Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"phage display technology"”.
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Studies on the antibody repertoire in a Dengue virus immune subject and isolation of neutralizing antibodies by phage display technology
… of EDIII in DENV neutralization. In addition, Phage Display Technology was utilized to generate a DENV-immune Fab phage library for investigation of antibody repertoire upon infection, and for identification of EDIII-specific Fabs that are highly neutralizing.
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Characterisation of STRO-1 expression on human mesenchymal stem cells and identification of putative cancer stem cells in osteosarcoma: prevention by micronutrients
… two techniques were implemented: peptide phage display technology and a microarray based approach. Changes in the expression of STRO-1 were investigated during culture expansion of hMSCs. STRO-1 expression positively correlated with cellular morphology and multilineage potential, whereby …
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Phage Display to Identify Peptides Binding to or Penetrating the Mouse Zona Pellucida
… study was to identify peptide ligands, using phage display techniques, which bind sites on mouse embryos, ovaries, cytoplasmic membranes and/or intracytoplasmic components. Specifically, M13 coliphage 7-mer, 12-mer and 15-mer random peptide libraries were used separately for biopanning. …
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Antibody phage-displayed libraries derived from chicken immunoglobulin genes : a source of highly specific diagnostic antibodies
… (IgY) against four of the five antigens. Using phage display technology, two single-chain variable fragment (scFv) antibody libraries, one with the immunoglobulin VH and VL chain regions joined by a single amino acid (G) and the other with a 15 amino acid flexible linker [(G4S) 3] were …
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Cell and nanomaterial-based approaches for diagnosis and chemotherapy of metastatic cancer cells
… for isolation and detection of CTCs. Using phage display technology, it was possible to identify candidate peptides specific to mesenchymal-like tumor cells that may mimic the motile and aggressive CTC population. In order to detect and ablate metastases and CTCs, M13 bacteriophage was …
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Pretargeted cancer radioimmunotherapy and radioimaging using antibodies and antibody fragments
… target for cancer imaging and therapy. Using phage display technology, TF binding scFv fragments were selected from the McCafferty antibody library. The selected scFv clones were characterized in vitro for their TF specificity and cell binding properties by ELISA and flow-cytometry assay. The …
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Characterization of Cytotoxicity against Cancer Cells induced by Human Monoclonal Antibody Specific to TRAIL-R1
… to DR4 from human antibody library using phage-display technology. We characterized the Fab and tested possibility of its usage as anti-cancer agents. Also, we tested combined effect of DR4-4 Fab and r-irradiation. MATERIALS AND METHODS: We generated DR4-4 Fab using phage-display …
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Engineering and characterisation of anti-progesterone OBodies
… with nanomolar affinity as a proof-of-concept technology and has been given the name OBodies. This thesis explores this concept further with an OBody (D7) engineered to bind to the small molecule progesterone (P4) with potential applications to detect P4 in cow’s milk to evaluate pregnancy. …
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The Development of a New Cloning Strategy for the Biosynthetic Production of Brush-Forming Poly(Amino Acids)
… numerous applications. Through recombinant DNA technology, there exists the potential to harness a cell's protein synthesis machinery to produce a brush-forming poly(amino acid) (or PAA) with an exactly specified amino acid sequence, thus controlling the polymer's composition at a level …
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Development of novel antibody-based diagnostics for the early and rapid detection of cardiac markers
… antibody generation and ‘state-of-the-art’ phage display technology were employed for the generation of CRP- and MPO-specific antibodies and recombinant fragments. The immune systems of mice, rabbits and chickens were investigated in this work, which allowed for the identification of the …
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Molecular Interactions of Human C9: The Pore Forming Protein of the Complement System
… binding interactions. Another approach used phage display technology to further narrow the binding sites on C9 for C8α-γ and C9. Two separate phage display systems were employed for accomplishing this goal: commercially available combinatorial libraries and a custom library containing …
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A Point-of-Care Immunoassay for Ultra-Sensitive Detection of Ebolavirus
… antibodies was achieved by associating scFv phage-display technology with the transient expression of promising scFv candidates as Fc fusions in mammalian cells followed by their seamless purification with an IsoTag based chromatography-free system. These elements when combined with a novel …
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Development of antibodies for characterizing the Arabidopsis flavonoid biosynthetic pathway
… pathway were developed using conventional and phage antibody technology. cDNAs from Arabidopsis coding regions of chalcone synthase (CHS) and chalcone isomerase (CHI) were sub-cloned in frame into a bacterial expression vector as fusions with glutathione Stransferase (GST) using standard …
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The isolation of single chain variable region fragments (scFvs) from a phage display library, and expression of the isolated scFvs in Nicotiana benthamiana
… antibodies have been produced using hybridoma technology. They have also been successfully produced in other expression hosts such as bacteria, yeasts, insect cells and mammalian cell lines. However, these expression systems come with a few disadvantages, some of which include high maintenance …
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A Screen For Peptides Targeting Chemoresistant Metastatic Triple-Negative Breast Cancer
… this hypothesis, we performed a combinatorial phage-displayed peptide library screen to isolate phage-displayed cyclic peptides that mimic the natural ligands of novel receptors expressed by chemoresistant metastatic TNBC tumor cells. These peptides were termed Breast Lung Metastasis Peptides …