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Showing 1 to 6 of 6 for “"peptide ligation"”.
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Structure and regulation of yeast glycogen synthase
… acids near the C-terminus of the enzyme’s polypeptide chain. We studied isoform-2 of yeast glycogen synthase as a model to study the structural and molecular mechanisms that underlie the regulation of the eukaryotic enzymes and our primary tools of investigation were macromolecular X-ray …
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Sortase as a Tool in Biotechnology and Medicine
… protein therapeutics. </p><p>We utilized native peptide ligation —a well-known function of sortase A— to attach a small molecule drug specifically to the carboxy-terminus of a recombinant protein. By combining this reaction with the unique phase behavior of elastin-like polypeptides, we developed …
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Kupplung der DNA-Methyltransferase M.SssI mit Triplehelix-bildenden Oligodesoxynucleotiden
… M.SssI was coupled with TFO by native chemical peptide ligation (NCPL), thiazolidine ligation, expressed protein ligation (EPL) and heterobifunctional crosslinking. The different methods were optimised and compared with one another. Furthermore, the variant M.SssI-C141S, which doesn’t possess a …
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Enzymatic crosslinking of dynamic hydrogels for in vitro cell culture
… reversible stiffening through SrtA-mediated peptide ligation were established. Specifically, poly(ethylene glycol) (PEG)-peptide hydrogels were fabricated with peptide linkers containing pendent SrtA substrates. The hydrogels were stiffened through incubation with SrtA, whereas gel softening …
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Prebiotic photoreduction and polymerization of cysteinyl peptides.
Cysteinyl peptides likely played an important role in the prebiotic synthesis of cofactors, such as iron-sulfur clusters. However, cysteinyl peptides must be reduced in order to coordinate iron-sulfur clusters. Mixtures of ferric ions and cysteinyl peptides leads to the reduction of ferric to …
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Probing the roles of metal binding ligands in cupredoxins: incorporating nonproteinogenic amino acids into azurin and CuA Azurin
Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2010-05-13T19:03:20Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Clark_Kevin.pdf: 14900477 bytes, checksum: 901cc6f1c2f90be4a62e58094e6f0b6d (MD5)