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Showing 1 to 20 of 20 for “"pc-12"”.

  1. Temporal evolution of intracellular signaling and gene expression following patterns of membrane depolarization in the pheochromocytoma cell line, PC 12

    … on undifferentiated pheochromocytoma (PC 12) cells. In response to chronic depolarization, cytoplasmic free Ca2+ ([Ca2+]i) levels increased transiently, then decreased to intermediate levels that were maintained for more than 90 minutes. Short pulses of depolarization, from 1 to 5 …

    mit Repository record for Temporal evolution of intracellular signaling and gene expression following patterns of membrane depolarization in the pheochromocytoma cell line, PC 12 (opens in a new tab)

  2. The growth-arrest-specific protein gas7 potentiates neuronal differentiation

    … neuroprotective activity were investigated in PC 12 and NT2 cells. gas7 overexpression in PC12 cells promoted neurite outgrowth and potentiated nerve growth factor-induced expression of the neuronal markers betaIII-tubulin, synaptotagmin, alpha7 subunit of the acethylcholine receptor, and …

    ottawa-retro Repository record for The growth-arrest-specific protein gas7 potentiates neuronal differentiation (opens in a new tab)

  3. Evaluation of Prototype Cell Delivery Catheters Using Agarose Gel and Cell Culture Experiments

    … We initially delivered pheochromocytoma (PC 12) cells through a 25-gauge syringe needle to optimize cell growth. We observed in the agarose gel experiments that when the inner catheter was filled and then inserted with the outer catheter into gel, no air bubble or backtracking of dye was …

    vcu Repository record for Evaluation of Prototype Cell Delivery Catheters Using Agarose Gel and Cell Culture Experiments (opens in a new tab)

  4. Mechanism of Action of Antipsychotics, Haloperidol and Olanzapine in vitro

    … cytotoxicity in a neuronal cell model using PC-12 cells. HP was found to be ineffective as a superoxide radical scavenger but appeared to be a potent scavenger of hydroxyl radicals with a rate constant of ~6.78 X 109 M-1s-1. Olz on the other hand was found to scavenge hydroxyl radical at a …

    vt Repository record for Mechanism of Action of Antipsychotics, Haloperidol and Olanzapine in vitro (opens in a new tab)

  5. Architectural, mechanical and biochemical control of collagen films and scaffolds to guide neural- and fibroblast cell behaviour

    … using rat Schwann cells (RSC96), model neuronal (PC-12) cells and human dermal fibroblasts (HDF). Fluorescence imaging and lactate dehydrogenase assays revealed that elastin incorporation and lower levels of cross linking led to an increase in the attachment of RSC96 and PC-12 cells. Elastin …

    cambridge Repository record for Architectural, mechanical and biochemical control of collagen films and scaffolds to guide neural- and fibroblast cell behaviour (opens in a new tab)

  6. Examination of Runway Occupancy Times for General Aviation Aircraft

    … category. These aircraft are the Pilatus PC-12, the Cessna Corvalis, the Cirrus SR-22, and the Piaggio Avanti. Data collection occurred at Rocky Mountain Metropolitan Airport (KBJC) in Broomfield, Colorado, with a computer program that stored various times into a database. The stored times …

    nodak Repository record for Examination of Runway Occupancy Times for General Aviation Aircraft (opens in a new tab)

  7. Identification of novel target genes for the plasticity-related transcription factor Zif268

    … levels of zif268 by transient transfection in PC-12 cells and NMDA stimulation in primary cultured cortical neurones. In addition, ubiquilin mRNA and protein levels were also downregulated within the same experimental paradigms, implying that both gephyrin and ubiquilin are downstream …

    glasgow Repository record for Identification of novel target genes for the plasticity-related transcription factor Zif268 (opens in a new tab)

  8. Functional thin film polymers for biopassivation of neuroprosthetic implants

    … that the material is biocompatible with PC 12 neurons. The second developed material, poly(pentfluorophenyl methacrylate-co-ethylene glycol diacrylate), was developed as a platform for easy surface functionalization of neural probe coatings with bioactive molecules.

    mit Repository record for Functional thin film polymers for biopassivation of neuroprosthetic implants (opens in a new tab)

  9. Neuroprotective Effects of FGF20 on Dopamine Neurones

    … in the nigrostriatal tract. Additionally, in PC 12 cells, FGF20’s neuroprotective effects against 6OHDA toxicity were demonstrated to be mediated by the FGFRs at the receptor level, and by the ERK1/2 MAPK pathway at the intracellular level. Others have shown the heparin sulphate proteoglycan, …

    kings Repository record for Neuroprotective Effects of FGF20 on Dopamine Neurones (opens in a new tab)

