Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 133 for “"phage display"”.
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Analyse von Protein-Protein-Wechselwirkungen mittels Phage-Display
… beide Möglichkeiten gefunden. Die über das Phage Display angereicherten Sequenzen konnten in der Aminosäuresequenz des Knob nicht als lineare Abfolge gefunden werden. Unter Zuhilfenahme der Kristallstruktur des Knob wurden Vorschläge für die Lokalisation der Antikörperepitope gemacht. Diese …
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Mapping The Human Vasculature By In Vivo Phage Display
<p><em>In vivo</em> phage display screenings by intravenous injection of a random phage-displayed peptide library allow for the selection of peptides that localize to specific vascular beds. At the University of Texas MD Anderson Cancer Center, we have had the opportunity to perform phage display …
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Exploring Protein -Rna Interactions With Site-Directed Mutagenesis and Phage Display
… RNA recognition is required. We have applied phage display and site-directed mutagenesis to dissect principles of RRM-controlled RNA recognition. The model systems we are investigating are U1A and CUG-BP1. In this dissertation, the molecular basis of the binding affinity of U1A-RNA beyond …
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Exploring Protein-Rna Interactions With Site-Directed Mutagenesis and Phage Display
… RNA recognition is required. We have applied phage display and site-directed mutagenesis to dissect principles of RRM-controlled RNA recognition. The model systems we are investigating are U1A and CUG-BP1. In this dissertation, the molecular basis of the binding affinity of U1A-RNA beyond …
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Exploring protein-RNA interactions with site-directed mutagenesis and phage display
… RNA recognition is required. We have applied phage display and site-directed mutagenesis to dissect principles of RRM-controlled RNA recognition. The model systems we are investigating are U1A and CUG-BP1. In this dissertation, the molecular basis of the binding affinity of U1A-RNA beyond …
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Phage Display Library Screening For Psa-/Lo Prostate Cancer Cell-Binding Peptides
… resistance. My master thesis research employs a phage display library screening technology aiming to identify peptides that preferentially home in to undifferentiated PCa cells, which our lab has previously demonstrated to be intrinsically resistant to castration. <strong></strong></p> <p>There …
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In vitro and in vivo approaches to tumor targeting by phage display
… commonly used in vitro, ex vivo, and in vivo phage display techniques and binding assays in an attempt to discover such peptides. This work lead to the discovery a phage mutant which interferes with the usage of the phage display libraries of the type used during this research. In addition, I …
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A study of peptide affinity for carbon nanotubes through phage display experiments
… and interactions. This was done using a phage display technique, in which a library of bacteriophages displaying greater than 109 different 12-amino acid long peptide sequences were exposed to carbon nanotubes. Non-specifically bound phages were successively washed off with increasingly …
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Phage Display to Identify Peptides Binding to or Penetrating the Mouse Zona Pellucida
… study was to identify peptide ligands, using phage display techniques, which bind sites on mouse embryos, ovaries, cytoplasmic membranes and/or intracytoplasmic components. Specifically, M13 coliphage 7-mer, 12-mer and 15-mer random peptide libraries were used separately for biopanning. …
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Identifikation und Charakterisierung neuer immunogener Proteine und anschließende Generierung rekombinanter Antikörper mittels Phage Display
… Arbeit vier verschiedene Humanpathogene mittels Phage Display untersucht: Neisseria gonorrhoeae, Neisseria meningitidis, Borrelia burgdorferi und Clostridium difficile. Hierfür wurden aus der genomischen DNA der vier Erreger Bibliotheken konstruiert und durch wiederholte Selektion und …
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Mapping the Major Epitope(s) of the Glycoprotein of Ebolavirus with QB Phage Display System
… of the research was to produce and analyze Qβ phage virions containing various segments of the EBOV glycoprotein fused to its A1 the readthrough minor coat protein. The recombinant phages would then be utilized to analyze their antigenicity and to map the major epitope(s) of the glycoprotein …
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Isolation And Analysis Of Peptides Binding To Helix 69 Of E. Coli 23 S Rrna From M13 Phage Display
… E.coli were being selected from a heptapeptide phage library. An experimental system including biotin labeling of RNA and then affinity selection through four rounds was being followed. After sequencing phage clones of the fourth round, two peptide sequences dominated the phage pool, STYTSVS and …
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Studies on the antibody repertoire in a Dengue virus immune subject and isolation of neutralizing antibodies by phage display technology
… of EDIII in DENV neutralization. In addition, Phage Display Technology was utilized to generate a DENV-immune Fab phage library for investigation of antibody repertoire upon infection, and for identification of EDIII-specific Fabs that are highly neutralizing.
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The isolation of single chain variable region fragments (scFvs) from a phage display library, and expression of the isolated scFvs in Nicotiana benthamiana
… This dissertation describes the use of phage display technology to screen for and identify scFvs that bind to three different test antigens. Phage display library technology involving the expression and presentation of antibody or antibody derivatives on the coat surfaces of phage …
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Development of a Dusky kob scFv gene phage display library for the discovery of antibodies to Brome mosaic virus - a proxy for a novel, emerging fish pathogen
… opportunistic pathogens and parasites. Antibody phage display is a method that allows foreign peptides or proteins to be expressed on the phage surface through translational fusion with phage coat proteins. Consequently, antibodies expressed by a diverse repertoire of genes coding for the single …
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Expression of Foreign Genes in the Pseudomonas Bacteriophage Pf3
Filamentous bacteriophages were engineered to express foreign genes with the ultimate purpose of displaying transmission control anti-malarial peptides as in phage display. It was hypothesized that expression of foreign genes would be possible using the phage’s promoters. This hypothesis was tested …
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Genetic engineering of recombinant anti-mycolic acid antibody fragments for use in tuberculosis diagnostics
… walls of Mycobacterium tuberculosis. The Nkuku phage display library was previously used to obtain monoclonal antibody binders to mycolic acids. In total 11 binders were obtained of which one was selected (MAC10) for further investigation by genetic engineering as presented in this dissertation. …
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