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Showing 1 to 20 of 28 for “"phalloidin"”.
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Synthese und Cytotoxizität membranpermeabler Phallotoxine
… zu 1000-mal höher waren als die des natürlichen Phalloidins. Die Cytotoxizität wurde anhand der Proliferationshemmung in Mausfibroblasten-Kulturen bestimmt, wobei für Oleoyl-Phalloidin, Tat-SS-Phalloidin und Poly-L-Lysin-Phalloidin IC50-Konzentrationen von 2.5 µM, 3.8 µM bzw. 3.1 µM erhalten …
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Two-Color Fluorescent Analysis of Connexin 36 Turnover and Trafficking – Relationship to Functional Plasticity
… in Cx36 vesicle trafficking and GJ mobility. Phalloidin labeling showed that thick actin bundles connected all edges of GJ plaques, but actin filaments were rare within. Actin filaments were found associated with small, chase-labeled delivery vesicles. Many GJs showed substantial numbers of …
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A Molecular Basis for Divergent, Unstable Actin Filaments in Toxoplasma gondii
… using molecular modeling and biochemical assays. Phalloidin binding to parasite actins rescued the short filaments demonstrating that although TgACTI naturally forms short, unstable filaments on its own, it is capable of forming longer, conventional filaments when stabilized. Molecular docking was …
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Analyzing GFP-tagged cytoskeletal protein colocalization in human carcinoma cells
… in three channels-Hoechst for nucleus, Texas Red phalloidin for actin, and GFP for protein-and images were captured using Cellomics scanning microscopy. After collecting data and testing software applications, we analyzed our data with Definiens software and developed a flexible, comprehensible …
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Intracellular pH, the Proximate Signal for Cell Volume Changes that are Mediated by the Actin Cytoskeleton
… Treating TRCs with cytoskeleton inhibitors, phalloidin and cytochalasin, before acidic stimulation did not affect TRC pHi, but did result in an altered TRC volume change. I conclude that a decrease in TRC pHi induces cell shrinkage via the actin cytoskeleton. Cell shrinkage as a result of a …
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An in-vitro investigation to determine the neuroinflammatory response of CNS cells to oral bacteria and their virulence factors.
… responses were examined using fluorescein-phalloidin labelling and recorded using the confocal microscope. Binding of two specific endotoxins to cells were determined by immunolabelling with anti-P. gingivalis (clones 1B5, and 1A1) antibodies to LPS, and its specific receptor CD14, and …
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The effects of membrane and cytoskeletal mechanics on cell adhesiveness
… we treated cells with the pharmacological agents phalloidin, Latrinculin B, Cytochalasin D, colchicine, and Paclitaxel, which have varying effects on microfilaments and microtubules, two of the main constituents of the cytoskeleton. We found that dissolution of the actin cytoskeleton could reduce …
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The Time-Course of the Effects of Growth Hormone During Zebrafish (<i>DANIO RERIO</i>) Auditory Hair Cell Regeneration
… dB re 1 μPa root mean squared (RMS) for 36 h. Phalloidin-staining was used to assess the effects of GH on hair cell bundle density; BrdU-labeling was used to assess the effects of GH on cellular proliferation; TUNEL-labeling was used to assess the effects of GH on apoptosis in the zebrafish …
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Untersuchungen zur Wirkung des Pasteurella multocida Toxins auf die endotheliale Barrierefunktion in vitro
… Zytoskeletts wurden die Zellen mit Rhodamin Phalloidin gefärbt und im Fluoreszenzmikroskop analysiert. Mit Hilfe spezifischer Hemmstoffe, die teilweise in die Zellen mikroinjiziert wurden, sowie der biochemischen Messung von Enzymaktivitäten bzw. Phosphorylierung, erhielten wir folgenden …
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Environmental influences on the development of the female honeybee brain Apis mellifera
… mittels einer Doppelfluoreszenzfärbung mit Fluor-Phalloidin und anti-Synapsin Immuncytochemie durchgeführt. Diese Doppelmarkierung ermöglichte die Visualisierung und Quantifizierung individueller Synapsenkomplexe (Microglomeruli) im Pilzkörper-Calyx. Phalloidin bindet an verschiedene F-Aktin …
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Der Einfluss von Yersinia-Effektoren auf podosomale Adhäsionsstrukturen primärer humaner MAkrophagen
… infiziert. Nach Färbung der Zellen mit Rhodamin-Phalloidin wurde die Anzahl der verbliebenen Zellen mit Podosomen im konfokalen Mikroskop ermittelt und statistisch ausgewertet. Es konnte erstens gezeigt werden, daß ein voll virulenter Yersinien Stamm in der Lage ist, nach einer Infektion von …
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Lithograph regulation of cellular mechanical properties c by Tsu-Te Judith Su.
