Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"perfusion culture"”.
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Microfluidic perfusion culture for controlling the stem cell microenvironment
… signaling. Especially in conventional static culture, the content of the cell culture media changes constantly over time as cells interact with and modify their surroundings. This thesis explores the use of microfluidic perfusion as a tool for modulating diffusible cell-cell signaling in mESC …
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Characterization of the down regulation of antibody production in high cell density perfusion culture
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Applied Biological Sciences, 1994.
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Novel micro/nanofluidic system for separation and monitoring of cells and proteins in perfusion
… describes the novel cell retention device for perfusion culture based on inertial sorting.
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Continuous Antibody Titer Assessment of the Biomanufacturing Process using Nanofluidic Binding Assays
… are presently utilized to maintain long-term perfusion culture. However, this increases the risk of introducing external contaminants into the cell culture and the analyzed analytes. Sensors used in in-line probe-based approaches must withstand harsh clean-in-place and sterilization-in-place …
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The Biological Impact of High Throughput Continuous Bioprocessing
Micro scale, high throughput perfusion technology is under-developed and not widely utilised in academic or industrial settings for the development of CHO based mAb bioprocesses. Work within this thesis highlights the design and characterisation of a sedimentation method of cell retention and its …
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Recycling and Regeneration of Spent Perfusion Media via Ion Concentration Polarization
… the economic and environmental impact of cell culture media in perfusion mammalian cell culture. We demonstrate a microfluidic separation device engineered to selectively remove metabolic waste products—specifically ammonia and lactate—from spent media using ion concentration polarization. …
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Modulation of Chondrogenic and Osteogenic Differentiation of Mesenchymal Stem Cells through Signals in the Extracellular Microenvironment
… and thus was explored via direct contact co-culture. HSPCs in a low dose with sustained osteogenic induction by dexamethasone accelerated osteogenesis and enhanced mineral deposition, whereas the lack of induction signals affected the spatial distribution of cell populations and minerals. …
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Application of Fluid Flow for Functional Tissue Engineering of Bone Marrow Stromal Cells
… by osteoprogenitor cells in vitro when cultured under the appropriate conditions. Previous work has demonstrated that perfusion culture of osteoprogenitor cells within 3D scaffolds stimulates phenotypic markers of osteoblastic differentiation, but those studies did not determine whether …
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The Use of Dynamic Fluid Flow Strategies for Bone Tissue Engineering Applications
… be beneficial in understanding and developing 3D perfusion culture systems for the creation of a clinically effective engineering bone tissue.
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Analyse verschiedener Netzhautkulturmodelle an der Netzhaut vom adulten Hausschwein
Retina tissue culture represents an in vitro system to analyze neuronal as well as glial interactions and molecular processes. In the present work different retina tissue culture systems from the adult porcine eyes were examined by immunohistology and results compared to freshly isolated retina as …