Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 30 for “"phase-contrast microscopy"”.
-
Corneal fibrosis therapies and investigations in the canine corna in vitro
PHASE 1: ABSTRACT Purpose: To evaluate the safety and anti-fibrotic effects of the drugs, angiotensin-converting enzyme inhibitor (ACE-I) lisinopril and rho-kinase inhibitor (ROCK-I) fasudil, alone and in combination, on the canine cornea using an established in vitro model. Methods: To test the …
-
Characterisation of the Rho GTPase target Dishevelled Associated Activator of Morphogenesis 1 (DAAM1)
… patches in HeLa cells which appeared dark under phase-contrast microscopy. These phase-dark patches co-stained with markers of lipid rafts and stable microtubules. When DAAM1 was knocked down in COS-7 cells in wound healing assays, Golgi/MTOC re-orientation towards the wound edge was impaired. We …
-
Detecting cells and analyzing their behaviors in microscopy images using deep neural networks
… event detection and its stage localization in phase-contrast microscopy images”--Abstract, page iii.</p>
-
Meiosis in Thermobia domestica Pack
… staining with acetic orcein and the use of phase contrast microscopy simplified preparatory procedures and preserved the greatest amount of cell integrity. Photomicrographs taken of the complete meiotic cycle in the male indicated 34 chromosomes in reduction division -- 32 autosomes + X₁X₂. …
-
Spatial light interference microscopy and applications
Phase contrast microscopy has revolutionized cell biology by rendering detailed images from within live cells without using exogenous contrast agents. However, the information about the optical thickness (or phase) is qualitatively mixed in the phase contrast intensity map. Quantifying optical …
-
Kinetics of Nesquehonite Nucleation
… as demonstrated by the direct observation using phase-contrast microscopy.</p>
-
The inhibition of germination and growth of Clostridium botulinum 62A by BHA, BHT, TBHQ and 8-Hydroxyquinoline
… (600 nm) decrease or by increase in percent phase dark spores viewed under phase contrast microscopy. Strict anaerobic conditions were maintained throughout the study. As the concentration of each chemical was increased the rate of germination or growth decreased. This occurred for each pH …
-
Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
… different CB concentrations were assessed via phase contrast microscopy, live/dead staining and Alamar Blue® staining respectively. The results show that both 2D and 3D of ORL-48 microtissues were only morphologically affected (fibroblastic spreading to round shape) while cell viability and …
-
In vitro investigation of the molecular relationship between mitochondrial dysfunction and neurogenesis in autism aetiology
… was morphologically quantified using phase-contrast microscopy and the ‘Neuron J’ plugin in Image J. Neuronal differentiation was also biochemically confirmed using immunocytochemistry for the 5 immature neuron marker, Nestin, in comparison to the mature neuron marker, Beta-III …
-
Detection and tracking of cells and focal adhesions in microscopy images
… of cell function and structure. Modern microscopy and imaging techniques have enabled cell biologists to observe and record cell processes in detail, thus generating high volumes of imaging data. Meaningful conclusions can only be drawn from such big datasets using automated quantitative …
-
Effects Of Cholesterol On Bovine Sperm Survival During Cooling and Freezing
… factor is cholesterol, which decreases the phase transition of membrane lipids that occurs during the cooling and freezing process. One known method of membrane cholesterol enrichment employs cholesterol loaded cyclodextrin (CD/CHOL). The purpose of this study was to confirm current CD/CHOL …
-
Studies on the effects of sub-minimal inhibitory concentrations of antibiotics on the virulence factors of biofilm bacteria
… These results were further substantiated in phase contrast microscopy images showing advanced stages of oncosis in tissue cultured mouse spleen myeloma (Sp2) cells treated with leucocidin isolated from <i>Ps. aeruginosa</i> treated with sub-MIC of<br/>ampicillin (8 pg mL'1).<br/><br/>The …
-
The Effect of Different Dietary Fats on The Lipid Composition of Swine Cardiac Plasma Membrane
… Ca('++),K('+)-ATPase activities. Electron and phase contrast microscopy showed that the isolate was relatively free of contractile proteins and contaminating structures. The yield of isolate protein to that of the initial homogenate was approximately 7% per preparation. Lipids were extracted …
-
Investigating the cellular toxicology of silver nanoparticles using a single-cell, mitosis-focused approach
… of 6 weeks (chronic treatment). Time-lapse, phase-contrast microscopy of acutely treated cells showed that 100% of cells engulfed AgNPs, which was further confirmed by electron microscopy. Moreover, we found that higher concentrations of AgNPs resulted in large numbers of acutely treated …
-
Mathematical Imaging Tools in Cancer Research - From Mitosis Analysis to Sparse Regularisation
… In cancer drug development, time-lapse light microscopy experiments are conducted for performance validation. The aim is to monitor behaviour of cells in cultures that have previously been treated with chemotherapy drugs, since atypical duration and outcome of mitosis, the process of cell …
-
Chip-scale diffraction phase microscopy
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-05-01
-
Real-time quantitative phase imaging for cell studies
… Several methods have been developed to improve contrast in cell imaging, including use of exogenous contrast agents such as fluorescence microscopy, as well as utilizing properties of light-specimen interaction for optics design, to reveal the endogenous contrast, such as phase contrast …
-
Bioadhesion to model thermally responsive surfaces
… were grown on the mixed SAM and imaged by phase contrast microscopy to show inhibition of attachment at temperatures below the molecular transition. Qualitative and quantitative measurements of the fibroblast adhesion data are provided that support the hypothesis of the mixed SAM exhibiting …
-
Investigating the putative therapeutic effects of sulfophane in the human lens
… software. Capsular bags were monitored using phase-contrast microscopy. The ApoToxGlo Triplex assay was used to assess FHL 124 cell survival, cytotoxicity and apoptosis. The MTS assay was used to assess cell populations. To determine levels of DNA strand breaks, the alkaline comet assay was …
Page 1 of 2