  10. Neuroprotective Effects of Sulforaphane and the Involvement of Autophagy

    … the bioactivities of SFN on neuronal cells. PC-12 and SH-SY5Y cells were used to resemble neuronal-like systems to research especially autophagy as well as nuclear factor E2-related factor 2 (Nrf2) and how these pathways might influence neuroprotective abilities of SFN. Basal assessments …

    east-anglia Repository record for Neuroprotective Effects of Sulforaphane and the Involvement of Autophagy (opens in a new tab)

  11. Chronic low-level Pb exposure during development alters proteins involved in energy metabolism in auditory neurons of the brainstem

    … Both differentiated SH-SY5Y cells and PC-12 cells exposed to 10 μM Pb for 48 h result in a significant decrease in VDAC expression. Exposure to 24 h of hypoxia fails to decrease VDAC expression, suggesting this is a specific effect of Pb. In addition, a corresponding decrease in …

    montana-tech Repository record for Chronic low-level Pb exposure during development alters proteins involved in energy metabolism in auditory neurons of the brainstem (opens in a new tab)

  12. Chronic low-level Pb exposure during development alters proteins involved in energy metabolism in auditory neurons of the brainstem

    … Both differentiated SH-SY5Y cells and PC-12 cells exposed to 10 μM Pb for 48 h result in a significant decrease in VDAC expression. Exposure to 24 h of hypoxia fails to decrease VDAC expression, suggesting this is a specific effect of Pb. In addition, a corresponding decrease in …

    montana Repository record for Chronic low-level Pb exposure during development alters proteins involved in energy metabolism in auditory neurons of the brainstem (opens in a new tab)

  13. Evaluation of inhibitors of histone deacetylases as potential neurotrophic agents

    … on Neuroscreen-1 (NS-1) cells, a subclone of PC-12 (rat pheochromocytoma) cells. </p> <p>The HDAC inhibitors were tested alone and in combination with nerve growth factor (NGF). An in vitro method has been standardized that measures neurite outgrowth along with cytotoxicity of test compounds …

    mississippi Repository record for Evaluation of inhibitors of histone deacetylases as potential neurotrophic agents (opens in a new tab)

  14. Effects of Dextromethorphan on Neurite Outgrowth and Marble Burying Behavior

    … to the assessment of neurite outgrowth in PC12 cells. Therefore, to test the role of DM, a sigma-1 compound, glutamate NMDA antagonist, and DM metabolite-dextrorphan were used in both MBT and a neurite outgrowth assay. Marble burying data revealed a significant decrease in marbles buried …

    nmu Repository record for Effects of Dextromethorphan on Neurite Outgrowth and Marble Burying Behavior (opens in a new tab)

  15. Localisation and expression pattern of the Nogo receptor and its ligand, Nogo-A in cells of the mammalian central nervous system

    … receptor and flotillin in membranes of axons and PC-12 cells. Further more, extraction Nogo receptor antigen from rat brain using receptor bound protein-A sepharose beads, followed by probing with anti-flotillin antibody, established the link between lipid rafts and Nogo receptor.

    cape-town Repository record for Localisation and expression pattern of the Nogo receptor and its ligand, Nogo-A in cells of the mammalian central nervous system (opens in a new tab)

  16. Charakterisierung des 5-HT<sub>3B</sub>-Promotors der Ratte vor dem Hintergrund eines mit Chemotherapie-induziertem Erbrechen assoziierten Polymorphismus

    … wurde in der Ratten-Phäochromozytom-Zelllinie PC-12 und in der menschlichen Zelllinie HEK-293 charakterisiert. Dafür wurden RACE-basierte Transkriptionsstartseitenanalysen, in vitro- und in vivo-DNA-Methylierungsanalysen, bioinformatische Analysen, DNase I Hypersensitivity Assays, …

    goettingen Repository record for Charakterisierung des 5-HT<sub>3B</sub>-Promotors der Ratte vor dem Hintergrund eines mit Chemotherapie-induziertem Erbrechen assoziierten Polymorphismus (opens in a new tab)

  17. Development of new photoluminescent sensing materials, systems and applications for cell analysis

    … and confocal laser-scanning FLIM/PLIM-TCSPC microscope. The merits and limitations of each platform, including instrumental factors, analytical performance, lifetime calculation methods, potential sources of error were assessed. The third experimental section presents a new …

    cork Repository record for Development of new photoluminescent sensing materials, systems and applications for cell analysis (opens in a new tab)