Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 40 for “"peptide substrates"”.
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Deoxyribozymes for peptide substrates: exploring the landscape of nucleophiles and electrophiles
… etc. Most of these reactions involve RNA or DNA substrates, where catalysis is facilitated by Watson-Crick base pairing between the substrate and the DNA catalyst. A major challenge in the field is to identify deoxyribozymes that can catalyze reactions involving non-nucleic acid substrates, such …
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Characterizing Multivalent Interactions Between Folded Protein Domains and Intrinsically Disordered Regions or Peptide Substrates
… domains and intrinsically disordered regions or peptide substrates.The subject of Chapter two is prolyl isomerase Ess1, which is an essential enzyme found in Saccharomyces cerevisiae. Ess1 regulates the transcription and co-transcriptional RNA processing by catalyzing the isomerization of …
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DNA-catalyzed covalent modification of amino acid side chains in tethered and free peptide substrates
… reactions involve oligonucleotide substrates. To expand the scope of substrates that can be catalyzed by deoxyribozymes, the main theme of the thesis is to seek DNA catalysts that covalently modify the side chains of amino acids. The thesis describes the numerous selection efforts …
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Kinase deoxyribozymes
… possibility to interact specifically with the substrates. Both ribozymes and deoxyribozymes have been identified to catalyze the phosphorylation of oligonucleotides. However, previous efforts to identify kinase deoxyribozymes to catalyze the phosphorylation of amino acid side chains were …
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Fluorescence resonance energy transfer-based biosensors for monitoring prostate specific antigen
… resonance energy transfer (FRET), several peptide substrates have been designed to respond to varying concentrations of PSA with a concomitant increase in fluorescence. In the near-infrared wavelength range, these fluorescent substrates can be imaged through thin sections of tissue to allow …
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Lysine and Glycyl-L-Sarcosine Absorption Across Ovine Forestomach Epithelium In Vitro
… studied during co-incubation with glycine and peptide substrates (each at 5 mM). Accumulation of Gly-Sar in omasal epithelium was greatest (P < .05) when Gly-Sar was present alone. Glycine inhibited (P < .05) Gly-Sar accumulation by 20%, whereas peptide substrates inhibited (P < .05) Gly-Sar …
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Development And Optimization Of The First High Throughput In Vitro Fret Assay To Characterize The Saccharomyces Cerevisiae Gpi-T
… appended glutathione-S-transferase (GST) domain. Peptide substrates for GPI-T were synthesized and modified to contain a pair of chromophores suitable for the development of a fluorescence resonance energy transfer (FRET) assay. GPI-T activity was observed as a time-dependent increase in …
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Chemical Methods for Cobalt-Catalysed C(sp3)–H Activation and Iodonium Salt-Mediated Peptide Functionalisation
… applied to the functionalisation of complex peptide substrates in dilute aqueous solutions, exhibiting high chemoselectivity with respect to peptide sidechains. Excellent conversions were achieved in five minutes at room temperature, demonstrating the potential of this process for …
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Impact of Neurodevelopmental Disorder-Associated Clinical Variants on the Catalytic Activity of KMT5B
… to assess enzyme activity on nucleosome and peptide substrates. While three of the four variants tested showed significant decreases in catalytic activity, one variant had a non-significant decrease. This differential KMT5B catalytic activity raises questions about the relationship between …
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Molecular substrate design for the selective adhesion, proliferation and differentiation of marrow connective tissue progenitors
… approach was applied to the molecular design of substrates for the selective adhesion, proliferation and differentiation of connective tissue progenitors (CTPs) from human bone marrow aspirates. The basic premise of the thesis is that integrin-specific adhesion peptides, when presented in a …
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Mechanistic studies of the AAA+ molecular motor ClpXP
… the ClpXP protease system. ClpX binds protein substrates via an amino acid sequence known as a tag, denatures them, and translocates them into the associated peptidase, ClpP. ClpX utilizes the energy from ATP hydrolysis to pull on bound substrates, destabilizing folded substrates and denaturing …
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Translocation and proteolysis by the energy-dependent protease ClpAP : coordination of conformational changes and active site chemistry
… ClpA) binds, unfolds, and translocates protein substrates into a protease component (e.g., ClpP) where degradation occurs. This thesis addresses the communication between ClpA and ClpP required to efficiently translocate and degrade protein substrates. Synchrotron hydroxyl radical footprinting …
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Hypervalent iodine reagents for protein functionalisation
… across a range of small proteins and polypeptides. In this work, we describe the development of a new generation of methionine-selective protein labelling reagents with improved reactivity and versatility, while enhancing the stability of the resultant protein conjugates. We have developed …
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Structural and Functional Characterization of B. Anthracis Udp-Glcnac 2-Epimerase and Salmonella Secreted Effector I
… in in vitro assays using small molecule and peptide substrates, but based on co-expression experiments with small Rho GTPases we were able to identify Cdc42 by mass spectrometry as a possible substrate undergoing deamidation at Gln61. The biological relevance of this result during infection …
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Studies of Bioactive Natural Products and Mechanism-Based Bioassays
… the development of new assays using shorter peptide substrates, small libraries of peptides have been synthesized and tested for their activity as Chk1 substrates. Several of the substrates synthesized displayed activity in the Chk1 assay.
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Investigations of Novel Mechanisms of Action for Anti-Bacterial and Anti-Cancer Agent Development
… in this approach is the low binding affinity for peptide substrates by kinases. We circumvented this problem of affinity by utilizing a covalent mode of binding and synthesized a potent non-peptide active-site directed irreversible compound that inhibits AKT. Further studies on this compound are …
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The design, synthesis and evaluation of selective, non invasive imaging agents for atherosclerotic plaque.
… via MRI. In order to achieve this, a library of peptide substrates designed to be substrate specific for either legumain or MMP-2/9 were synthesized. This was achieved via a combination of both solid and solution phase peptide synthesis. The first phase of the research study focused on the …
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Specificity of SH2 domains and protein tyrosine phosphatases
… for their use. Second, kinetic studies with pY-peptide substrates were used to attempt to uncover specificity determinants for three transmembrane PTPs. Finally. a general peptide affinity selection technique that was initially explored with the SH2 domains of SHP-2 was refined to define the in …
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Identification and Characterization of a Calmodulin -Binding Protein Related to a Family of ATPases Involved in Cell Division and Membrane Fusion
… plant CaM isoforms, their interaction with two peptide substrates was examined by fluorescence resonance energy transfer (FRET). FRET measurements between blue fluorescence protein fused with CaM-binding domains of CIP111 and green fluorescence protein fused with CaM isoforms indicate that they …
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Biochemical characterization of calmodulin isoforms and identification of calmodulin-binding proteins in Arabidopsis
… identical. In contrast, CaM-6M bound synthetic peptide substrates with lower apparent affinity than CaM-6. Because of the importance of NAD kinase as a model system for studying CaM structure and function relationships, a systematic series of experiments were performed to purify NAD kinase with …
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