Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"phagosomes"”.
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Proteomic analysis of the Mycobacterium avium-containing phagosome membrane
… avium (M.av), reside intracellularly in phagosomes where they are able to survive, because they block the process of phagosome maturation. ... An approach of characterizing M.av-containing phagosomes in terms of cell surface-derived glycoconjugates, revealed that these phagosomes show a …
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Phagocytosis by neutrophils - studies on phagosome dynamics and membrane traffic modulation by Streptococcus pyogenes
… organelle, the phagosome. Neutrophil phagosomes are highly dynamic entities, and a large amount of antimicrobial substances are released to their interior within seconds of formation. In most cases this will kill the engulfed microbe, but there are exceptions. Streptococcus pyogenes is …
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Novel Mechanisms of Antigen Processing That Enhance Bcg Vaccine Efficacy
… BCG vaccine affect antigen processing within phagosomes. These studies led to the discovery that a mutant of BCG vaccine which lacked SOD was a better vaccine. Subsequently, the proteomic analysis of vaccine phagosomes led to the discovery of novel protease (γ-secretase) enriched on BCG …
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MyTH4 and FERM Have Overlapping and Distinct Roles in the Function of Myo1, a Class XIV Myosin in Tetrahymena thermophila
… studies have shown that Myo1 localizes to phagosomes, the cytoskeleton, and the macronucleus, and that phagosome trafficking and division of the macronucleus are affected in a MYO1 knockout. To investigate the roles for MyTH4 and FERM in the function of Myo1, GFP-tagged MyTH4, FERM, and …
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Phagosome proteomes in activated macrophages
… such as microbes or apoptotic cells to form phagosomes which then fuse with endosome and finally lysosomes, where the particles are finally degraded. My project aims at investigating the phagosome functions regulated in activated macrophages and further exploring the mechanism by which …
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Ubiquitylation regulates vesicle trafficking and innate immune responses on the phagosome of inflammatory macrophages
… and immune signalling. <br/>My data shows that phagosomes are enriched in polyubiquitylation, which is further enhanced by IFN-ɣ. I applied a targeted AQUA peptide approach by which we quantified ubiquitin chain linkage peptides from phagosome samples by PRM. This data shows that all chain …
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Deciphering the regulation of perforin-2-mediated endocytic escape in dendritic cells
… perforin-2 was recruited to antigen-containing phagosomes, I studied its stepwise proteolytic processing during phagosome maturation. Flow cytometry analysis of isolated phagosomes revealed that, in line with previous *in vitro* studies, perforin-2 processing was dependent on acidification. …
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Membrane Surface Charge and Lipid Signaling in Phagocytosis
… innate and adaptive immunity. The formation of phagosomes and the subsequent maturation that capacitates them for pathogen elimination and antigen presentation are complex processes that involve signal transduction, cytoskeletal reorganization and membrane remodeling. Lipids are increasingly …
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Transcriptomic and Proteomic Studies of Intercellular Communication Between Melanocytes and Keratinocytes in Human Skin
… used a proteomic approach to study keratinocyte phagosomes. This is the first such proteomic characterization of phagosomes from an epithelial cell. In the final chapter of my thesis, I outlined the immediate future directions of this work, and discussed how these basic biological findings may be …
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Regulation of Myosin V-Cargo Interactions by Phosphorylation
… Similarly, myosin V recruitment to mouse phagosomes during phagocytosis is blocked by PAK1 phosphorylation. Thus, the binding of myosin V to cargo is regulated by phosphorylation of the tail domain, and may represent a common mechanism of regulation of vertebrate myosin V.
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Analysis of the maturation of Rhodococcus equi-containing vacuoles in macrophages
… that R.equi was found equally frequently in phagosomes with loosely or thightly apposed membranes, and RCV often contains numerous membranous vesicles. Laser scanning microscopy of infected macrophages showed that the majority of phagosomes containing R.equi acquired transiently the early …
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Induction of Stronger and Long Lasting Vaccine Immunity to Tuberculosis
… tuberculosis. BCG vaccine localizes to immature phagosomes of macrophages, and avoids lysosomal fusion, which decreases peptide antigen production. Peptides are essential for macrophage-mediated priming of CD4 and CD8 T cells respectively through MHC-II and MHC-I pathways. Furthermore, BCG …
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Morphology and Localisation of Patient Hip Replacement-Derived Debris and Hip Prostheses Materials in Macrophages
… for these materials in vitro, localising to phagosomes or in the cytoplasm, sometimes in gross quantities. Titanium formed needle-shaped particles which increased apoptosis and altered macrophage appearance. Surface characteristics varied for patient particles, further concluding the …
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Microbiology and cell biology of the interaction between Listeria monocytogenes and Acanthamoeba spp.
… of mammalian cells, it could not escape from phagosomes and evade the killing mechanisms of amoeba trophozoites. Consequently, bacteria are killed within phagolysosome in trophozoites before they express their virulence genes to escape from phagosomes and get access to cytoplasm. This was …
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Effect of cellular factors on the generation of beta-amyloid.
… expressing the fusion protein reveals numerous phagosomes and mutilaminar bodies that fit the profile seen for the blob-like aggregates in terms of dimension, number and general morphology. These data suggest that the blob-like aggregates might be novel membrane-bound structures. These fusion …
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Investigating The Role of Quaking In Antigen Uptake and Cross-Presentation By Dendritic Cells
… have identified QKI as a novel regulator of phagosomes and endolysosomes, and knocking down of QKI significantly downregulates genes involved in phagosome maturation and endosome signaling. Furthermore, we have also shown that QKI interacts with a nuclear receptor, PPARδ, in multiple cell …
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THE FUNCTIONAL REGULATION OF FCRN EXPRESSION AND FCRN-MEDIATED ANTIGEN PRESENTATION
… MHC II-peptide assembly. However, the alkalized phagosomes in DC failed FcRn to enhance the antigen presentation of ICs.
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Optimization and Validation of the Derivation and Use of HoxB8 Transgenic Murine Phagocytes in In Vitro Studies
… Yates focuses on the microenvironment within phagosomes in macrophages. The research in this area is limited due to the use of bone marrow-derived macrophages that are short-lived, require the constant sacrifice of mice, and are very difficult to genetically manipulate. To overcome these …
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Mild traumatic brain injury augments innate immune responses through neurokinin and cholinergic signaling
… generate oxidative burst, or acidify phagosomes. However, neutrophils from mTBI mice produced more neutrophil extracellular traps in response to bacterial challenge. These studies show that neurokinin signaling in our model contributes to enhanced bacterial clearance. Cholinergic …
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Transcription analysis of virulent strains of Mycobacterium tuberculosis
… use copper to poison bacteria trapped in their phagosomes provides the link to virulence and pathogenicity. Conclusions: Through the integration of data from multiple data types we were able to elucidate the most probable cause of the altered virulence found between the two isolates in this …
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