Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"phage library"”.
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Studies on the antibody repertoire in a Dengue virus immune subject and isolation of neutralizing antibodies by phage display technology
… of EDIII in DENV neutralization. In addition, Phage Display Technology was utilized to generate a DENV-immune Fab phage library for investigation of antibody repertoire upon infection, and for identification of EDIII-specific Fabs that are highly neutralizing.
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Genetic engineering of bacteriophage and its applications for biomimetic materials
Filamentous bacteriophage (M13) are excellent biological build block due to their multiple peptide display system including type 8 (complete peptide display at pVIII) and type 83 (complete peptide display at both pVIII and pIII) display systems. Unlike the phagemid systems, the advantage of these …
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Development of bispecific filamentous bacteriophages for the generation of a novel automated screening system based on phage display technology
Phage display is a powerful tool for screening large protein libraries in order to identify novel therapeutics, diagnostics or vaccines. In the emerging field of protein based drug development, there is an urgent need for high-throughput technologies for automated library handling and phage display …
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Assembly of biological building blocks for nano- and micro-fabrication of materials
… using genetically engineered M13 bacteriophage. This virus template showed superior controls of material syntheses from nanoscale to microscale. Structures including nanowires, nanoparticle arrays, hetero-particle arrays, and micro-fibers were fabricated using the engineered MI3 virus as …
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Generierung, Charakterisierung und funktionaler Assay von Antikörpern und Antikörperfragmenten gegen die HCV Strukturproteine core, E1 und E2
… project for screening of a human Hepatitis phage-library. Arising from these analysis thirteen recombinant antibodies were identified and their amino acid sequences were further characterised.
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Isolation of Therapeutic Antibodies Against Microseminoprotien to Potentially Overcome Resistance to Anti-Angiogenic Therapy
… of this study was to use the diverse scFv phage library in our laboratory to develop antibody probes. These will help us investigate the extent MSMP has on cancer biology.</p> <p>Our work involved generating a panel of six monoclonal antibodies (mAbs) against MSMP, which shows potential as …
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Identifying B-cell epitopes of African horse sickness virus serotype 4 recognised by antisera of immunised horses
… sera from immunised horses and a genome-targeted phage display library. A phage library expressing a repertoire of AHSV-4 peptides large enough to represent the whole genome was constructed. The library was affinity selected with purified naïve (day 0) and immunised (day 28, day 52) horse IgGs and …
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Isolation And Analysis Of Peptides Binding To Helix 69 Of E. Coli 23 S Rrna From M13 Phage Display
… E.coli were being selected from a heptapeptide phage library. An experimental system including biotin labeling of RNA and then affinity selection through four rounds was being followed. After sequencing phage clones of the fourth round, two peptide sequences dominated the phage pool, STYTSVS and …
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Development of recombinant antibody mediated resistance against Tomato yellow leaf curl virus
… as fusion proteins with GST and MBP. Using phage display technology one scFv fragments against Rep (scFv-ScRep1) was isolated through panning of naïve Tomlinson I scFv phage library. In addition, an ARep phage display library constructed from total spleen RNA of a mouse immunized with …
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The isolation of single chain variable region fragments (scFvs) from a phage display library, and expression of the isolated scFvs in Nicotiana benthamiana
… This dissertation describes the use of phage display technology to screen for and identify scFvs that bind to three different test antigens. Phage display library technology involving the expression and presentation of antibody or antibody derivatives on the coat surfaces of phage …
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Peptide-Based Platform For Cancer Therapeutics And Drug Transportation Across Blood-Brain Barrier
… the BBB. This work will be completed using the phage display technique. In chapter 1 and 2, we introduced and discussed the background of prostate cancer and the utilization of prostate cancer relevant antigens and enzyme for targeted drug delivery. We also discussed the role of the BBB as a …
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Elucidation of the Molecular Mechanisms Underlying Estrogen-Mediated Estrogen Receptor Activation
… by the overall surface topography of the sNR. Phage display is a widely-used research technique for screening highly diverse peptide libraries to enrich for sNR-binding clones. It requires two primary components for affinity selection: (1) a phage display cDNA library, and (2) purified …