Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"peptide nucleic acids"”.
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Synthesis of aromatic peptide nucleic acids
The synthesis of two analogues of Aromatic Peptide Nucleic Acids (APNAs) monomers was successfully achieved. These Peptide Nucleic Acid analogues could be used as building blocks for the synthesis of oligomers with the DNA and RNA recognition properties of PNAs in addition to a more hydrophobic …
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Molecular Dynamics Study of Single Stranded Peptide Nucleic Acids
… replaced by a structurally homomorphous pseudopeptide chain consisting of <em>N</em>(2-aminoethyl)-glycine units. PNA binds strongly to both DNA and RNA. However, an analysis of the X-ray and NMR data show that the dihedral angles of PNA/DNA or PNA/DNA complexes are very different from those of …
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Functionalization of Peptide Nucleic Acids via post-synthetic click chemistry
Peptide Nucleic Acid (PNA) has shown great potential in molecular diagnostics, antisense/antigene therapy and nanotechnology. Like other synthetic nucleic acids and artificial analogues, PNA has been extensively modified to achieve better performance in these applications. To efficiently develop …
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Evaluation of the Protein Recognition Properties of Peptide Nucleic Acids
… synthetases (aaRS) to recognize the non-standard nucleic acid, PNA (peptide nucleic acid). PNA has immense potential in biomedical applications due to its increased thermostability and nuclease resistance over natural nucleic acids. PNA represents a superior alternative to natural nucleic acids in …
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EMISSIVE PEPTIDE NUCLEIC ACIDS (PNAS): POTENTIAL TOOLS FOR INNOVATIVE DIAGNOSTICS AND THERAPEUTICS
ABSTRACT I. Introduction and aim of the thesis Peptide nucleic acids (PNAs)1 are synthetic mimics of natural nucleic acids, in which the chiral and negatively charged sugar-phosphate backbone is replaced by achiral and neutral N-(2-aminoethyl)-glycine (aeg) units, where the four nucleobases …
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Characterization, toxicity, and biological activities of organometallic compounds and peptide nucleic acids for potential use as antimicrobials
… molecules, organometallic compounds (OMC) and peptide nucleic acids (PNA). Organometallic compounds show promise as antimicrobial drugs because of their structural difference from conventional antibiotics and antimicrobials, and because of the ability to "tune" their chemical and biological …
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The stereoselective synthesis of a novel aromatic peptide nucleic acid (APNA) oligomer
… of a novel class of oligomers, the aromatic peptide nucleic acids (APNA). The APNA tetramer 70 was prepared from the amino acid monomer (S)-50 using classical peptide synthesis. Chemical shift differences between the $\sp1$H NMR of monomer (S)-57 and tetramer 70 were observed which suggested …
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Discovery of PSMA-Specific Peptide Ligands and Development of A Noncovalent Method to Attach Chemical Moieties to siRNA
… (Prostate Specific Membrane Antigen)-specific peptide ligands using phage display and employ peptide nucleic acid as a complementary linker to attach chemical moieties to siRNA. In Chapters 1 and 2, we introduced and discussed the background of prostate cancer chemotherapy, PSMA specific …
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Automated flow peptide synthesis
… nearly sixty years ago, batch solid phase peptide synthesis remains slow at minutes to hours per residue. Here we report a fully automated, flow based approach to solid phase polypeptide synthesis with amide bond formation in seven seconds and total synthesis times of forty seconds per …
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Design, Synthesis And Characterization Of Lipidated Pna-Peptide Conjugates As Potentialtherapeutic And Diagnostic Reagents
Peptide Nucleic Acids: PNAs), are an ideal choice for antisense and antigene tools and probes because of their stability and high binding affinity, but have limited application as therapeutic and diagnostic agents because of poor membrane permeability. To increase membrane permeability, we …
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Development of Shell-Crosslinked Knedel-like Nanoparticles as Intracellular Delivery Vehicles and Gene Regulation Agents
… DNA into the cell to allow the DNA to function. Peptide nucleic acids: PNAs) were also effectively transported into the cell by covalent conjugation to or electrostatically complexation with the cSCKs. Finally, the cSCKs were shown to be able to form hierarchical nanoscale structures with anionc, …
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Protective antigen-mediated delivery of biomolecules
… a wide variety of natural and non-natural peptides and proteins. Despite the significant progress made with the LFN/PA delivery platform, some aspects including cargo selection and targeting still remain limited. In the first part of the thesis, we greatly expand the application of the …
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Investigating novel treatment approaches to combat Clostridioides difficile
… has been to develop targeted therapeutics called peptide nucleic acids (PNAs). PNAs are antisense agents capable of inhibiting gene expression in bacteria. In this study, antisense inhibition of the RNA polymerase subunit gene (rpoA) of C. difficile was found to be bactericidal for the pathogen …
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DESIGN AND DEVELOPMENT OF RNA-BASED DRUGS USING MOLECULAR DYNAMICS SIMULATIONS AND ARTIFICIAL INTELLIGENCE METHODS
… advanced chemical modifications, such as Xeno Nucleic Acids (XNAs) and 2-OH modifications, along with specialized delivery systems, including viral vectors and lipid nanoparticles (LNPs), have been introduced. The design of these advanced ligands increasingly relies on computational approaches, …
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Automated Flow Synthesis of Biomacromolecules
… (PMOs), mirror image proteins (D proteins), and peptide nucleic acids (PNAs) are functional but require individualized attention and time-intensive effort. As a result, unlike easily accessible biopolymer classes, widespread application of PMOs, D proteins, and PNAs in biotechnological workflows …
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Analyzing the role of a cellular mechanism in HIV infection and Evaluation of novel antiviral compounds
… the nuclear membrane. Furthermore, we tested peptide nucleic acids (PNAs) as new compounds to inhibit viral spread at different stages of the lifecycle. PNAs have been developed recently and dispose of several features to be a powerful tool for a novel antisense approach. We synthesized a …
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Ultrasensitive methods for the detection of biomolecular systems at the micro/nano scale
… DNA sequences detection, due to the relevance of nucleic acid analysis in many areas, including clinical diagnostics, environmental monitoring and food-quality control. However, diagnostic tools used for the detection of nucleic acids need to fulfil specific requirements in term of sensitivity, …
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Establishment of a Long Term Cell Culture Model for Testing Anti-Infectives against Mycobacterium avium subsp. paratuberculosis
Mycobacterium avium subsp. paratuberculosis (MAP) is a very slow growing bacterium that is the causative agent of Johne's disease (JD) in ruminants and has long been suggested to be associated with complications of Crohn's disease (CD) in humans. Although there is no direct evidence that MAP is the …
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Förster Resonance Energy Transfer-Based Quantification of D-2-Hydroxyglutarate & Locked Nucleic Acid Loop-Mediated Isothermal Amplification Mediated Detection of IDH1-R132 Single Nucleotide Variants in Glioma
<p>Isocitrate dehydrogenase 1 (<em>IDH1</em>) is a biomarker in gliomas which provides valuable information on patient prognosis and treatment. Targeted therapies including maximal surgical resection and pharmaceutical mutant IDH inhibitors offer life extending benefits; however, currently …