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Showing 1 to 20 of 34 for “"peroxiredoxin"”.
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Studies in Peroxiredoxin Hyperoxidation Sensitivity
… focuses on the structure and biochemistry of peroxiredoxins (Prx) and their sensitivity to inactivation by their own substrate, in a process called hyperoxidation. Hyperoxidation sensitivity is important to study because Prxs are implicated in many different diseases. For example, Prxs …
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CLASSIFYING PEROXIREDOXIN SUBGROUPS AND IDENTIFYING DISCRIMINATING MOTIFS VIA MACHINE LEARNING
… that our 3mer-SVM, that accurately classifies Peroxiredoxin subgroups, can provide meaningful additional insight into the functional conserved sites in Peroxiredoxin protein. Moreover, in Chapter 4, we propose a two-round learning algorithm that can capture gapped-kmer features in sequences and …
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Expression of Peroxiredoxin Induced by Silica in Alveola Epithelial Cells
… 동시에 공존한다고 할 수 있다. 비교적 최근에 알려진, 새로운 계통의 항산화 효소인 peroxiredoxin (Prx)은 세포의 증식과 분화, 세포사멸이나 발암과정에 관여하는 것으로 알려져 있다. 더구나, 이들 Prx 는 thioredoxin (Trx)과 더불어 열충격이나 산화스트레스에 의존적인 분자 생물학적 스위치로서, peroxidase 와 chaperone 기능과의 사이를 조절하는 것으로 알려져 있다. 하지만, 현재까지 폐암을 비롯한 각종 질병에서의 이들 Prx의 역할은 잘 규명되어 있지 않으며, 분해 및 대사과정에 …
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Study on the Physical and Functional Interaction between Fortilin and Peroxiredoxin I
… proteins, including the anti-oxidant enzyme peroxiredoxin I (Prx I). Prx I is a thioredoxin-dependent peroxidase enzyme that is known to be protective against reactive oxygen species (ROS)-induced cell death. We confirmed the interaction between FT and Prx I by using various approaches, …
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KINETIC, THERMODYNAMIC AND MECHANISTIC FEATURES OF ESCHERICHIA COLI BCP, AN UNUSUALLY VERSATILE PEROXIREDOXIN
… (<italic>Ec</italic>BCP) by definition is a peroxiredoxin (Prx) which catalyzes the reduction of hydrogen peroxide and organic hydroperoxides. <italic>Ec</italic>BCP is a member of the most poorly characterized and least studied Prx subfamily. The experiments and data presented in this thesis …
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Characterisation of XvPrx2 : a type II peroxiredoxin isolated from the resurrection plant Xerophyta viscosa (Baker)
… revealed the identity of sixty genes. A type 2 peroxiredoxin (XvPrx2) was selected for further analyses as it was observed, by northern analyses, to be stress-inducible. The XvPrx2 protein was confirmed to be involved in the stress response by Western analyses. The XvPrx2 gene, which displays …
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Design and application of a genetically-encoded probe for peroxiredoxin-2 oxidation in human cells
Hydrogen peroxide (H₂O₂) is a well-known oxidant species commonly produced in eukaryotic organisms as a result of cellular metabolism that plays a central role in numerous processes in cells, and dysregulation of this species can result in a number of different disease states in human cells. In the …
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Nrf2-Dependent Overexpression of Sulfiredoxin and Peroxiredoxin III in Human Lung Cancer, Especially Squamous Cell Carcinoma
"TABLE OF CONTENTS ABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES v ABBREVIATION vi Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 6 A. Materials 6 B. Subjects 6 C. Cell culture and viability 7 D. Transfection and depletion of siRNA 7 E. Immunocytochemistry 8 F. Co-immunoprecipitation and immunoblot …
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Oxidativer Stress- assoziierter neuronaler Zelltod und die Identifikation neuroprotektiver Gene durch ein neuartiges Screening-System
… Vier der sechs Gene: Glutathion Peroxidase-1, Peroxiredoxin-1, Peroxiredoxin-5 und Katalase, kodieren direkt antioxidativ wirkende Genprodukte, deren Identifikation die Funktionalität des Screening-Systems bestätigte. Neben Genen, deren Proteintranskripte direkt antioxidativ wirken, konnte des …
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Identifizierung infektionsrelevanter Antigene des Schimmelpilzes Aspergillus fumigatus sowie deren rekombinante Herstellung mit dem Ziel der Entwicklung eines Impfstoffes gegen die invasive Aspergillose
… Antigen-Kandidaten (ADAM-A, ADAM-B, Aspf3(Peroxiredoxin)) Gen-Deletionsmutanten hergestellt und biochemisch charakterisiert. Zu der Mutante delAspf3 findet sich ein deutlicher Phänotyp, wenn dem Medium Wasserstoffperoxid zugefügt wird. Dieser Phänotyp äußert sich durch eingeschränktes oder …
