Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 12 of 12 for “"phagolysosome"”.
-
Polar Body Membrane Breakdown Occurs Independent of Macroautophagy in C. Elegans
… (LAP) drives cell corpse breakdown in phagolysosomes, we studied the clearance of the <em>C. elegans</em> polar body during embryonic development. ATG16L1 has separable roles in regulating LC3 recruitment during macroautophagy and LAP. We demonstrated that the ATG16L1 <em>C. …
-
The Roles of Peroxynitrite and Superoxide in Oxidative Damage to E. Coli
… superoxide and nitric oxide (NO$\cdot)$ in the phagolysosome, may also be toxic by the same mechanism as O$\sb2{-}.$ Our in vivo results indicated that peroxynitrite has the same intracellular targets as superoxide. Of nineteen enzymes tested, only the dehydratase enzymes containing these …
-
Identifying novel survival mechanisms of Mycobacterium Tuberculosis within its host
… in resisting the acidic microenvironment within phagolysosome was investigated. An novel in vitro lysosomal exposure model was also described. Through next generation sequencing, the transcriptome of Mtb was profiled based on this model, and has led to the identification of some novel genes that …
-
Characterization of The Ato Gene Family In Alternative Carbon Metabolism
… that should be inhibited in the normally acidic phagolysosome. We have shown that <em>C. albicans</em> induces germination by neutralizing the phagolysosome. To better understand this process we compared transcript profiles of cells in conditions that promote<em> </em>alkalinization <em>in …
-
Microbiology and cell biology of the interaction between Listeria monocytogenes and Acanthamoeba spp.
… Consequently, bacteria are killed within phagolysosome in trophozoites before they express their virulence genes to escape from phagosomes and get access to cytoplasm. This was confirmed by observing no GFP expression by bacteria carried prfA::gfp construct in co-culture with amoebae …
-
Survival Of Mycobacterium Avium Subspecies Paratuberculosis In The Pol
… Tuberculosis', suggesting the ability to resist phagolysosome fusion, by maintaining association with the early endosomes. Overall, the data confirms map virulence in host human blood leukocytes.
-
The Role of Urease in the Pathogenesis of Edwardsiella ictaluri
… tolerate or alter the acidic environment of the phagolysosome. Growth curves performed at acidic pH and survival assays following extreme acid shock indicate that E. ictaluri is naturally acid resistant and is able to utilize urea to enhance growth and replication at acidic pH by the …
-
Analysing M. avium-macrophage interactions and establishing a long-term infection in a novel and realistic model of lung alveolar macrophages, Max Planck Institute Cells
… responses via mechanisms such as preventing phagolysosome fusion and acidification which allows it to proliferate intracellularly thus assisting MAC in the spread of infection. It is vital that these mechanisms are investigated so that the disease process is better understood, and therapeutic …
-
The Membrane Polypeptides of the Vacuolar System: Composition and Recycling
… an iodinating system into the confines of the phagolysosome, allowing us to study the nature of the phagolysosomal membrane. Lactoperoxidase (LPO) is covalently coupled to carboxylated latex spheres (LPO-latex) in a stable enzymatically active form. The addition of LPO-Iatex to cultured …
-
Investigation of novel therapeutic strategies in B cell and antibody mediated disease
… DC activation by IgG. We show that I-BET delays phagolysosome maturation associated with build-up of immune complex (IC) whilst selectively inhibiting IC induced cytokine production. I-BET changed MNP morphology, resulting in a less adherent phenotype, prompting an assessment of its impact on DC …
-
Sortases: Keystones to Virulence and Targets for Anti-Infective Therapy
… These findings support SrtA activity inside the phagolysosome of a professional phagocyte and likely contribute to the ability of <italic>S. aureus</italic> to evade the innate immune system.</p><p>The roles of sortases and their substrates during <italic>S. aureus</italic> survival inside …
-
Role of Bacterial Effectors SopD and SopB in Pathogenicity of Salmonella enterica serovar Typhimurium.
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that has evolved to take advantage of the eukaryotic host cells it inhabits during infection. It uses bacterial effectors translocated into the host cell cytosol to manipulate host cell machinery and establish a …