Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 12 of 12 for “"peptide-lipid"”.
-
Investigation of peptide-lipid interaction by fluorescence correlation spectroscopy
In this study a series of antimicrobial peptides (AMPs) called modified V4 (MV4s), was designed to have better solubility compared to original V4, which originated from a LPS-binding motif. The interaction between MV4s and different lipid model membranes was investigated using fluorescence …
-
Theoretical and computational modeling of peptide-lipid bilayer interaction studied by dynamic force spectroscopy
… projects that investigate different aspects of peptide-lipid membrane interactions. (1) A general theoretical approach is formulated for the quantitative description of the detachment force distribution, P(F), and the corresponding force dependent detachment rate, k(F), of a peptide from a lipid …
-
Electrospray Fundamentals and Non-Covalent Peptide-Lipid Interactions as Studied by Fourier Transform Ion Cyclotron Reonance Mass Spectrometry
… to study noncovalent associations between lipids and fusion peptides. Detailed binding specificities between selected phospholipids and model fusion peptides were investigated. Strong evidence has been compiled to demonstrate the importance of the initial hydrophobic interaction to the …
-
Design and Evaluation of Peptide Lipid-Associated Nucleic Acids (PLANAs) for siRNA and CRISPR/Cas9 Delivery and Protein Silencing
… challenge faced by siRNA, we have designed peptide lipid-associated nucleic acids (PLANAs) to enhance electrostatic interactions between siRNA and the cationic peptide and hydrophobic interactions with the phospholipid bilayer of the cell.</p> <p>The second approach is focused on utilizing …
-
Surface Acoustic Wave Nebulization as a Mass Spectrometry Ionization Source, Characterization and Application
… this method by successfully ionizing protein, peptide, lipid and other small molecules. The measured internal energy distribution of SAWN is lower compare to electrospray ionization (ESI). To improve the SAWN performance, a standing wave SAWN chip was designed and fabricated. The droplet size …
-
Hybrid Cyclic/Linear Peptides in a Multi-Component Lipid Structure as a siRNA Delivery System
… cells by crossing the negatively-charged phospholipid cell membrane. The goal of this project was to design hybrid cyclic-linear peptides as siRNA delivery system. We designed and synthesized hybrid cyclic-linear peptides [R5K]W5, [R6K]W6, and [R5K]W7 containing positively-charged arginine …
-
Nanoparticles Targeting at Inflammation Site
… manufacturing, this work explores polymeric, lipid-based, and peptide-modified nanocarriers tailored for chronic disease applications. In the context of osteoarthritis (OA)-associated pain, two classes of biodegradable polymeric nanoparticles—PEG-b-PCL and PLGA—were investigated for their …
-
Interaction of a helical peptide with membrane: Study of alamethicin
… at high concentration. It is a small size polypeptide (20 amino residues), and contains a large section of amphiphilic $\alpha$-helix, which is an often-encountered secondary structure motif in membrane active peptides and proteins. Its membrane-active functions, typical secondary structure, …
-
Analytical methods for the study of membranes and peptide-membrane interactions
… out to determine the stability and properties of lipid and peptide-lipid systems. Matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) and tandem mass spectrometry (MSMS) analyses were carried out to establish a validated protocol for the complete identification of …
-
Neutron scattering studies of water diffusion near the interface of model cell membranes
… largely depend on the interactions between peptide-lipid domains and the hydrating water. Therefore, the dynamics of the membrane-associated water and its interaction with embedded proteins remain some of the most fundamental issues in biological physics today. Single-supported lipid …
-
Structural basis for sulfatide recognition by Disabled-2
… outside-in signaling pathways. A Dab2-derived peptide, named the sulfatide-binding peptide (SBP), is the minimal unit of Dab2 to exert its function as a negative regulator of platelet activation and aggregation. The work of this thesis refines the model of Dab2 SBP binding to sulfatide and …
-
Deterministic design of peptide-membrane interactions
Antimicrobial peptides (AMPs) are present in virtually every multi-cellular organism and comprise an important component of the innate host defense system. Collectively, AMPs have broad spectrum and selective microbicidal effects. A general mechanism of AMP activity is destabilization of the …