Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 12 of 12 for “"peptide sequencing"”.
-
De novo peptide sequencing from matrix-assisted laser desorption/ionization-time of flight post-source-decay spectra
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2001.
-
The complexity of bioinformatics: techniques for addressing the combinatorial explosion in proteomics and genomics
… algorithms are applied to the problem of de novo peptide sequencing in Bottom Up proteomics by means of ultra-high-resolution mass spectrometry. Rather than detecting large numbers of less accurate fragment peaks as is presently typical in Bottom Up proteomics, detecting fragment ions at high …
-
Distinguishing Macrophage Activation States by Mass Spectrometry
… protein (CAPG) as source of the peptide, which could be validated through peptide sequencing through post-source decay experiments conducted on the tryptic fragments</p>
-
Development of biophysical and Mass Spectrometry Assays for APOBEC3 cytosine deaminases: discovery and validation of cnvalent and non-covalent ligands
… detect Reactive Molecules via Mass Spectrometry Peptide Sequencing), can be utilized to measure any covalent compound and its non-specific protein thiol reactivity through isotopic labeling. Appendix A describes a previously undescribed phenomenon where the A3A inactivating mutation E72Q …
-
Nutraceutical and functional food bioactive peptides in beef
… has received considerable attention. Bioactive peptides which are released during the hydrolysis or fermentation of food proteins or following digestion may exert beneficial physiological effects in vivo. The aim of this work was to isolate, characterise and evaluate Angiotensin-І-converting …
-
Development of a Transformation System for Caldariomyces Fumago and Expression of Heterologous Proteins
… amino acids including the 21 amino acid signal peptide. The putative mature protein consisted of 352 amino acids. The molecular weight of unglycosylated protein was calculated to be 38,270 daltons and the glycosylated protein was determined to be approximately 42,000 daltons. The …
-
Strings in proteomics and transcriptomics : algorithmic and combinatorial questions in mass spectrometry and EST clustering
… weighted string problem we discuss is de novo peptide sequencing, i.e., recovering the amino acid sequence of a sample peptide from tandem MS data, a specialized mass spectrometry experiment. We describe an algorithm which is an enhancement of a dynamic programming algorithm presented by Chen …
-
Discovery of an extracellular stress sensory protein in Beauveria bassiana and identification of photolyase encoding phr-1 sequences in five entomopathogenic fungi
… EC1 could increase heat tolerance in BS. De novo peptide sequencing revealed that the ECs were the same protein, but differed by EC1 being glycosylated. An expressed sequence tag from B. bassiana, encoding six peptides that were also found in the ECs, was identified in the public data base. This …
-
Characterization of D-amino acid-containing neuropeptides from metazoa
… become evident that certain residues within a peptide can be inverted to the D-form in an enzyme-mediated post-translational process, forming D-amino acid-containing peptides (DAACPs). DAACPs adopt different three-dimensional structures due to epimerization, and hence, different physicochemical …
-
Click Chemistry on DNA and Targeting RNA structure with Peptide Boronic Acids
… in biotechnology and diagnostics. Interest in peptides incorporating boronic acid moieties is increasing due to their potential as therapeutics/diagnostics for a variety of diseases such as cancer. The utility of peptide boronic acids may be expanded with access to vast libraries that can be …
-
Proteomic analysis of Pseudomonas putida F1 during growth on toluene-phenol mixtures
… P. and M proteins were subjected to N-terminal sequencing, and mass spectrometry, and results of these analyses were used in searches of the protein identities. Two different mass spectrometers were utilized: a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for mass …
-
Developmental and Cellular Functions of Delta-Catenin
… 6 of delta-catenin, was identified through peptide sequencing. Cleavage thus generates an amino- (1-816) and carboxyl-terminal (817-1314) fragment each containing about half of the central Armadillo-domain. I found that cleavage of delta-catenin both abolishes its association with cadherins, …