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Showing 1 to 20 of 53 for “"peroxidases"”.
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Evolutionary and Paleobiochemical Analyses of Heme Peroxidases
… The approach presented here, applied to the heme peroxidases, marries bioinformatic methods with evolutionary theory and biochemical validations. Heme peroxidases catalyse the oxidation of a variety of electron donors by hydrogen peroxide. These enzymes can be classified into two major families …
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Transcriptomics-Guided Biodiscovery of Peroxidases from Macroalgae
Peroxidases are versatile enzymes with applications in laboratory analytics and industrial processes. Despite their relevance, many applications rely on a few well-characterized terrestrial enzymes, particularly horseradish peroxidase (HRP), whose complex post-translational modifications hinder …
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Potential Roles of Peroxidases In Caenorhabditis Elegans Innate Immunity
… characterized a group of previously unstudied peroxidases in <em>C. elegans </em>and determined their involvement in the host immune response to <em>Enterococcus faecalis</em>. In particular, I focused on SKPO-1 (ShkT-containing peroxidase) and how it contributes to the host immune response …
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Isolating, characterizing, and engineering novel Cu-proteins and peroxidases
Metalloproteins are a fascinating class of proteins that function at the heart of several important biological processes including photosynthesis, respiration, and nitrogen fixation. It is even more amazing, considering that nature uses a small set of tertiary structures and metal centers to …
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Green Pea Peroxidases: Their Catalytic Properties and Thermal Stabilities
A simple and effective method of identifying multiple forms of peroxidase isoenzymes was developed using analytical thin-layer isoelectric focusing (IEF) on polyacrylamide gel (PAG) plates. A total of 18 (pI's 3.5-9.1) peroxidase isoenzymes were identified from crude extracts of pea seeds; all
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Engineered heme peroxidases as genetically encoded probes with diverse applications in cell biology
Proteins perform myriad functions inside cells. In vitro studies of purified proteins yield important information about their function, but it is an important pursuit in cell biology to study proteins within the native context of intact cells, since their physiological functions might not be …
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Inhibition of horseradish peroxidase activity by specific antibody
… in the presence of antibody. In addition, acidic peroxidases that are less susceptible to peroxide are poorly inhibited by antibasic horseradish peroxidase. Spectrophotometric experiments with an electron donor analogue indicated that antibody does not inhibit horseradish peroxidase activity by …
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Host preference of Perkinsus species: epizootiological, environmental, and molecular aspects
… enzymes include superoxide dismutases (SODs) and peroxidases such as catalases. SODs have been characterized in P. marinus, but peroxidases have not been detected. Results from the present study suggest that, while lacking catalase, P. marinus has ascorbate dependent peroxidases usually found in …
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Analysis of the peroxidase-catalysed oxidation of hydroxamic acids
… insight into structure-function relationships of peroxidases. Hydroxamic acids acted as inhibitors of the native state (E), by formation of an E-hydroxamic acid complex that prevents H2O2 from binding. Hydroxamic acids donate one proton and one electron to peroxidase intermediates (CI and CII) and …
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Biomimetic emulators of high potential peroxygenases: Implications in bioremediation and metabolic studies
… metalloporphines can emulate the active site of peroxidases and peroxygenases, their use in several bioremediation processes has been analyzed in this work. The described catalytic reactions use bioinspired, homogenized or heterogenized, commercial porphines and showed a remarkable ability to …
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Characterization of the Active Site of Chloroperoxidase Using Physical Techniques (Hemeproteins)
… of the structure-function relationships between peroxidases, catalase and cytochrome P-450 hemeproteins.
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Mechanistic study and protein engineering of a dye-decolorizing peroxidase from Thermomonospora curvata for enhanced and expanded functions
… specificity, and stability. Dye-decolorizing peroxidases (DyPs), a versatile class of heme enzymes, are particularly attractive for industrial and environmental applications, including lignin valorization and dye degradation. However, their catalytic potential is often limited by intrinsic …
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Peroxidação de lipídios em membranas e tecidos de dois cultivares de sorgo (Sorghum bicolor (L.) Moench) com tolerância diferencial ao alumínio
… alterada na presença do Al. A atividade das peroxidases aumentou nas raízes e nas folhas dos dois cultivares, principalmente no sensível. De modo contrário, a atividade das peroxidases do ascorbato e das catalases reduziu nesses tecidos, exceto nas raízes do cultivar sensível, onde o aumento …
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Meccanismo catalitico della perossidasi da Euphorbia characias
Peroxidases (EC 1.11.1.1-16) are a group of oxidoreductases utilizing hydrogen peroxide, or other peroxides, to oxidize a second reducing substrate belonging to a large array of organic and inorganic compounds. A class III peroxidase isolated from the latex of Mediterranean shrub Euphorbia …
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Multinuclear NMR Studies of Hemoproteins and Their Model Compounds
… Oxygen-17 NMR studies of various carbonmonoxy peroxidases demonstrated that the peroxidases exist in two distinct states, which undergo reversible acid-base induced transitions characterized by a single pK value. Finally, the nature of the iron-oxygen bond in the heme model compound, $\sp …
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SPECTROSCOPIC CHARACTERIZATIONS OF THE COMPOUND II INTERMEDIATE OF SOYBEAN PEROXIDASE FROM SOYBEAN SEED COATINGS
… vibrational stretching frequencies of the ferryl peroxidases varied with varying pH/pD were observed within the 773–787cm-1 range. From the deuterium experiment, accompanied with changes in the vibrational frequencies of the iron-ligand, a 3cm-1 upshift and intense resonant enhancement of the …
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Heme Peroxidase Hpx-2 Protects Caenorhabditis Elegans From Pathogens
<p>Heme-containing peroxidases are important components of innate immunity. Many of them functionally associate with NADPH oxidase (NOX)/dual oxidase (DUOX) enzymes by using the hydrogen peroxide they generate in downstream reactions. In <em>Caenorhabditis elegans,</em> a ShkT-containing …
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Biochemical Studies on Enzymatic Treatment of Pulp and Paper Mill Biosludge and Polymeric Lignin
… bacterial multicopper oxidases (MCOs) and heme-peroxidases and identified oxidase activity against polymeric lignin in 22 proteins. Among them, the Bacillus subtilis laccase BSU0630 (CotA) demonstrated the highest activity towards both soluble substrates and polymeric lignin. Structure-based …
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Isozyme patterns of selected isolates of Pholiota in the stirps "Adiposa"
… phenoloxidases (laccases and tyrosinases), and peroxidases on polyacrylamide gels. Harvesting of different isolates for protein extraction was carried out at comparable stages of development using dry weight determinations and respiration studies as criteria. Peroxidase and phenoloxidase …
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