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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"periplasmic space"”.
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Studies on the mode of action of diacetyl and carbon dioxide as inhibitors of meat spoilage bacteria
… of arginine. This inhibition occurs in the periplasmic space and CO2 may exert its influence in the periplasmic space as well. Therefore, this study investigated the effect of CO2 and diacetyl alone or combined on the Gram-negative spoilage organisms found in ground beef. The effect of …
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Leucyl-tRNA synthetase: dynamic subcellular relocalization and drug resistance mechanism
… were identified outside the cytoplasm, in the periplasmic space and also associated with the membrane. Under certain stresses, LeuRS and tRNALeu re-located into the media. In some cases, LeuRS appeared to be fragmented with one part associated with the membrane, and a second part in the …
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Development of SNAP-tag-based fusion proteins targeting HIV-1 viral reservoirs
… in the presence of compatible solutes in the periplasmic space of E. coli. SNAP-tag is a self-labelling protein that covalently binds benzylguanine (BG)-modified substrates in a 1:1 stoichiometric ratio. When recombinantly fused to any protein of interest, SNAP-tag allows the stable labelling …
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Comparative biochemistry and genetic analysis of nucleoside hydrolase in Escherichia coli, Pseudomonas aeruginosa, and Pseudomonas fluorescens.
… of 30-35 amino acids that is shown to act as a periplasmic-signaling sequence. These are the first two nucleoside hydrolases, to date,that have been conclusively demonstrated to be exported to the periplasmic space. The physiological relevance of this is explained.
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Cloning, High-Level Expression, and Characterization of Escherichia Coli Cytochrome B(562)
… cytochrome b$\sb{562}$ is translocated to the periplasmic space. Similar experiments on cytochrome b$\sb{562}$-M7L (a point mutant of cytochrome b$\sb{562}$ in which one of the heme axial ligands, methionine, was replaced by leucine), that was created in a site directed manner, revealed that …
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The regulation and two genes in Bacteroides thetaiotaomicron starch utilization system
… to its surface, then translocating it into the periplasmic space. Several genes have been identified that encode enzymes or outer membrane proteins involved in starch utilization. These have been called sus genes, for starch utilization system. Previous studies have shown that sus structural …
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Tales of Seduction and Betrayal: Host Genes Required for Assault of Salmonella by Bacteriophage P22
… of P22, preventing the ejection of DNA into the periplasmic space of the recipient bacterium. The mutant also showed pleiotropic defects in outer membrane integrity and growth at 42$\sp\circ$C. (3) DNA uptake. A Ts mutation, tdx, reduced P22-mediated transduction of the recipient by a factor of …
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Expression of Recombinant Chloroperoxidase
… The expressed apoenzyme was secreted into periplasmic space at an expression level representing about 2% of the total cellular protein. The apochloroperoxidase in the insoluble inclusion bodies was solubilized in 8 M urea and partially reconstituted with iron protoporphyrin IX, then the …
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Identification of a novel HIV-1C protease from a microbial source
… thus preventing its translocation to the periplasmic space. This would result in the host cells' sensitivity to β-lactam antibiotics supplemented in the growth media. The presence of an inhibitor would restore resistance and therefore ‘life'. Despite validation of the E. coliscreening …
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Expression and Localization of Green Fluorescent Protein in B. abortus strain RB51
… in B. abortus strain RB51, outer-membrane (OM), periplasmic space (PS) and in the cytoplasmic region (CR) of B. abortus strain RB51. This localization was obtained by transforming strain RB51 with plasmids pBBg18sGFP and pBBgSsGFP, in which the 18 kDa Brucella lipoprotein and the Brucella Cu/Zn …
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Identification and molecular characterization of a putative heme ABC transporter in Neisseria meningitidis
… been elucidated. Once heme is deposited in the periplasmic space, a heme dedicated ABC transporter has been hypothesized to convey heme into the cytoplasm. To address this hypothesis, we used several molecular techniques to identify and characterize a putative heme ABC transporter in Neisseria …
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Molecular and Biochemical Analysis of the Histidine Kinase CusS and its Role in Metal Resistance in Escherichia coli
… to elevated levels of copper or silver in the periplasmic space of E. coli. Extracellular signals are transduced into the cell through phosphotransfer reactions between the prototypical histidine kinase CusS and the response regulator CusR. Copper sensing by the periplasmic domain of CusS is …
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Identification and characterization of the acid phosphatase from Francisella tularensis and its potential role in pathogenesis
… phosphatases, FtACP has been localized to the periplasmic space of the organism. FtACP has the following properties: molecular mass $\sim$56 kDa, pH optimum of 6.0 for five different substrates, isoelectric point of 9.2, broad substrate specificity including. The enzyme was inhibited by …
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Antimicrobial Effectors Act Cooperatively to Stress Salmonella in the Macrophage Phagosome
… macrophages. The bacterium produces two Cu-Zn periplasmic superoxide dismutases, SodCI and SodCII. Although both enzymes are expressed during infection, only SodCI contributes to virulence in the mouse by combating phagocytic superoxide. We have shown that the differential contribution to …
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Improvement of engineered thermostable xylanase production via immobilization of recombinant escherichia coli onto graphene oxide
… of the expressed recombinant enzyme in the periplasmic space. Thus, the utilization of the immobilization in targeting the recombinant enzyme expression in the culture medium, presents substantial preferences over cytoplasmic excretion. Immobilization process can be applied to optimize the …
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Molecular analysis of two cellulase genes from Ruminococcus flavefaciens FD-1 and their transcriptional regulation
… kDa and was secreted into the Escherichia coli periplasmic space. Although CelA has activity on carboxymethylcellulose, further study on the enzyme showed that it degraded cellopentaose and other cellodextrins to predominantly cellobiose. Thus CelA is a cellodextrinase. It also has high activity …
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Development of Brucella abortus RB51 as a Vaccine to Protect Against Brucellosis and Anthrax
… localize the PA4 to the outside cell envelope, periplasmic space or cytosol respectively. Comparisons of the expression level and stability of the native and synthetic PA4 in B. abortus strain RB51 were assessed by immunoblot. The protective efficacy of PA4 expressed in Brucella was assessed by …
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Improving the recovery of "difficult to release" periplasmically-expressed products from recombinant E. Coli
… purification demands. Despite these merits, periplasmic expression systems have not yet fulfilled their true potential, largely due to the lack of reliable general methods for efficient selective release of periplasmically expressed proteins at large-scale. The classical osmotic shock …
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Ionic Control of Porin Permeability
… an optically activated proton pump to increase periplasmic H+ and demonstrate that this reduced porin permeability (monitored by live single-cell imaging). In support of a model dependent on changes in periplasmic ions, molecular dynamic simulations suggest that the pore diameter of porins is …
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Elucidating the bio-electrochemical mechanism of exoelectrogenesis of the cyanobacterium Synechocystis sp. PCC6803
… appendages or layers of the cell boundary. The periplasmic space or outer membrane was identified as significantly contributing to the complex photocurrent profile of cells, ‘gating’ exoelectrogenesis. The complexity was also unravelled with the identification of a putative competing charge …
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