Global ETD Search
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Showing 1 to 17 of 17 for “"phorbol myristate acetate"”.
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Functional characterization of equine neutrophils in response to calcium ionophore A23187 and phorbol myristate acetate
… with calcium ionophore A23187 (A23187) and phorbol myristate acetate (PMA). These stimulants trigger different branches of the PMN activation process that occurs in vivo. Equine PMN were isolated from the whole blood of six clinically normal geldings using a one-step discontinuous Percoll …
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Somatolactogenic hormones and interferon-gamma as regulators of neutrophil activation
… anion (O$\sb2\sp-$) after stimulation with phorbol myristate acetate (PMA). GH, as well as its growth promoting peptide, insulin-like growth factor-I (IGF-I), were both comparable to IFN-$\gamma$ in augmenting the secretion of O$\sb2\sp-$ by PMN. These results indicated that the IGF-I …
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The role of a monomer/dimer equilibrium and the C-terminal tail in regulating translocation of sphingosine kinase 1 to the plasma membrane of breast cancer cells
… to the PM in MCF-7L breast cancer cells. Phorbol myristate acetate (PMA) induced the extracellular signal-regulated kinase (ERK)-dependent translocation of wild type (WT) mouse green fluorescent protein (GFP)-SK1 (mGFP-SK1) from the cytoplasm to lamellipodia in the PM of MCF-7L breast …
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Amiodarone-induced pulmonary toxicity in F344 rats
… rats at day 3 produced more integrated phorbol-myristate-acetate stimulated luminol-dependent chemiluminescence over 20 minutes than BAL cells from control rats. These findings indicate that this model exhibits a transient pulmonary inflammation, with the potential for elevated oxidant …
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A cell-based sensor of fluid shear stress for microfluidics
… and clonal selection. Inducing the pathway with Phorbol-myristate-acetate resulted in fluorescence induction by both microscopy and flow cytometry, verifying the sensor functionality. The fluorescence activation was then characterized across PMA doses and durations. Next, the sensors were tested …
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Regulation of the Gene Encoding Thrombin-Activable Fibrinolysis Inhibitor in Non-Hepatic Cells
… describe the effect of external stimuli such as phorbol myristate acetate (PMA) on CPB2 expression in Dami and THP-1 cells. We found that PMA treatment increases both CPB2 mRNA abundance and promoter activity in Dami cells, and decreases both CPB2 mRNA abundance and promoter activity in THP-1 …
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The desmoplastic response : mechanisms of tumour-induced fibrogenesis
… retinoic acid and the tumour promoter, phorbol myristate acetate, had significant primary effects on fibroblast collagen synthesis but did not modify the response to melanoma cells. Indomethacin, however, had a minimal primary effect upon the fibroblasts but significantly augmented the …
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Establishment of a Long Term Cell Culture Model for Testing Anti-Infectives against Mycobacterium avium subsp. paratuberculosis
… of retinoic acid (RA), vitamin D (VD), and phorbol myristate acetate (PMA) in combination in cell culture, we were able to screen novel therapeutics before embarking on in vivo testing in animals. This is a significant step forward in Crohn's and Johne's disease treatment research. We are …
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Protein phosphorylation and cell diversification in the mouse early embryo.
… two drugs, 6-dimethylaminopurine (DMAP) and a phorbol ester (phorbol myristate acetate, PMA), each of which seems to affect both protein phosphorylation and compaction. DMAP is an adenine analogue and putative inhibitor of protein phosphorylation that was found to perturb the cell cycle of …
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Effects of taurine and hypotaurine on oxidative lung injury
… taurine was found to augment H₂O₂ production in phorbol myristate acetate-stimulated macrophages. At 26, 78, and 104 mM, taurine was found to elevate H₂O₂ production 13%, 28%, and 43%, respectively, above the positive control. Taurine (5-120 mM) and hypotaurine (2-10 mM) were also ineffective (p …
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Modification of Low Density Lipoprotein and the Effects of Modified Low Density Lipoproteins on the Endocytic and Secretory Repertoire of the Macrophage
… x 10<sup>6</sup> cells per ml), with or without phorbol myristate acetate (100 ng/ml) to trigger the respiratory burst, did not oxidize LDL in the TBA assay, despite producing large amounts of reactive oxygen intermediates. EDTA-LDL, CDP-LDL, and acetoacetylated LDL failed to trigger …
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Characterising the effects of 1,4,5-Oxathiazinane-4,4-dioxide (OTD) on non-neoplastic cells and primary and metastatic melanoma cells in vitro
… of IFNγ release in cells stimulated with phorbol myristate acetate (PMA)/ionomycin or anti-CD3, at all concentrations of OTD, despite preservation of cell viability (n=3, p <0.05). Considering the infiltration of CD8+ T lymphocytes into the surgical site approximately one week …
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In vivo and in vitro regulation of plasminogen activator inhibitor type-1
… the megakaryoblastic pathway by the addition of phorbol myristate acetate and, during this process, the cells switch from producing a mixture of active tissue plasminogen activator (t-PA) and urokinase (u-PA) to producing active u-PA alone. I performed a series of experiments designed to examine …
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The role of sphingosine kinases and dihydroceramide desaturase in cell survival/apoptosis and inflammation pathways
… SK2 inhibitors, ABC294640 or K145 reduced phorbol myristate acetate (PMA) stimulated phosphorylation of JNK and ERK-1/2 and transcriptional activity of AP-1. In contrast, other SK inhibitors including PF-543, SKi and ROMe did not inhibit JNK and ERK-1/2 signalling and only produced a …
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Characterization of Phagosome-Lysosome Fusion in Macrophages
… agent. Treatment with the tumor promoter, phorbol myristate acetate (PMA) caused a striking increase in the rate and extent of P-L fusion. Exposure of cells to phorbol, free myristate, or the monoesters of PMA did not reproduce this effect. Macrophages required from 2 - 3 hr of pretreatment …
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Phosphorylation sites on specific neuronal proteins can control the mode of synaptic vesicle exocytosis and thereby regulate synaptic transmission
… of PKC as demonstrated using a PKC activator, phorbol myristate acetate, and an inhibitor, Go 6983. Therefore, endogenous PKC activation may phosphorylate this site when a very high Δ[Ca2+]i is achieved at the active zone. It was difficult to obtain a conclusion as to which PKC isoform could …
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Role of Vitamin-D3 and Retinoic Acid In A Human Thp-1 Macrophage Model of Mycobacterium Tuberculosis Infection
<p>Mycobacterium tuberculosis (Mtb) replicates within the human macrophages and we investigated the activating effects of retinoic acid (RA) and vitamin D3 (VD) on macrophages in relation to the viability of Mtb. A combination of these vitamins (RAVD) enhanced the receptors on THP-1 macrophage …