Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"phagosome maturation"”.
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Deciphering the regulation of perforin-2-mediated endocytic escape in dendritic cells
… perforin-2 was recruited to antigen-containing phagosomes, I studied its stepwise proteolytic processing during phagosome maturation. Flow cytometry analysis of isolated phagosomes revealed that, in line with previous *in vitro* studies, perforin-2 processing was dependent on acidification. …
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Analysis of the maturation of Rhodococcus equi-containing vacuoles in macrophages
… that R.equi was found equally frequently in phagosomes with loosely or thightly apposed membranes, and RCV often contains numerous membranous vesicles. Laser scanning microscopy of infected macrophages showed that the majority of phagosomes containing R.equi acquired transiently the early …
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Proteomic analysis of the Mycobacterium avium-containing phagosome membrane
… avium (M.av), reside intracellularly in phagosomes where they are able to survive, because they block the process of phagosome maturation. ... An approach of characterizing M.av-containing phagosomes in terms of cell surface-derived glycoconjugates, revealed that these phagosomes show a …
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Ubiquitylation regulates vesicle trafficking and innate immune responses on the phagosome of inflammatory macrophages
… a de novo, membranous organelle known as the phagosome. Regulation of phagocytosis and phagosome maturation can be achieved through changes in transcription/translation and differential recruitment of proteins but also through their non-translational modifications. Here I explored the role of …
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Phagosome proteomes in activated macrophages
… by cytokine activation is phagocytosis and phagosome maturation, through which macrophages engulf foreign material such as microbes or apoptotic cells to form phagosomes which then fuse with endosome and finally lysosomes, where the particles are finally degraded. My project aims at …
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Components Required for the Death and Degradation of the Linker Cell in C. Elegans
… their regulators ensure timely phagocytosis and phagosome maturation. I also determined that the caspase CED-3 and its upstream regulator CED-4 are required not for cell death, but for proper cell corpse removal. This new role of caspase in cell corpse disposal offers an alternative function for …
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Investigating The Role of Quaking In Antigen Uptake and Cross-Presentation By Dendritic Cells
… have identified QKI as a novel regulator of phagosomes and endolysosomes, and knocking down of QKI significantly downregulates genes involved in phagosome maturation and endosome signaling. Furthermore, we have also shown that QKI interacts with a nuclear receptor, PPARδ, in multiple cell …
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Tuberculosis transcriptomics: host protection and immune evasion mechanisms
… effector functions which were independent of phagosome maturation and autophagy in primary murine macrophages. Our studies suggested that these novel genes play a role in the immune response to Mtb and should be studied more thoroughly to evaluate their potential in possible TB interventions.
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Early immunity to Mycobacterium tuberculosis: new insights into macrophage heterogeneity and vaccine-mediated responses
… Additionally, a number of genes relating to phagosome maturation and nitric oxide production were also differentially expressed. However, validation studies using gene knockout animals did not support a cell-intrinsic, protective role afforded by these genes. In addition to these host …
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Understanding buruli ulcer : elucidation of the cellular and molecular mechanisms underlying infection with mycobacterium ulcerans
… mechanisms triggered by IFN-γ, such as phagosome maturation, phagolysosome fusion, and generation of reactive nitrogen intermediates. This inhibition of macrophage antimicrobial activity was due to the production of mycolactone, since the addition of increasing concentrations of purified …