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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 177 for “"peptide synthesis"”.
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Automated flow peptide synthesis
… nearly sixty years ago, batch solid phase peptide synthesis remains slow at minutes to hours per residue. Here we report a fully automated, flow based approach to solid phase polypeptide synthesis with amide bond formation in seven seconds and total synthesis times of forty seconds per …
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Design and synthesis of handles for solid-phase peptide synthesis and convergent peptide synthesis
The recent popularity of methods for solid-phase peptide synthesis that use the 9-fluorenylmethyloxycarbonyl (Fmoc) group for N<sup>α</sup>-amino protection has created a need for compatible anchoring linkages and handles. In an effort to develop mild new methods for use in solid-phase peptide …
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The prior thiol capture method for peptide synthesis
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, 1984
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The use of polymeric mixed anhydrides in peptide synthesis
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Advancements in polymer resins for solid-phase peptide synthesis
The use of polymeric supports in solid-phase peptide synthesis has allowed for the facile, rapid synthesis of short peptides in a one bead/one sequence manner. This approach allows for screening peptide libraries for binding and catalytic activities. Once hits are found, the sequence of the active …
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Studies on Solid-Phase Peptide Synthesis: The Synthesis of Glucagon
… stepwise-solid phase approach was studied in the synthesis of the peptide hormone glucagon. The choice of this molecule was based upon: (1) the unusual chemical structure; (2) biological significance. The main features of the strategy employed are: (1) the use of Biphenylisopropyloxycarbonyl group …
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Stereoselective glycosylation and chemical ligation-applications in oligosaccharide and peptide synthesis.
… ligation-applications in oligosaccharide and peptide synthesis.
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Investigation of Synthetic Proteins Produced via Automated Fast-Flow Peptide Synthesis
… Since the introduction of solid phase peptide synthesis (SPPS), the peptide community has endeavored to combine the convenience of flow chemistry with the iterative steps associated with peptide elongation in SPPS. Nearly one decade ago, members of the Pentelute lab envisioned and …
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Investigation and application of heterochiral proteins enabled by flow-based peptide synthesis
… are rare in protein structures. Solid-phase peptide synthesis (SPPS) and native chemical ligation enable the total chemical synthesis of proteins. Using these techniques, it is possible to design and study polypeptides foreign to Nature. Herein, I describe the investigation and application of …
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The immobilisation of Thermus strain Rt41A proteinase and its use in peptide synthesis
… specificity of the proteinase against various peptide p-nitroanilide substrates was investigated and compared with the results of Peek (unpublished results) and Cowan et al. (1987b) Rt41A proteinase has a preference for aromatic amino acids in the P₁ site but, will also cleave the pNA group …
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Solid phase peptide synthesis and TLR-5 activity analysis of pertide fragments from the Salmonella muenchen flagellin protein
… this protein are suggested to activate TLR-5: Peptide-N1, LQRVRELAVQ; Peptide-N2, LAVQSANGTNSQSD; and Peptide-C1, QNRFNSAITNLGNT. Here, we report the synthesis of these peptides and their activity against TLR-5 expressing HEK-293 cells. Our goal was to resolve the minimal region of flagellin …
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Molecular cloning, peptide synthesis and bioactive assessment of a skin defensive peptide, QUB-3117 from the skin secretion of Rana schmackeri
… various bioactivities. In this research, a novel peptide was isolated from the skin secretion of the Rana schmackeri by “Shotgun” Cloning. The mature peptide contains 29 amino acid residues (QUB-3117: DYMDTAKNAAKNMAAILLDKLKCKMTGGC). The functional group is carboxyl group at C-terminus. The peptide …
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Molecular cloning, peptide synthesis and bioactive assessment of a skin defensive peptide, QUB-3055 from the skin secretion of Hylarana latouchii
… antibiotics lose their effect. Antimicrobial peptides (AMPs) which are the most promising substances to be the next antimicrobial reagents are being continuously studied. In this research, we obtained QUB-3055 precursor-encoding cDNAs from skin secretions of Hylarana Latouchii by molecular …
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Molecular cloning, peptide synthesis and bioactive assessment of a skin defensive peptide, QUB-2658 from the skin secretion of <i>Limnonectes fujianensis</i>
… years, research on amphibian skin secretion peptides has become a hot topic for researchers. Antimicrobial peptides (AMPs) from amphibian skin secretion were found to have excellent broad-spectrum antibacterial activities and inhibit drug-resistant bacteria, which made AMPs a potential new …
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Substituted 2-ethyl-7-hydroxybenzisoxazolium salts in peptide synthesis and kinetic investigations on the aminolysis of N-acylamino acid p-nitrophenyl esters with various amino acid esters.
Massachusetts Institute of Technology. Dept. of Chemistry. Thesis. 1971. Ph.D.
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Fast flow biopolymer synthesis
… and application of fast flow solid phase synthesis for the preparation of peptides and phosphorodiamidate morpholino oligomers (PMOs), as well as the application of fast, reliable peptide synthesis to study non-natural protein folding and function. In the first chapter, solid supported …
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Development of New Synthetic Approaches to Biologically-Active Peptides
Synthetic peptides are widely used in drug research due to their high selectivity and efficaciousness, as well as in chemical biology as tools for modulating protein-protein interactions. While there are a large number of naturally occurring peptides, which can be used for the development of new …
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The synthesis characterization and application of novel N-ferrocenoyl peptide derivatives
… has been an increasing focus on the design and synthesis of electrochemical biosensors as they can lead to the analytical determination of biological and organic analytes. As amino acid and peptides play such a diverse role within biological systems incorporation of a redox active, chromophoric …
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Active Insulin Analogs That Do Not Require Refrigeration
… thus do not require refrigeration. Solid phase peptide synthesis techniques and the incorporation of backbone modified amino acids into peptide sequences will be used to design novel insulin analogs.
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The stereoselective synthesis of a novel aromatic peptide nucleic acid (APNA) oligomer
The synthesis of the thymine derivative (S)-50, was achieved in high enantiomeric purity (98% ee). The (R) enhanced enantiomer was also synthesized with moderate enantiomeric purity (46% ee). This acyclic pyrimidine analog ((S)-50) is a useful building block for the synthesis of a novel class of …
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