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Showing 1 to 17 of 17 for “"penicillin-binding protein"”.
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Functional Studies of Penicillin-binding Protein 1 in Bacillus subtilis
The penicillin-binding proteins (PBPs) synthesize and modify peptidoglycan (PG), the main structural element of the bacterial cell wall. PBPs and PG synthesis are highly conserved in all bacteria and both have been important targets for antibiotic and antibacterial development. In the Gram positive …
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Characterization of two Bacillus subtilis penicillin-binding protein-coding genes, ykuA (pbpH) and yrrR (pbpI)
Penicillin-binding proteins (PBPs) are required in the synthesis of the cell wall of bacteria. In Bacillus subtilis, PBPs play important roles in the life cycle, including both vegetative growth and sporulation, and contribute to the formation of the different structures of vegetative cell wall and …
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The Influence of ampC Expression and Penicillin Binding Protein Binding on the Variation of ß-lactam Induction Potential in Three Genera of Enterobacteriaceae
… carbapenems. The interaction of ß-lactams with penicillin binding proteins (PBPs) disrupts cell wall synthesis and in the case of some ß-lactams, initiates the ampC induction pathway. Several factors are involved in the induction of ampC in all genera including the interaction of a ß-lactam …
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Characterization of Peptidoglycan, and the Enzymes that Synthesize it, in Borrelia burgdorferi and Insights into the Peptidoglycan of Other Pathogenic Borrelia
… provide partial characterization of a putative penicillin-binding protein in B. burgdorferi — a class of highly conserved PG synthesis enzymes present in the vast majority of bacteria. Collectively, the work in this thesis furthers our understanding of the structure, function, and synthesis of …
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The postantibiotic effect of the carbapenem antibiotics on gram-negative bacteria
… by viable count. Thus, the recovery of the penicillin binding protein target enzymes appears to be the mechanism behind carbapenem PAE in P. aeruginosa.
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The penicillin-binding proteins of Bacilli
A study of the penicillin-binding proteins (PBPs) in the membranes of various species of Bacilli by dodecylsulphate/polyacrylamide gel electrophoresis followed by detection of the PBPs by fluorography has shown the presence of 3-5 such proteins depending on the species. Labelling of growing cells …
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Insights Into Determinants That Contribute to Colonization, Virulence and Antibiotic Resistance In Enterococci
… start codon downregulates EbpA translation and protein levels, diminishing biofilm and binding abilities of <em>E. faecalis</em>, as compared to an engineered ATG codon. My studies also extended to the ortholog of Ebp in <em>E. faecium</em>, known as the <em>E. faecium</em> <em>p</em>ili (Emp), …
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Studies on the mode of action and beta-lactamase inhibitory properties of novel oxapenem compounds
… cells in E. coli DC0, indicating inhibition of penicillin-binding protein 2 (PBP2). The PBP affinity profile of AM-112 was examined in isolated cell membranes of E. coli DC0, S. aureus NCTC 6571, Enterococcus faecalis SFZ and E. faecalis ATCC 29213, in competition with a radiolabelled …
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Mechanisms of resistance to β-lactam antibiotics in Streptomycetes
… was to underpin the mechanism of resistance to penicillin G in isolated strains of streptomycetes that exhibit elevated resistance to penicillin G and to characterise these organisms. Moreover, to investigate the interaction between penicillin G and PBPs in Streptomyces strains and investigate …
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Studying the riboregulatory role of Hfq in Klebsiella pneumoniae using CLASH (crosslinking, ligation and sequencing of hybrids)
… expression before translating the mRNAs into proteins. PTR mechanisms involve rewiring the gene expression using small non-coding RNAs (sRNA) and RNA binding proteins (RBPs). In this study, I investigated Hfq, an RBP in a Gram-negative nosocomial pathogen Klebsiella pneumoniae (Kp), using a …
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Attività del ceftobiprole contro Enterococcus faecalis; sviluppo della resistenza, interazione con molteplici proteine leganti la penicillina a bassa reattività (PBPs) e alterazioni della PBP4
… e ad alterazioni della sequenza genica di una Penicillin-Binding Protein (PBP) a bassa affinità, nota come PBP4, nonché al più raro meccanismo di produzione di beta-lattamasi. Il ceftobiprolo, una cefalosporina di nuova generazione, è considerata una valida opzione terapeutica contro …
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Synthesis of Structurally Diverse N-Substituted Quaternary Carbon Containing Small Molecules and Their Application as Novel Starting Points for Drug Discovery
… novel biological targets (CFI25, Activin A and Penicillin-binding protein 3). Utilising the modular nature of the synthetic route developed, derivatives of each hit were readily generated interrogating several synthetic exit vectors, including the N-substituted quaternary centre. In turn, these …
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Requirements for Compartmentalization of Penicillin-Binding Proteins during Sporulation in Bacillus subtilis
Penicillin-binding proteins (PBP's) are membrane-associated enzymes involved in the polymerization of peptidoglycan. PBP's are divided into three classes based upon their molecular weights and functional domains. Gene expression is regulated in the two differentiated cells in Bacillus subtilis, the …
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INVESTIGATION OF ANTIBIOFILM AND ANTIVIRULENCE ACTIVITY OF 5-FLUOROCYTOSINE IN ESCHERICHIA COLI
… DNA fragment containing N-terminal domain of the penicillin-binding protein 1b (PBP1b), involved in peptidoglycan synthesis, and D8B36_18480, an unknown protein, restored curli production despite presence of 5-FC. Deleting PBP1b-encoding gene mrcB in E. coli increased curli gene expression, while …
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Construction of plasmid-based expression and secretion vectors and study of the immobilization of proteins on the surface of Bacillus subtilis cells
… constructed allowing expression of recombinant proteins in the cytoplasm and their secretion into the culture supernatant. The author has also established an experimental system to immobilize proteins on the cell wall of B. subtilis. The sorting of surface proteins to the cell wall in B. …
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Elaboration of 4′-Phosphopantetheine Adenylyltransferase inhibitors and Benzoxaboroles as antimalarial agents
… beyond those involving Co-A biosynthesis. Penicillin binding protein (PBP) inhibitors from an antibacterial programme at H3D were screened for antiplasmodium activity. This identified a hit compound H3D006145 (43/MM2-92) with activity against PfNF54 (IC50 = 1.06 µM) and no cytotoxicity …
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Structural and Spectroscopic Characterization of Modular Photoreceptors
Photoreceptors are signaling proteins that perceive a light signal and generate light-dependent biochemical responses. Cyanobacteriochromes (CBCRs) from photosynthetic cyanobacteria are an emerging family of bilin-based photoreceptors that are compact in size and diverse in absorption spectra. …