Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"perfringolysin O (PFO)"”.
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Investigation of the role of the toxins perfringolysin O (PFO) and sialidase in Clostridium perfringens gas gangrene infections
… within macrophages. To examine if the toxin perfringolysin O (PFO) could mediate this, less active variants of PFO were used to examine what occurs between phagosomal escape and cell lysis. The mutant forms of PFO did mediate phagosomal escape in macrophages and were found within macrophages …
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The development of novel HIV-1 vaccines using modified recombinant BCG
… of the detoxified Clostridium perfringens toxin, perfringolysin O (pfo), into the rBCG genome. Upon expression, pfo forms pores in the endosome which facilitates translocation of vaccine derived antigen into the cytoplasm. This modification has been shown to lead to cross priming of T cells and …
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The Interactions of Clostridium Perfringens With Phagocytic Cells
… different toxins. The alpha toxin (PLC) and perfringolysin O (PFO) are thought to be important virulence factors in gangrene. We wished to understand how C. perfringens is capable of avoiding killing by the host immune system, and determine if PLC and PFO play a role in this avoidance. We …
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Characterization Of Streptolysin O As The Translocator For The Cytolysin-Mediated Translocation Process In Streptococcus Pyogenes
… Domain 4 from the related pore-forming protein, perfringolysin O: PFO), is insufficient for CMT. Moreover, CMT is unaffected by extraction of cholesterol to levels that block membrane binding by the cholesterol-dependent PFO protein. Although cholesterol is unnecessary, mutations that interrupt …
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Reversibly neutralized perfringolysin O for intracellular delivery of macromolecules/
… in vitro using protein and siRNA payloads. Perfringolysin O (PFO) is a member of the cholesterol-dependent cytolysin (CDC) family of bacterial toxins whose pores, reaching up to 30nm in diameter, allow the passage of large molecules without a specialized transport mechanism. However, the …