Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"patient derived organoids"”.
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IDENTIFICATION OF CANCER- AND METASTASIS-RELATED EPIGENETIC DYNAMICS USING PATIENT-DERIVED ORGANOIDS
… selectively activated in colorectal cancer (CRC) patient-derived organoids (PDOs) with respect to the normal counterpart that are also conserved among different CRC subtypes, suggesting the presence of shared epigenetic mechanisms that are activated despite heterogeneous mutational landscapes. …
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PATIENT-DERIVED ORGANOIDS AND EXTRACELLULAR VESICLES: UNLOCKING NEW FRONTIERS IN BREAST CANCER DRUG TESTING AND BIOMARKER DISCOVERY
… BC are not satisfactorily tailored to individual patients and often result in failure. The main reason of that is tumor heterogeneity, resulting in different levels of aggressiveness and treatment sensitivity. To address this challenge, personalized medicine strategies are crucial; achieving this …
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Modelling pancreatic cancer in vivo with patient derived organoids & humanised mouse models for the investigation of immunotherapeutic treatment response
… the immunophenotypic variations between patients. In this dissertation I outlined the development of a novel preclinical model of human pancreatic cancer (hPC) that incorporated patient derived organoids (PDOs) with autologous splenic mononuclear cell (SPMC)-derived HIS mice. I first …
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Phenotypic dynamics of rectal cancer under neoadjuvant therapy: a multi-omic single-cell perspective from patient-derived organoids [Evoluzione fenotipica del carcinoma del retto durante terapia neoadiuvante: una prospettiva multi-omica a singola cellula da organoidi derivati dal paziente]
Questo studio esplora i meccanismi di adattamento fenotipico e l’eterogeneità intratumorale (ITH) nel carcinoma colorettale, con particolare attenzione alle forme localmente avanzate di carcinoma del retto. La resistenza terapeutica rappresenta una delle principali sfide in oncologia; comprenderne …
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Targeting the intestinal stem cell niche through energy and metabolism signaling, for colorectal cancer chemoprevention using aspirin and metformin
… further validated in mouse Apcfl/fl intestinal organoids, whereby aspirin and metformin reduced clonogenicity capacity. Patient organoids from different genetic backgrounds were established: sporadic, familial adenomatous polyposis (FAP), and Peutz- Jeghers syndrome (PJS). Upon treatment with …
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Characterising the evolutionary dynamics of hypermutated tumours using single-cell sequencing
… responses are only observed in a subset of patients. Therefore, advancing our understanding of the molecular mechanisms underpinning variable responses to ICB is critical. Previous clinical and preclinical studies of MMRD tumours showed that the clonality rather than the burden of mutations …
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Antioxidant Programs Are Essential for Enzalutamide-Resistant Prostate Cancer
… antagonists, such as enzalutamide, is common in patients with advanced prostate cancer (PCa). In order to identify which patients will develop therapy resistance and the underlying cause, it is critical to characterize detailed mechanisms of resistance to improve patient outcomes. To identify the …
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DISSECTING RESISTANCE TO IMMUNOTHERAPY IN MSS COLORECTAL CANCER
Patients with colorectal cancer (CRC) exhibiting DNA mismatch repair deficiency and microsatellite instability (MSI) often display favorable responses to immune checkpoint inhibitor (ICI) therapies, in contrast to mismatch repair proficient and microsatellite stable (MSS) tumors. While the high …
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Leveraging Single Cell Technologies For The Characterization and Treatment of Refractory Pancreatic Cancer
… isogenic pancreatic cancer (PDAC) cell lines, patient-derived organoids (PDOs), and patient tissue samples that contribute to the proliferation of resistant cell phenotypes and, ultimately, treatment failures in the clinic. I then apply single-cell characterization methods to a panel of PDO …
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PROBABILISTIC AND DEEP LEARNING APPROACHES TO MODELING BIOLOGICAL SYSTEMS
… computational analysis of lentivirally barcoded patient-derived organoids to show that epigenetic heritability plays a key role in drug resistance. This leads to a unified view where stable epigenetic differences drive diverse cell states and adaptive responses to treatment. The final part …
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Patient-derived and xenograft-derived organoids for characterization, drugs testing and biobanking of pediatric brain tumors
… tumor, have a higher incidence in younger patients, whereas older ones tend to present tumors of glial origin. Most of CNS-embryonal tumors are highly malignant and consequently leading to poor prognoses. Despite the understanding of the underlying molecular landscape of these tumors that …
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High throughput measurement and perturbation of tissues and tissue-derived cellular models
… cause disease. In vitro cellular models– such as patient derived organoids– that recapitulate tissue biology provide a platform for systematically testing these hypotheses. However, such experiments are difficult to scale, requiring the development of new technologies for perturbing complex model …
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A broadened HLA ligandome uncovers new immunotherapy targets for pancreatic cancer + A prime editor mouse to model a broad spectrum of somatic mutations
… this challenge through extensive profiling of patient-derived organoids with whole-genome sequencing, RNA sequencing, and immunopeptidomics. These data enable a proteogenomics approach that tailors MAP identification to each individual patient sample. Harnessing this platform, my colleagues and …
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The role of microRNA-194 in prostate cancer progression
… growth of aggressive models of NEPC, including patient-derived organoids. By integrating the miR-194 “targetome” with transcriptomic data, my work has provided important insights into miRNA function in cancer cells (Chapter 4). Specifically, I have found that miR-194 functions potently through …
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Alteration of differentiation in glioblastoma: from spatial transcriptomics approaches to the identification of a suppressor event
… which is evident both at intratumoral and inter-patient levels, represents a substantial obstacle to the achievement of effective treatments. In this context, the use of single-cell resolution appears one of the most powerful means for understanding the intricacies and unravelling the …
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Impaired ketogenesis is implicated in intestinal stem cell function and epithelial regeneration in ulcerative colitis
… inhibition (SCENITH) on treatment-naïve UC patient epithelial samples and on UC patient derived organoids. Single-cell quantitative immunofluorescence showed a loss of mitochondrial oxidative phosphorylation proteins Complex I and IV, necessary for ATP production in the UC epithelium. …
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ROLE OF HIGH DENSITY LIPOPROTEINS AGAINST PROSTATE CANCER
… therapy (ADT) is initially effective, many patients progress to castration-resistant disease with poor prognosis and limited treatment options, highlighting the need for new therapeutic strategies. The tumor microenvironment (TME) contains pro-oxidant, pro-inflammatory mediators and lipids …