers inositol (1,4,5)-trisphosphate and …
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Regulation of phosphatidylcholine biosynthesis in apium graveolens
… presence of the sterol biosynthesis inhibitor, paclobutrazol, suspension cultures of Apium graveolens (celery) accumulate substantial amounts of I 4a-methylsterols, at the expense of 4-demethylsterols. These changes have been correlated with reduced synthesis of phosphatidylcholine (PC) via the …
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Innovations in detecting food fraud using mass spectrometric platforms and chemometric modelling
… of food, food ingredients and food packaging for an economic gain. It is in many cases close to impossible for consumers and players in the food industry to identify. The aim of this thesis was to study whether mass spectrometric platforms, especially ambient mass spectrometry (AMS) …
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The use of enzymes, isomerisable lipid analogues and small molecules to modulate the structure and dynamics of lipid bilayer
… Phase Behaviour – The phase behaviour of 1,2-dipalmitoyl-sn-glycerol-3-phosphocholine-d62 (d62-DPPC) samples containing cholesterol and the serotonin receptor agonist drugs quipazine and LY-165,135 was analysed using proton and deuterium NMR spectroscopy and differential scanning calorimetry …
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The regulation of yeast homotypic vacuole fusion by phosphatidic acid and diacylglycerol
… homeostasis. Ultimately, two membrane compartments are brought to each other, direct contact is established, and the bilayers and lumenal contents mix. In Saccharomyces cerevisiae, homotypic vacuole fusion occurs through a series of well-defined phases. SNAREs are activated by their …
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Novel lipid regulation of vacuolar fusion in saccharomyces cerevisiae
… is the membrane fusion of two distinct compartments leading to the mixing of contents. In order to regulate this process organelles are enriched with specific fusion proteins termed SNAREs. While the core fusion machinery is well understood, relatively little is known about the role lipid …
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The role of a monomer/dimer equilibrium and the C-terminal tail in regulating translocation of sphingosine kinase 1 to the plasma membrane of breast cancer cells
… Treatment of cells with either the phospholipase D (PLD) inhibitor, 5-Fluoro-2-Indolyl des-chlorohalopemide (FIPI), or the Gq inhibitor, YM254890 reduced translocation in response to phorbol myristate acetate (PMA), carbachol or S1P, suggesting that phosphatidic acid (PA) formation and Gq …
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