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Showing 1 to 5 of 5 for “"peptide ligation"”.
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Structure and regulation of yeast glycogen synthase
… acids near the C-terminus of the enzyme’s polypeptide chain. We studied isoform-2 of yeast glycogen synthase as a model to study the structural and molecular mechanisms that underlie the regulation of the eukaryotic enzymes and our primary tools of investigation were macromolecular X-ray …
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Sortase as a Tool in Biotechnology and Medicine
… protein therapeutics. </p><p>We utilized native peptide ligation —a well-known function of sortase A— to attach a small molecule drug specifically to the carboxy-terminus of a recombinant protein. By combining this reaction with the unique phase behavior of elastin-like polypeptides, we developed …
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Enzymatic crosslinking of dynamic hydrogels for in vitro cell culture
… reversible stiffening through SrtA-mediated peptide ligation were established. Specifically, poly(ethylene glycol) (PEG)-peptide hydrogels were fabricated with peptide linkers containing pendent SrtA substrates. The hydrogels were stiffened through incubation with SrtA, whereas gel softening …
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Prebiotic photoreduction and polymerization of cysteinyl peptides.
Cysteinyl peptides likely played an important role in the prebiotic synthesis of cofactors, such as iron-sulfur clusters. However, cysteinyl peptides must be reduced in order to coordinate iron-sulfur clusters. Mixtures of ferric ions and cysteinyl peptides leads to the reduction of ferric to …
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Probing the roles of metal binding ligands in cupredoxins: incorporating nonproteinogenic amino acids into azurin and CuA Azurin
Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2010-05-13T19:03:20Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Clark_Kevin.pdf: 14900477 bytes, checksum: 901cc6f1c2f90be4a62e58094e6f0b6d (MD5)