Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"phagosome"”.
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Phagosome proteomes in activated macrophages
… by cytokine activation is phagocytosis and phagosome maturation, through which macrophages engulf foreign material such as microbes or apoptotic cells to form phagosomes which then fuse with endosome and finally lysosomes, where the particles are finally degraded. My project aims at …
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Characterization of Phagosome-Lysosome Fusion in Macrophages
… were used to study the determinants of phagosome-lysosome (P-L) fusion in intact mouse macrophages. Lysosomes were labeled with the fluorescent vital dye acridine orange and the rate and extent of their fusion with yeast-containing phagosomes was monitored by fluorescence microscopy. …
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Survival of Salmonella in the macrophage phagosome
Salmonella Typhimurium is a gram-negative facultative anaerobe bacterium capable of causing life threatening disease in humans. Transmitted via the fecal-oral route, S. Typhimurium infection typically results in self-limiting gastroenteritis. However, in immunocompromised patients, Salmonella can …
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Proteomic analysis of the Mycobacterium avium-containing phagosome membrane
… avium (M.av), reside intracellularly in phagosomes where they are able to survive, because they block the process of phagosome maturation. ... An approach of characterizing M.av-containing phagosomes in terms of cell surface-derived glycoconjugates, revealed that these phagosomes show a …
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Antimicrobial Effectors Act Cooperatively to Stress Salmonella in the Macrophage Phagosome
… data suggests that the damage occurred in the phagosome is analogous to that used by the secretary in vitro rather than CRAMP. The effect of superoxide on the Salmonella periplasm in the phagosome was also tested by monitoring the expression of these genes in a sodCI deletion background. Our …
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Phagocytosis by neutrophils - studies on phagosome dynamics and membrane traffic modulation by Streptococcus pyogenes
… target inside a membrane-bound organelle, the phagosome. Neutrophil phagosomes are highly dynamic entities, and a large amount of antimicrobial substances are released to their interior within seconds of formation. In most cases this will kill the engulfed microbe, but there are exceptions. …
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Ubiquitylation regulates vesicle trafficking and innate immune responses on the phagosome of inflammatory macrophages
… a de novo, membranous organelle known as the phagosome. Regulation of phagocytosis and phagosome maturation can be achieved through changes in transcription/translation and differential recruitment of proteins but also through their non-translational modifications. Here I explored the role of …
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Yeast-based vaccine approaches to cancer immunotherapy
… Using a yeast vaccine model, we investigated the phagosome-to-cytosol pathway of cross-presentation. We demonstrate that the rate of antigen release from phagocytosed yeast directly affects cross-presentation efficiency, with an apparent time limit of about 25 min post-phagocytosis for antigen …
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Deciphering the regulation of perforin-2-mediated endocytic escape in dendritic cells
… perforin-2 was recruited to antigen-containing phagosomes, I studied its stepwise proteolytic processing during phagosome maturation. Flow cytometry analysis of isolated phagosomes revealed that, in line with previous *in vitro* studies, perforin-2 processing was dependent on acidification. …
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Analysis of the maturation of Rhodococcus equi-containing vacuoles in macrophages
… that R.equi was found equally frequently in phagosomes with loosely or thightly apposed membranes, and RCV often contains numerous membranous vesicles. Laser scanning microscopy of infected macrophages showed that the majority of phagosomes containing R.equi acquired transiently the early …
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Investigating The Role of Quaking In Antigen Uptake and Cross-Presentation By Dendritic Cells
… have identified QKI as a novel regulator of phagosomes and endolysosomes, and knocking down of QKI significantly downregulates genes involved in phagosome maturation and endosome signaling. Furthermore, we have also shown that QKI interacts with a nuclear receptor, PPARδ, in multiple cell …
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The Interactions of Clostridium Perfringens With Phagocytic Cells
… we showed that C. perfringens could escape the phagosome of J774-33 and mouse peritoneal macrophages. We believe the ability of C. perfringens to survive in the presence of macrophages is due to its ability to escape the phagosome. Using a variety of inhibitors of specific receptors, we …
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Identification of genes involved in macrophage activation and effector functions against intracellular pathogens
… to promote L. monocytogenes escape from the phagosome and to negatively regulate transcription activity of C/EBPβ. In addition, since PKCδ was un-regulated, as shown by microarray and confirmed by RT-PCR, in L. monocytogenes infected C/EBPβ-/- macrophages, it was therefore thought to play a …
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Alternative Carbon Utilization As A Virulence Determinant For Candida Albicans
… to clear pathogens in the harsh environment of a phagosome, but<em> C. albicans, </em>as an adaptable opportunist, is capable of surviving macrophage attack to continue dissemination. The Lorenz Lab and other leading labs in the field have identified that <em>C. albicans </em>rapidly adapts to the …
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Effects of in vivo dexamethasone sodium phosphate on in vitro equine neutrophil function
… Neither phagocytosis, iodination, or phagosome-lysosome fusion was affected by treatment. Hydrogen peroxide production, measured both spectrophotometrically and by flow cytometry, was not affected by treatment. Lysosomal enzyme activity for alkaline phosphatase, acid phosphatase, …
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Membrane Surface Charge and Lipid Signaling in Phagocytosis
… innate and adaptive immunity. The formation of phagosomes and the subsequent maturation that capacitates them for pathogen elimination and antigen presentation are complex processes that involve signal transduction, cytoskeletal reorganization and membrane remodeling. Lipids are increasingly …
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Novel Mechanisms of Antigen Processing That Enhance Bcg Vaccine Efficacy
… that BCG vaccine becomes sequestered in special phagosome compartments of macrophages that do not fuse with lysosomes. Since lysosome fusion is necessary for peptide production and T cell priming leading to protective T<sub>H</sub>1 immunity, we hypothesized that vaccine efficacy is reduced and …
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MyTH4 and FERM Have Overlapping and Distinct Roles in the Function of Myo1, a Class XIV Myosin in Tetrahymena thermophila
… studies have shown that Myo1 localizes to phagosomes, the cytoskeleton, and the macronucleus, and that phagosome trafficking and division of the macronucleus are affected in a MYO1 knockout. To investigate the roles for MyTH4 and FERM in the function of Myo1, GFP-tagged MyTH4, FERM, and …
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Development of Tools to Quantitatively Probe Mycobacterium tuberculosis-induced Host Phagosomal and Signaling Responses
… engulf the bacteria and sequester them in the phagosome. This interaction involves the recognition of Mtb by pattern recognition receptors (PRRs) leading to downstream signaling and cytokine production. While there are several hypotheses about how an infected cell controls Mtb, specific …
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Ethanol Impairs Mechanisms of Macrophage Phagocytosis and Cytokine Production
… in actin recruitment to the site of the phagosome compared control conditions. Prior to actin polymerization, the phagocytic response requires recruitment of the adhesion molecules paxillin and vinculin, forming a structure we term "phagosomal adhesion". Ethanol did not alter paxillin …
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