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The Role of Peroxiredixin Ⅲ in the ototoxic drug-induced mitochondrial apoptosis of cochlear hair cell
… mostly by catalase, glutathione peroxidase, and peroxiredoxin(Prx). The newly identified Prx family of peroxidases includes at least six isoforms in mammalian cells. Among them, Prx III is synthesized with a mitochondrial targeting sequence and is then transferred to mitochondria. We have shown …
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PROTEOMICS INSIGHTS INTO MOLECULAR MECHANISMS OF TRIPTOLIDE AGAINST COLORECTAL CANCER
… Profiling (ABPP) and proteomic approaches. Peroxiredoxin I and Annexin A1 were validated to be the direct binding targets of triptolide. Proteomics approaches allows the study of altered proteins by triptolide and binding targets of triptolide at a global level, which provides some insight …
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Human telomerase reverse transcriptase (hTERT) overexpression modulates intracellular redox balance and protects cancer cells from apoptotic cell death
… as well as efficient recovery of the oxidized peroxiredoxin to its non-oxidized form. Our data also provide evidence for mitochondrial localization of hTERT, and a significantly higher activity of cytochrome c oxidase, the rate-limiting enzyme in the mitochondrial electron transport chain, in …
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Applications of Engineered Proteins in Redox Biology and Biomarker Detection Assay Development
… that the primary respondent of cytosolic H₂O₂ is peroxiredoxin 1 and the H₂O₂ induced apoptosis initiates before the collapse of Prx/Trx/TR antioxidant network. Then, a genetically encoded FRET sensor was used to design a high-throughput screening assay that identified three small-molecule drugs …
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Antioxidant PRDX1 limits CD8+ T cell effector function in the tumour microenvironment
… surprisingly, has profound antioxidant activity. Peroxiredoxin 1 (PRDX1) was identified as the most differentially enriched antioxidant protein in TIF. Individual environmental supplementation of PRDX1 was sufficient to exert both an antioxidant and suppressive effect on CD8+ T cell effector …
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Disulfide Bond Formation: Identifying Roles of PDI Family Thiol Oxidoreductases and ER Oxidant Pathways
… Depletion of two other potential ER oxidases, peroxiredoxin 4 (PRDX4) and Vitamin K epoxide reductase (VKOR), showed more modest effects. Upon co-depletion of Ero1 and other oxidases, additive effects were observed, culminating in cell death following combined removal of Ero1, PRDX4, and VKOR …
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Adiporedoxin, an upstream modulator of endoplasmic reticulum oxidative folding and protein secretion
… endoplasmic reticulum oxidoreductase 1, and peroxiredoxin 4. Adrx is oxidized within the ER after treatment with hydrogen peroxide (H2O2) and can reduce H2O2 in vitro, suggesting it also acts as an antioxidant. The overexpression of Adrx in adipocytes protected the ER from oxidative stress …
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Reactive Oxygen Species-Induced Necrotic Cell Death
… Moreover, TGHQ caused a sequential oxidation of peroxiredoxin III (PrxIII), a protein considered the primary antioxidant defense within mitochondria. Thus, TGHQ induced two acidic shifts in PrxIII, with both pI shifted spots representing oxidized forms of PrxIII. Transient expression of a …
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Proteomic investigation of the molecular targets of mycophenolic acid in human cells
… subunit beta, cytochrome b-c1 complex subunit, peroxiredoxin 1, thioredoxin domain-containing protein 12, myosin regulatory light chain 2, and profilin 1), while 5 proteins were down-regulated (protein SET, stathmin, 40S ribosomal protein S12, histone H2B type 1 A, and histone H2B type …
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Oxidative Stress Based Strategies For Enhancing The Efficacy of Histone Deacetylase Inhibitors (Hdaci)
… oxidase 1, glutathione peroxidase-5, -6, peroxiredoxin 6 and myeloperoxidase. Taken together, these experimental results indicate that the synergistic activity of two different HDACi with adaphostin is mediated by distinct redox mechanisms in ALL cells. Understanding the mechanism involved …
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