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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"peptide ligands"”.
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Peptide ligands that bind to staphylococcal enterotoxin B (SEB)
… aureus fermentation broth. Inexpensive, robust ligands with high affinity would be suitable replacements for antibodies in biosensors for the detection of SEB. Affinity adsorption processes using short peptides as ligands show great promise in purifying and detecting proteins in comparison with …
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The Development Of Peptide Ligands To Target H69 Rrna
<p>ABSTRACT</p> <p>THE DEVELOPMENT OF PEPTIDE LIGANDS TO TARGET H69</p> <p>by</p> <p>DANIELLE NICOLE DREMANN</p> <p>December 2015</p> <p>Advisor: Prof. Christine S. Chow</p> <p>Major: Chemistry (Biochemistry)</p> <p>Degree: Doctor of Philosophy </p> <p>In the development of peptide ligands to …
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Affinity Chromatographic Purification of Human Immunoglobulins by Hexamer Peptide Ligands.
North Carolina State University Theses Chemical Engineering.
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Quantitative analysis of the T cell receptor signaling network in response to altered peptide ligands
… receptor, the T cell receptor, bind to multiple ligands resulting in different cell fates, based in part on the avidity of the ligand. To address the question of ligand affinity discrimination in T cells, several T cell lines, both mouse and human, were screened for their ability to exhibit …
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Discovery of PSMA-Specific Peptide Ligands and Development of A Noncovalent Method to Attach Chemical Moieties to siRNA
… (Prostate Specific Membrane Antigen)-specific peptide ligands using phage display and employ peptide nucleic acid as a complementary linker to attach chemical moieties to siRNA. In Chapters 1 and 2, we introduced and discussed the background of prostate cancer chemotherapy, PSMA specific …
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Design of Peptide Ligands for 14-3-3ε, and Exploring the Role of Residue Types in Ligand Recognition by 14-3-3 Proteins
… group developed two novel, 14 amino acid residue peptides; Ac-CDC25A(173-186)-NH2 (pS), and Ac-CDC25A(502-515)-NH2 (pT), corresponding to two binding regions of CDC25A to 14-3-3 proteins. Both pS and pT bind 14-3-3ε, and induce cell death of cSCC cells, albeit at a high IC50. In the work presented …
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Protein/Peptide Recognition Modules in Cellular Signaling and HIV Pathogenesis
<p>The peptide-recognition domains play a key role in Eukaryotic signal transduction by mediating sequence-specific interactions with their protein/peptide ligands. In this thesis, I illustrate in molecular detail the recognition mechanisms utilized by three peptide-recognition domains: SH2 (Src …
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Conserved Non-Pocket Interactions Drive The Diversity of Peptide Presentation By Mhc Class I Molecules
… or transformed cells through recognition of peptides presented on human leukocyte antigen (HLA) molecules. A thorough understanding of peptide-HLA interactions is needed for improvement of CTL-based immunotherapies. We observed that aspartic acid (D) and glutamic acid (E) at peptide position …
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Discovery of Peptide and Aptamer Ligands for Targeted Drug Delivery to Hepatic Stellate Cells
… identify Hepatic Stellate Cells (HSCs) targeting ligands using phage-display library biopanning and systematic evolution of ligands by exponential enrichment (SELEX). These HSC-specific ligands can be used for diagnosis and therapy of liver fibrosis. The mechanism of fibrogenesis and potential …
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In vitro and in vivo approaches to tumor targeting by phage display
… used as a target for the search for protein and peptide ligands that may be useful as therapeutic and diagnostic agents for treating and imaging cancer. In the work described here, I used several commonly used in vitro, ex vivo, and in vivo phage display techniques and binding assays in an …
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Antigen Recognition by an Alloreactive T Lymphocyte Clone
… ability of CTL 2C to recognize thiol-modified peptide variants is analyzed. A method for the synthesis and screening of thiol-modified peptides that are recognized by T cells is presented. Chapter three continues the peptide variant approach with examination of the ability of clone 2C and its …
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Novel methods in computational analysis and design of protein-protein interactions : applications to phosphoregulated interactions
… to the study of the interactions of phosphopeptides with phosphopeptide-binding domain interactions. A novel protein-protein interaction type, the action-at-a-distance interaction, is described in the complex of the TEM1 P-lactamase with the 3-lactamase inhibitor protein (BLIP). New …
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The mechanobiology of the T-Cell Receptor: Structurally connecting catch bonds with ligand binding and triggering
… investigate how the information encoded in the peptide is decoded mechanically by the TCR and what is the structural role of catch bonds. It is proposed that TCRs uses catch bonds to determine whether or not the presented antigen is a threat. It is demonstrated for the first time that catch …
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Phage Display to Identify Peptides Binding to or Penetrating the Mouse Zona Pellucida
The objective of this study was to identify peptide ligands, using phage display techniques, which bind sites on mouse embryos, ovaries, cytoplasmic membranes and/or intracytoplasmic components. Specifically, M13 coliphage 7-mer, 12-mer and 15-mer random peptide libraries were used separately for …
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Design synthesis and biological evaluation of non-peptide neuropeptide FF ligands
Neuropeptide FF is an endogenous RF-amide with two receptor subtypes originally described as having anti-opioid characteristics. While peptide work helped to elucidate key features for targeting the subtypes of the neuropeptide FF receptor, non-peptide small molecules offer a more refined tool to …
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A T cell receptor transgenic approach to the study of thymocyte development and autoimmune disease
CDS+ T lymphocytes recognize short peptide fragments when bound to self-class I major histocompatibility proteins. On a mature lymphocyte, such recognition results in activation of the T cell. On an immature thymocyte, such recognition may result in either positive selection, allowing the cell to …
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Peptide microarrays for the discovery of cell-ligand interactions that direct cell state
… The aim of this project is to develop a peptide array to screen for specific cell-ligand interactions. We synthesize a library of peptides that are derived from extracellular matrix proteins and serve as a highly scalable synthetic microenvironment. We demonstrate that by displaying …
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The Identification and Pharmacological Characterisation of Novel Apelin Receptor Agonists In Vitro and In Vivo
… coupled apelin receptor and two endogenous peptide ligands, apelin and elabela. It is implicated as a potential therapeutic for a number of diseases; however, the endogenous peptides are limited by half-life and bioavailability. This study aims to identify and pharmacologically characterise …
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Studies on the use of peptide auxiliaries in the meso-desymmetrization of epoxides, and the kinetic resolution of secondary alcohols
This thesis reports on the use of amino acids and peptides as chiral ligands/catalysts in two asymmetric processes: Meso-desymmetrization of an epoxide and the kinetic resolution of secondary alcohols. Chapter 1 comprises a literature review, which gives a general overview of methods of asymmetric …
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Investigating the role of the cardiac apelin receptor using human embryonic stem cell derived-cardiomyocytes.
… coupled apelin receptor and its two endogenous peptide ligands, apelin and Elabela, have emerged as key regulators of cardiovascular development, physiology and disease. It has therefore been suggested that targeting the apelin receptor therapeutically may be beneficial for the treatment of a …
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