Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"peptide array"”.
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An investigation into the role of Bcl-3 in toll-like receptor signalling
… the interaction between Bcl-3 and p50 and using peptide array technology. Key amino acids required for the formation of the p50:Bcl-3 immunosuppressor complex were identified. Furthermore, we demonstrate for the first time that interaction between Bcl-3 and p50 is necessary and sufficient for the …
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Development of an Assay to study the Kinetics of HIV-1 Capsid Uncoating
… antibody targeting a capsid internalised repeat peptide array, and 3) a split-luciferase system tagging an internalised component of the capsid core. Fluorescent labelling of both the CA and IN proteins enabled the sensitive and specific analysis of uncoating in response to both restriction …
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Ehrlichia chaffeensis Type 1 Secreted Effector TRP47 is a Novel SUMOylated Nucleomodulin
… nuclear localization. Using a microfluidic chip peptide array, a SUMOylation site in the TRP47 N-terminal domain was identified. A SUMO-2 modification was further confirmed using an in vitro SUMOylation assay with recombinant TRP47, and native polySUMOylated TRP47 was immunoprecipitated from E. …
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Peptide microarrays for the discovery of cell-ligand interactions that direct cell state
… cell phenotype, and recent advances in microarray technology has allowed the systematic investigation of a large combinatorial space of material properties. As we gain new insights on the effects of chemical structures and physical properties, controlling the interaction between these various …
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Characterizing The Recognition Motif and Novel Substrates of Carm1
… addressed. Here we describe the use of Oriented Peptide Array Libraries (OPALs) to methodically dissect the preferred methylation motifs for three of these enzymes – PRMT1, Coactivator associated arginine methyltransferase 1 (CARM1) and PRMT9. The OPAL platform can also be used to gauge the …
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Regulation of cancer progression by hepatitis B virus X protein (HBx) and HBxAP/RSF1
… Energy Transfer) assay. 37 5. Modified histone peptide array. 37 6. Purification of RSF1. 37 7. Protein microchip array. . 37 C. RESULTS 39 1. RSF1 is overexpressed in HBV-related advanced HCC. 39 2. RSF1 depletion induces premature separation of sister chromatids. 39 3. The centromeric …
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Biochemical and structural characterization of Sirtuins from mammals and Thermotoga maritima
… resveratrol can directly bind to Sirt1. Using a peptide array harboring all known mammalian acetylation sites several novel substrates for Sirt1 were identified and shown that resveratrol and other small molecules can modulate Sirt1 dependent deacetylation of substrate peptides and that the …
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Mise au point d'une forme stable de l'allergène Der p 3 de Dermatophagoides pteronyssinus pour le diagnostic rapide et le développement de nouvelles approches d'immunothérapie de l'allergie aux acariens
… de cette thèse, nous avons pu montrer que le propeptide de proDer p 3 affecte l’allergénicité de Der p 3. Par une étude in vitro, nous avons prouvé par ELISA, par les tests de dégranulation des basophiles de rat humanisés et d’activation des basophiles humains, que proDer p 3 est moins bien …
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Molecular and cellular aspects of the SREBP pathway
… SREBP1 and SREBP2 CTDs and probing of a SCAP peptide array with the CTDs. Results from the SREBP2 probing were difficult to interpret due to insolubility issues with the protein, however, probing with SREBP1 revealed five potential binding sites which were detected reproducibly. Further …
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Identification of cellular signaling events dysregulated in Huntington’s disease.
… development of a high throughput screening tool, peptide arrays. The arrays were exposed to lysates from cells / tissues where in the kinases from them phosphorylate the peptide spotted on the arrays. The degree of phosphorylation is measured for each spot on the array and compared to the controls …
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Using prophylactics to improve resistance against Enterobacteriaceae in chickens
… shifts in the ileum, the chicken kinome peptide array was used to measure global changes in phosphorylation networks. Generally, SPORE treatment induced drastic shifts in host immunometabolism. Furthermore, these changes were associated with Salmonella resistance, as TH17 cell …
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Disrupting the inhibitory Kappa B kinase (IKK) - Aurora A axis in cancer
… and IKKβ with Aurora A were confirmed. Utilising peptide array technology, it has been possible to 2 further elucidate the nature of the interaction between Aurora A and IKKα/β. The interaction of Aurora A and IKKα/β was mapped to two key regions of the IKKs, the kinase domain and the NEMO binding …
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Charakterisierung von Borna Disease Virus-spezifischen Antikörpern in Human- und Tierseren
… mit Hilfe von speziell synthetisierten Peptidarrays, welche die BDV-Proteine N, P und M repräsentieren, die Spezifität BDV-reaktiver Antikörper weiter zu charakterisieren. So konnte gezeigt werden, daß die BDV-reaktiven Antikörper in den untersuchten Humanseren ein komplexes Muster an …
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Integrin-ligand interactions and cytokine production by monocytic cells
… and αXβ2, from the β2 family of integrins. Using peptide array technology, we identified set of overlapping peptides derived from the soluble CD23 sequence that interact with integrins expressed on the surface of monocytic cells and with purified αVβ3 and αVβ5 integrins in in vitro Biacore assays. …