… plating, the cells were fixed and stained with phalloidin. A high-speed, two-photon scanning microscope was used to resolve actin architecture in three dimensions and a fractal dimension measurement was performed to quantify the distribution of actin within the cell as a function of adhesion …
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Auditory Hair Cell Regeneration and Gene Expression in Noise-Exposed Zebrafish (Danio rerio)
… cell loss and recovery were determined using phalloidin and DAPI labeling to visualize hair cell bundles and nuclei, respectively. Cell proliferation was quantified through BrdU labeling. Immediately following noise exposure, zebrafish saccules exhibited significant hair cell bundle loss and …
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Frazzled And Abelson Interact To Regulate The Actin Cytoskeleton In Drosophila
… on concanavalin A coated cover slips. These phalloidin stained filopodia are reduced when either the P1 or P3 cytoplasmic motif is removed. Frazzled is localized to the tips of these filopodia, and is associated with an increase in phosphotyrosine levels as well as alterations in localization …
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Gingko biloba extracts protect auditory hair cells from cisplatin-induced ototoxicity by inhibiting perturbation of gap junctional intercellular communication
… hair cells was counted by co-labeling with phalloidin and MyoVIIa. EGb prevented cisplatin-induced apoptosis in immunostaining and decreased caspase 3 and poly-ADP- ribose polymerase (PARP) bands, which were increased in cisplatin-treated cells in Western blots. EGb prevented abnormal …
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The Effects of Growth Hormone in the Inner Ear of Zebrafish (<i>Danio rerio</i>) during Hair Cell Regeneration
… assay. Hair cell regeneration was determined by phalloidin-labeling to allow visualization of hair cell stereociliary bundles. After GH injection, the numbers of TUNEL-labeled cells showed a significant decrease in all three inner ear end organs (saccule, lagena, utricle), suggesting GH may …
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Reconstitution of a Multi-layered, Differentiated Cornea by HTERT-Immortalized Corneal Epithelial Cells Transduced with Thymidine Kinase Transplanted onto Denuded Mouse Corneas
… 3, Ki-67, and the fluorescent probes – PI, and Phalloidin. Cytotoxicity of ganciclovir was assessed at concentrations of 0.1, 0.5, 1.0, 5.0, and 10.0 μM using Live-Dead Assay. RESULTS: IF confirmed an intact corneal basement membrane following epithelial removal. At 4 hours and 1 day …
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Estudo da modulação da migração de células hospedeiras induzida por Leishmania e seu impacto na disseminação do parasito no hospedeiro
… the expression of Rac1, Rho-A, Cdc42, and phalloidin staining in these cells, as well as the formation of podosomes by immunostaining vinculin. Finally, we investigated the role of the AKT/PI3K signaling pathway in the migration of host cells infected by these parasites, through the …
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Estudo do papel do lpg2 na migração de células dendríticas infectadas por Leishmania infantum
… formation; and Rac1, Cdc42, Gelsolin, RhoA, and phalloidin to evaluate actin polymerization using confocal microscopy. Furthermore, the expression of CCR7 in dendritic cells infected by different Leishmania strains was assessed by flow cytometry. Finally, to investigate the activation of the …
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STUDIES ON A CALCIUM-DEPENDENT ACTIVATOR PROTEIN, STRUCTURALLY AND FUNCTIONALLY HOMOLOGOUS TO CALMODULIN, FROM THE WATER MOLD ACHLYA AMBISEXUALIS RAPER.
… growing hyphae are insensitive. Rhodamine-phalloidin or anti DNAse I-stained F-actin structures observed in Achlya include peripheral plaques (near the hyphal tips), cytoplasmic filaments and intranuclear structures. Differentiating sporangia have uniformly distributed actin plaques. …
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