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Showing 1 to 20 of 37 for “"peptide nucleic acid"”.
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Synthesis of Novel Cysteine Peptide Nucleic Acid (CPNA)
… gives birth to a new class of oligomers called Peptide Nucleic Acid (PNA). We herein report cysteine based PNA called CPNA.
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DNA enzymes for peptide-nucleic acid conjugation and for lysine methylation
… identification of new enzymes favors the use of nucleic acids over proteins for several reasons. First, nucleic acids can be amplified by natural enzymes whereas proteins cannot be amplified in any way. Second, the number of possible sequences is smaller for nucleic acids (4n, where n is the …
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The stereoselective synthesis of a novel aromatic peptide nucleic acid (APNA) oligomer
… of a novel class of oligomers, the aromatic peptide nucleic acids (APNA). The APNA tetramer 70 was prepared from the amino acid monomer (S)-50 using classical peptide synthesis. Chemical shift differences between the $\sp1$H NMR of monomer (S)-57 and tetramer 70 were observed which suggested …
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Synthesis of Peptide Nucleic Acid (PNA) and Entrapment of PNA into Liposomes
Peptide Nucleic Acid (PNA) is a synthetic pseudopeptide nucleic acid analogue in which the deoxyribose phosphodiester backbone of DNA is replaced by a pseudo-peptide composed of N-(2 aminoethyl)glycine units, resulting in an achiral and uncharged DNA mimic. PNA is chemically stable and resistant to …
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A Rapid Colorimetric Peptide Nucleic Acid Loop-Mediated Isothermal Amplification Assay for the Detection of the IDH1 Mutation in Glioblastoma
… We designed a novel colorimetric peptide nucleic acid loop-mediated isothermal amplification (PNA-LAMP) method that rapidly detects the <em>IDH1</em> R132H mutation in GBM. PNA-LAMP amplifies target DNA under isothermal conditions with high specificity and speed. The PNA prevents …
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DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF BACTERIAL AND VIRAL PATHOGENS USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION COUPLED WITH A PEPTIDE NUCLEIC ACID AND GOLD NANOPARTICLE REPORTER SYSTEM
… amplification (LAMP) reaction followed by a peptide nucleic acid (PNA) probe and gold nanoparticle (AuNP) detection system that results in a colorimetric assay. By employing modified LAMP primers, the target-dependent, co-amplification of a novel, sequence is generated, which serves as the …
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Evaluation of the Protein Recognition Properties of Peptide Nucleic Acids
… synthetases (aaRS) to recognize the non-standard nucleic acid, PNA (peptide nucleic acid). PNA has immense potential in biomedical applications due to its increased thermostability and nuclease resistance over natural nucleic acids. PNA represents a superior alternative to natural nucleic acids in …
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Synthesis of aromatic peptide nucleic acids
The synthesis of two analogues of Aromatic Peptide Nucleic Acids (APNAs) monomers was successfully achieved. These Peptide Nucleic Acid analogues could be used as building blocks for the synthesis of oligomers with the DNA and RNA recognition properties of PNAs in addition to a more hydrophobic …
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Targeting RNA With Small Molecules: SL3 of HIV -1 Psi-Rna
… micromolar affinities for SL3 RNA including two peptide-nucleic acid conjugates that disrupted NCp7-SL3 complex with sub-micromolar inhibition constants.
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Nucleic acid denaturation at an electrode surface
… from the electrode surface. It was found that peptide nucleic acid (an uncharged analogue of DNA) could be denatured electrochemically. Local pH changes were also ruled-out as a possible mechanism through experiments in which the surface pH and electrochemical melting were monitored …
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Targeted 177 Lu antisense radiotherapy of B-cell non-Hodgkin's lymphoma
… a [superscript 177]Lu-labeled bcl-2 antisense peptide nucleic acid (PNA)-peptide conjugate designed for dual modality NHL therapy, consisting of a radiopharmaceutical capable of simultaneously down-regulating apoptotic resistance and delivering cytotoxic internally emitted radiation. In vitro …
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PNA-Ligand Bioconjugates as Potential Building Blocks for Sequence-Specific, Metal-Mediated DNA-/RNA-Cleavage
… its substitution. As a recognition domain, PNA (peptide nucleic acid), a DNA mimic with a pseudopeptide backbone was chosen, and the synthesis of monomer building blocks was optimized. PNA oligomers with terminal ligand substitution were developed, and their DNA hybridization behaviour was …
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G4-RESOLVASE 1 TIGHTLY BINDS AND CATALYTICALLY UNWINDS UNIMOLECULAR G-QUADRUPLEX STRUCTURES WITH IMPLICATIONS FOR GENE TRANSCRIPTION AND TELOMERE BIOLOGY
… for any known G4-binding protein. Using a novel peptide nucleic acid (PNA) trap assay, we show that G4R1 catalyzes unwinding of unimolecular G4-DNA into an unstructured state. Additionally, we provide strong evidence for the presence of unimolecular G4-structures in the promoter of the Yin Yang 1 …
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Variation in chromosomal aberration induction in differing ATM and ASPM genotypic backgrounds detected via a modified PNA FISH technique
A modified PNA (peptide nucleic acid) FISH (fluorescence in situ hybridization) method has been developed for efficient quantitative and qualitative analysis of chromosomal aberration formation. PNA FISH was used to detect a heightened sensitivity in mouse fibroblasts in response to ionizing …
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Ortsaufgelöster Aufbau von DNA-Nanostrukturen auf Glasoberflächen
… DNA-Struktur wurde durch die Bindung von PNA (peptide nucleic acid) an den DNA-Doppelstrang erbracht. Diese PNA-Bindung stellt ihrerseits ein funktionales Strukturelement im Nanometermaßstab dar und wird als Nanostrukturbaustein verstanden.
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RAPID COLORIMETRIC IDENTIFICATION OF ESCHERICHIA COLI SHIGA TOXINS 1 AND 2, AS WELL AS EAE AND AGGR, USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION FOLLOWED BY PNA/AUNP DETECTION
… To make the LAMP assay more specific, a peptide nucleic acid (PNA) probe complementary to either the Shiga toxin 1 or 2 genes, <em>eae</em> or <em>aggR</em> was introduced, along with gold nanoparticles (AuNP). The PNA-AuNP detection system does not depend on pH change, rather an …
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DNA based biosensors for environmental and medical applications
… Among the sequence probes, modified locked nucleic acid (LNA) and the peptide nucleic acid (PNA) were used. In this case the screen printed electrode Abstract iv was used only as transducer while the hybridization assay was conducted onto paramagnetic micro beads. The genosensor was used for …
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Discovery of PSMA-Specific Peptide Ligands and Development of A Noncovalent Method to Attach Chemical Moieties to siRNA
… (Prostate Specific Membrane Antigen)-specific peptide ligands using phage display and employ peptide nucleic acid as a complementary linker to attach chemical moieties to siRNA. In Chapters 1 and 2, we introduced and discussed the background of prostate cancer chemotherapy, PSMA specific …
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Targeting oncogenic microRNAs in malignant germ cell tumours.
… (pri-miRNA) of each cluster using locked nucleic acid (LNA)-based gapmeRs and targeting the mature miRNAs with peptide nucleic acid and LNA-based inhibitors. Targeting the mature microRNAs using LNA-based inhibitors resulted in a significant and reproducible negative growth phenotype. Gene …
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