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Showing 1 to 15 of 15 for “"ph profile"”.
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Spectrophotometric and microbiological determination of the stability of sodium ampicillin as affected by pH
… investigation was to determine the effect of pH on the stability of ampicillin sodium for injection. Then, to correlate this information, a pH profile may be established to predict chemical stability with ampicillin sodium alone or when other drugs may be included in the admixture. With a pH …
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The Mechanism of Activation of Potassium Dependent and Potassium Independent Asparaginases from Common Bean (Phaseolus vulgaris)
… This study investigated the biochemical and biophysical properties of potassium dependent (PvAspG1) and potassium independent (PvAspG-T2) L-asparaginases from P. vulgaris. Previous studies revealed that PvAspG1 requires potassium for catalytic activation and crystal structure analysis suggested …
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Flavin Amine Oxidases from the Monoamine Oxidase Structural Family Utilize a Hydride Transfer Mechanism
… the oxidation of N1-acetylated polyamines. The pH-dependence of the kcat/Kamine indicates that the monoprotonated form of the substrate is required for catalysis, with the N4 nitrogen next to the site of CH bond cleavage being unprotonated. Stopped-flow spectroscopy shows that the pH-dependence …
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Kinetic Analysis and pH Dependence of the Phosphite Dehydrogenase Reaction
The pH-rate profiles for active site mutants Lys76Ala, Glu266G1n, and Arg237Lys are also bell-shaped and the higher pKa of each is 8.4, which is also consistent with that observed for the pH dependence of sulfite inhibition for the wild type enzyme. Interestingly, the acidic limb of the Glu266Gln …
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Stability Study of Phoslactomycin B and Analysis of Degradation Products
Phoslactomycin B (PLM-B), a potent and selective inhibitor of serine threonine phosphatase is of interest for its antitumor, antifungal and antiviral activity. The objective of this study was to evaluate the stability of phoslactomycin B at various pH and temperature conditions. Phoslactomycin B …
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Developing an optical sensor for the visualisation of pH gradients in marine sediments
… in marine sediments are intimately linked to pH dependent processes, but surprisingly little is known about the details of the pH profiles generated. This is mainly due to the difficulty of obtaining samples and measuring representative profiles. Such profiles have previously been determined …
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Mechanistic studies on chorismate mutase-prephenate dehydrogenase from E. coli
Chorismate mutase-prephenate dehydrogenase is a bifunctional enzyme that catalyzes two sequential reactions in the biosynthesis of tyrosine in E. coli and other enteric bacteria. Chorismate mutase catalyzes the pericyclic rearrangement of chorismate to prephenate which is subsequently converted to …
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The design and installation of an automatically controlled hydrocarbon fractionation unit
… venom was purified over 1000-fold to electrophoretic homogeneity through a 3-step procedure which included affinity chromatography on Cibacron Blue agarose. The enzyme exhibited a broad pH profile with the optimum range between 7-8. Studies on the substrate specificity of B. fasciatus venom …
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Purification and properties of Bungarus fasciatus venom NAD glycohydrolase
… venom was purified over 1000-fold to electrophoretic homogeneity through a 3-step procedure which included affinity chromatography on Cibacron Blue agarose. The enzyme exhibited a broad pH profile with the optimum range between 7-8. Studies on the substrate specificity of B. fasciatus venom …
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Duodenal pH : new aspects of physiology and pathophysiology
… Previous investigations of the duodenal pH have been restricted by methodological and technical difficulties, and have, for the most part, only monitored the pH in the short-term. A new reliable system for long-term (twenty-four hour), ambulatory, simultaneous measurement of intra-luminal …
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Fundamental phenomenological description and experimental optimization of gel stabilized biocatalysts in a two-phase system
… consequence of the acidification of the aqueous phase lead to the evolution of the calcium-alginate network to an alginic acid one with a size reduction of the spheres and a release of 10-15 % of water (w/w) according to the calcium-alginate preparation. The control of the pH can be only achieved …
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Development and Application of a Classification System for Undisturbed and Disturbed Tropical Montane Forests Based on Vegetation Structure
… Primary ravine forest at higher altitude, Microphyll ridge forest, Mesophyll ridge forest, Macrophyll ridge forest, and megaphyll ridge forest. Two forest types were investigated outside the closer investigation area and were classified as Secondary forest in Mindo and Primary forest in …
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Mechanistic Modeling, Design, and Optimization of Low Salinity Waterflooding
… specially designed for the modeling of LSW physical phenomena, has been developed and successfully implemented in Computer Modelling Group’s GEM™ reservoir simulator. Not limited to pure LSW, it can also be used to model and design hybrid EOR LSW techniques such as Low Salinity …
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STRUCTURE-FUNCTION STUDIES OF NOVEL MEDICALLY RELEVANT FLAVOENZYMES
… of an amino acid, or activation of dioxygen, to photochemistry; from DNA damage repair to light emission. Recently, genomics trascriptomic and proteomic approaches led to the identification of new flavoproteins playing a key role in the metabolism of many organisms. Several of these new enzymes …
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Metagenomanalyse eines hydrolytischen Konsortiums: Identifizierung und biochemische Charakterisierung von Polysaccharid-abbauenden Biokatalysatoren aus nicht kultivierten Mikroorganismen
Die Durchmusterung von Umweltgenbanken hat sich in den letzten Jahren als effektive Technik erwiesen, auf den stetig wachsenden Bedarf der Industrie an neuen Biokatalysatoren zu reagieren. Im Rahmen dieser Arbeit wurde eine Umwelt-Genbank eines Boden-Konsortiums durch die Kombination …