Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"peptidomics"”.
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Proteomics and peptidomics for the study of neurological and metabolic disorders
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-05-01
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Proteomics and peptidomics for investigating metabolic and neurological disorders via liquid chromatography-mass spectrometry
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-08-01
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Functional Peptidomics: Combining Discovery-Based Mass Spectrometry and Neurophysiology to Explore Communication of Phase -Resetting Cues in the Rat Suprachiasmatic Nucleus
This thesis justifies functional peptidomics as a useful approach to understanding intercellular signaling mechanisms regulating SCN clock phase. Directly detecting dynamic release properties of peptides from the SCN brain slice provides new insights on a complex phenomenon phenomenon. The …
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Characterizing cerebellin-short, a novel circadian peptide, in the rat suprachiasmatic nucleus
… located in the hypothalamus. Mass spectrometry peptidomics of the SCN identified the small peptide cerebellin-short (SGSAKBSAIRSTN) consisting of 15 amino acids, which is released from the SCN in circadian fashion. Cerebellin-short is the C-terminus truncated form of the 16 amino acid cerebellin …
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Analysis of enzymatic L/D-peptide isomerization in animals
… not easily detected in mass spectrometry-based peptidomics experiments due to the lack of an associated mass shift. To address this challenge, we have developed a method for the non-targeted detection of DAACPs in biological samples and used it to explore the prevalence of DAACPs in the …
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The metabolic sequelae of oesophago-gastric resection
… RNAseq and mass spectrometry based peptidomics of human and murine enteroendocrine cells in the pre- and post-operative populations revealed no significant change in the underlying cellular pathways for nutrient sensing and gut hormone secretion, indicating that the altered hormone …
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Systems based approaches to unravelling neuropeptide-receptor interactions in nematodes
… tools and available transcriptomic and peptidomics data in the nematode parasite Ascaris suum were used to investigate neuropeptide signalling in the reproductive system of this species, revealing putative conservation of neuropeptide function between the model nematode Caenorhabditis …
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Analyzing peptide release using mass spectrometry
… It is of great interest in the field of peptidomics to focus on functional characterization of proteins products such as cell-to-cell signaling peptides that are released from specific neuronal tissues, whether a brain region or specific cell. A variety of analytical techniques have …
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Liquid chromatography-mass spectrometry-based neuropeptide characterizations across neuronal models
… and analyzes the complement of peptides (termed peptidomics), with sample sizes ranging from whole animals to single organs or tissues. Another is a targeted approach, which focuses on specific peptides that have been implicated in a defined physiological or behavioral function. The third is a …
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Mass spectrometry-based investigations to characterize specific cell types within the brain
… abnormal brain function. Frequently, proteomics, peptidomics, and metabolomics experiments survey brain regions and attribute detected species to neurons. However, many other cell types are present, including astrocytes, oligodendrocytes, and microglia. It is also known that different cell types …
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Characterization of D-amino acid-containing neuropeptides from metazoa
… is already a complex measurement challenge. A peptidomics experiment that also seeks to determine D-amino acids present in such samples represents a much more difficult task than quantifying the amino acid configurations of several synthetic peptides, which is the sample type often used when …
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Sampling strategies for characterization of neuropeptides using mass spectrometry and functional implications into cell-cell signaling
… for mass spectrometric analysis. Here, the neuropeptidomics study of the SON resulted in the identification of 85 peptides, including 20 unique peptides from known prohormones. This study includes mass spectrometric analysis even from individually isolated magnocellular neuroendocrine cells, …
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Characterisation of human enteroendocrine cells in organoid models
… cell Ca²⁺ imaging, immunohistochemistry and peptidomics. GLU-Venus ileal organoids were first used to explore the mechanisms regulating GLP-1 release from human L-cells. We optimised protocols for the study and differentiation of EECs in human organoid culture, and used these to determine …
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Mass spectrometry-based characterization of cell-to-cell signaling molecules in the nervous system
Cell-to-cell signaling molecules are an important class of endogenous molecules which are responsible for cell-to-cell communication within organisms. Small molecules and peptides work as neurotransmitters and neuromodulators in the nervous system and play important regulatory roles in a wide range …
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A peptidomic investigation into enteroendocrine cells and islets of humans and mice
Enteroendocrine cells (EECs) of the gastrointestinal (GI) tract and islets of Langerhans (islets) of the pancreas are endocrine cells capable of sensing nutrient intake or the nutrient content of the blood and responding by secreting peptide hormones to control various physiological functions. This …